A 2 cm tri-phase SPME fiber (50/30 μm DVB/Carboxen/PDMS, Supelco, Bellefonte, PA, USA) was used to collect and concentrate volatiles prior to running on an Agilent 6890 GC coupled with a 5973 N MS detector (Agilent Technologies, Palo Alto, CA, USA). Before analysis, samples were held at 4°C in a Peltier cooling tray attached to a MPS2 autosampler (Gerstel). All other volatile sampling and analysis methods were as previously described [15 ]. The volatile 3-hexanone was used as an internal control. An authentic γ-D standard (Sigma Aldrich, St. Louis, MO, USA) was run under the same chromatographic conditions as berry samples for verification of volatile identify. The area of each γ-D peak was normalized to the peak area of the internal standard, and normalized peak areas were compared between samples.
5973 n ms detector
Manufactured by Agilent Technologies
Sourced in United States
The 5973 N MS detector is a key component of Agilent's gas chromatography-mass spectrometry (GC-MS) systems. It is designed to provide sensitive and selective detection of a wide range of compounds. The 5973 N MS detector utilizes electron ionization (EI) technology to generate ions from the sample, which are then analyzed by a quadrupole mass analyzer to determine the mass-to-charge ratios of the analytes. This information can be used to identify and quantify the components in a complex sample.
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Lab products found in correlation
Agilent 6890 gc, by Agilent Technologies (4 mentions)
Mps2 autosampler, by Gerstel (3 mentions)
μm dvb carboxen pdms, by Merck Group (2 mentions)
System 2000 ft ir spectrophotometer, by PerkinElmer (1 mentions)
6890n chromatograph, by Agilent Technologies (1 mentions)
Hp 5ms capillary column, by Agilent Technologies (1 mentions)
Dvb carboxen pdms, by Merck Group (1 mentions)
5 protocols using 5973 n ms detector
1
GC-MS Analysis of Berry Volatiles
2
Volatile Compound Analysis of Fruit Samples
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A 2 cm tri-phase SPME fiber (50/30 μm DVB/Carboxen/PDMS, Supelco, Bellefonte, PA, USA) was used to collect and concentrate volatiles prior to analysis on an Agilent 6890 GC coupled with a 5973 N MS detector (Agilent Technologies, Palo Alto, CA, USA). Before analysis, samples were held at 10 °C in a Peltier cooling tray attached to a MPS2 autosampler (Gerstel). All other volatile sampling and analysis methods were as previously described [23 (link)]. An authentic MA standard (Sigma Aldrich, St. Louis, MO, USA) was run under the same chromatographic conditions as fruit samples for verification of volatile identity. MA was often found in low abundance and was therefore quantified using a Single Ion Mode method scanning for the major MA ion (ion 119). The peak area of ion 119 was quantified and normalized to the peak area of the internal standard. The normalized peak areas were compared between samples.
3
Synthesis of Zinc-based Complexes
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Ethylzinc benzoate,5e [(EtZn)2Zn(OAr)4],7 (link)N-(4-methoxy-benzylidene)aniline (1a ), N-benzylidene-4-methoxyaniline (1b )17 and tBu2Zn,18 (link) were prepared according to the literature procedures under a nitrogen atmosphere using standard Schlenk techniques. All other reagents were of commercial grade obtained from Sigma-Aldrich Co. The solvents and propionic acid used for preparation of the initiators were carefully dried and distilled under a nitrogen atmosphere prior to use. TEMPO was sublimated and stored under a nitrogen atmosphere. Oxygenation reactions were carried out using atmospheric air dried by passing it through a tube filled with anhydrous CaCl2/KOH. NMR spectra were acquired on a Varian Mercury 400 Spectrometer, and IR spectra were recorded on a Perkin-Elmer System 2000 FT-IR spectrophotometer. GC-MS analysis was performed on an Agilent 6890N chromatograph coupled with an Agilent 5973N MS detector.
4
Volatile Metabolite Profiling of EA105
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Volatile metabolites produced by EA105 were extracted using an SPME fused silica fiber coated with 65 μm of polydimethylsiloxane/divinylbenzene (Sigma-Adrich). EA105 was grown on LB agar for 2 days and then the fiber was exposed for 24 hours to the headspace above EA105. The fiber was then manually injected into an Agilent 6890 GC with a 5973 N MS detector (Agilent Technologies), installed with a HP-5MS capillary column (30 m × 0.25 mm, 0.5 μm) and a flame ionization detector. Inlet temperature was 250°C. Oven conditions started at 40°C for 2 min, ramped at 10°C/min to 250°C, and held for 2 min. VOCs were identified using the mass spectral library (NIST). Standard curves of the identified compounds were created using commercially available compounds. They were diluted in methanol and 2 μl was injected into the GC. The concentration of the volatiles produced was determined by comparing peak heights of the EA105 profile to the standard curve. Four biological replicates were performed.
5
Volatile Compound Extraction and Identification
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Volatiles were concentrated using a tri-phase SPME fiber (2 cm, 50/30 μm DVB/Carboxen/PDMS, Supelco, Bellefonte, PA, USA) running on an Agilent 6890 GC coupled with a 5973 N MS detector (Agilent Technologies, Palo Alto, CA, USA). Samples were maintained at 4 °C on a Peltier cooling tray attached to a MPS2 autosampler (Gerstel) prior to analysis. Other volatile sampling and analyses were performed as described previously [30 ]. The volatile 3-hexanone served as an internal control. An authentic methyl anthranilate standard (Sigma Aldrich, St. Louis, MO, USA) was run under the same chromatographic conditions to identify the correct peak.
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