Secondary anti human antibodies

Fluorescein isothiocyanate, phycoerythrin; allophycocyanin; allophycocyanin-Cy7 and PE/Dazzle labelled CD40, CCR1, IFN-γ, T-bet, IL-17A, IL-22, and TNF-α anti-human monoclonal antibodies; and red blood cell lysing, fixation, and permeabilizing buffers were purchased from BioLegend (San Diego, CA, USA). FcR blocking reagent was purchased from Miltenyi Biotech (Bergisch Gladbach, Germany). RORγt, GolgiStop, and acid−citrate−dextrose vacutainer tubes were purchased from BD Biosciences (San Diego, CA, USA). The primers used were purchased from GenScript (Piscataway, NJ, USA). TRIzol reagent was obtained from Life Technologies (Grand Island, NY, USA). The SYBR Green PCR Master Mix and High-Capacity cDNA Reverse Transcription kit was purchased from Applied Biosystems (Paisley, UK). The primary antibodies against CCR1 and secondary anti-human antibodies used for Western blotting were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Phorbol 12-myristate 13-acetate (PMA), ionomycin, phosphate-buffered saline (PBS), RPMI-1640 medium, Ficoll-Paque, and Hanks’ Balanced Salt Solution (HBSS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nitrocellulose membranes were obtained from Bio-Rad Laboratories (Hercules, CA, USA), and a chemiluminescence Western blot detection kit was purchased from GE Healthcare Life Sciences (Piscataway, NJ, USA).

DHT was obtained from Steraloids (Wilton, VA). Nrf1 antibody was obtained from Proteintech Group (Chicago, IL). Antibodies to Nrf2, AR, and TATA Binding Protein (TBP) were obtained from Abcam (Cambridge, MA). Isotype IgG (non-specific control) and all secondary anti-human antibodies were obtained from Santa Cruz (Santa Cruz, CA). Anti-V5 antibody was obtained from Invitrogen (Carlsbad, CA). The AR antibody used for ChIP assays was obtained from Active Motif (Carlsbad, CA). The Nrf1 specific siRNA and the non-specific control (NC1) siRNA were obtained from Integrated DNA Technologies (Coralville, IA; Cat# HSC.RNAI. N003204.12.1-3). Plasmids were obtained from the following sources: p65-Nrf1 expression vector (p65-Nrf1-V5His) was a gracious gift from Dr. Chan [30] , the p120-Nrf1-V5His expression vector was obtained from Dr. Zhang [38] and the psPSA-Luc reporter (firefly luciferase) vector was obtained from Dr. Abdel-Mageed’s laboratory [39] . The pRL-TK (renilla luciferase) vector was purchased from Promega (Madison, WI). The Nrf2 expression vector (pCMV6-Nrf2) was purchased from Origene (Rockville, MD) and pcDNA3.1 control vector was purchased from Invitrogen (Carlsbad, CA).