the quantification of apoptosis of lymphocytes was performed by the method of flow cytometry on FACSCalibur device (“Весton Dickinson”) with the use of some parameters, such as the determination of the fragmentation of DNA by the iodide propidium (IP) (“Sigma”) [28 (link)], the translocation of membrane phosphatidylserines to the surface of lymphocytes using merocyanine (МС540), the measuring of the variation in mitochondrial membrane potential of lymphocytes according to the fluorescence intensity CMX-Ros (“Molecular Probes”) [29 (link)]. More than 10, 000 cells were counted on each variant of the experiment.
Merocyanine
Manufactured by Thermo Fisher Scientific
Sourced in United States
Merocyanine is a fluorescent dye used in various laboratory applications. It is a cyanine-based dye known for its ability to emit light when exposed to specific wavelengths of light. The core function of Merocyanine is to act as a fluorescent marker or label in various scientific experiments and analyses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using merocyanine
1
Apoptosis Quantification of Lymphocytes
2
Phosphotyrosine Antibody Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All media components and the ethidium homodimer stain were purchased from Sigma-Aldrich (Kempton Park, South Africa). The merocyanine and Yo-Pro 1 stains were purchased from Molecular Probes Inc. (Eugene, Oregon, USA; via Invitrogen).
The primary anti-phosphotyrosine antibody (Clone 4G10) and the lyophilized secondary antibody-Cy3 conjugate (isotype IgG2βκ) for detecting antiphosphotyrosine binding were purchased from Millipore (290 Concord Road, Billerica, MA 01821 USA).
The primary anti-phosphotyrosine antibody (Clone 4G10) and the lyophilized secondary antibody-Cy3 conjugate (isotype IgG2βκ) for detecting antiphosphotyrosine binding were purchased from Millipore (290 Concord Road, Billerica, MA 01821 USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!