Log In Sign up
The largest database of trusted experimental protocols

15n alanine

Manufactured by Cambridge Isotopes
Visit Supplier

15N-alanine is a stable isotope-labeled amino acid produced by Cambridge Isotopes. It contains a 15N isotope, which can be used as a tracer in various analytical and research applications.

Automatically generated - may contain errors

Lab products found in correlation

U 13c glucose, by Cambridge Isotopes (1 mentions) U 13c glutamine, by Cambridge Isotopes (1 mentions) 15n glutamine, by Cambridge Isotopes (1 mentions) Endotoxin free maxiprep kit, by Qiagen (1 mentions)

2 protocols using 15n alanine

1

Mouse Transcription Factor Overexpression and Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
The constructs for mouse injection, including pT3-EF1α-c-MYC, pT3-EF1α-MCL1 and pCMV-SB (which encodes for sleeping beauty transposase), and pCMV-CRE (which encodes for CRE recombinase), were previously described58 . miR30-based shRNA targeting for Gls2 and Renilla Luciferase were cloned into pT3-EF1α-c-MYC. The shRNA sequences are: Gls2: CATCATGCCAACAAGCAACTT and Renilla Luciferase: AGGAATTATAATGCTTATCTA. All plasmids used for in vivo experiments were purified using the Endotoxin-free Maxiprep kit (Qiagen). [U-13C]-glucose, [U-13C]-glutamine, 15N-glutamine and 15N-alanine were purchased from Cambridge Isotope Laboratories, Inc.
Méndez-Lucas A., Lin W., Driscoll P.C., Legrave N., Novellasdemunt L., Xie C., Charles M., Wilson Z., Jones N.P., Rayport S., Rodríguez-Justo M., Li V., MacRae J.I., Hay N., Chen X, & Yuneva M. (2020). Identifying strategies to target the metabolic flexibility of tumours.. Nature metabolism, 2(4), 335-350.
+ Open protocol
+ Expand
2

Whole Body Protein Turnover Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole body protein turnover was measured by a single‐pool whole body method as previously described (Berryman et al., 2017 (link); Ferrando et al., 1996 (link)). Briefly, after providing a urine sample to correct for background isotope enrichments, volunteers ingested a single dose of [15N]alanine (99% enriched; Cambridge Isotope Laboratories, Andover, MA) at 4 mg 15N/kg body mass after consuming their evening meal. Volunteers were instructed to fast and collect their urine for the next 10–12 h, ending with the first void the following morning. Nitrogen flux (Q; g N/24 h) was determined using urinary urea enrichment according to Fern et al. (1985 (link)). Protein synthesis (PS) and breakdown (PB) were calculated according to Stein et al. (1991 (link)).
Berryman C.E., McClung H.L., Sepowitz J.J., Gaffney‐Stomberg E., Ferrando A.A., McClung J.P, & Pasiakos S.M. (2022). Testosterone status following short‐term, severe energy deficit is associated with fat‐free mass loss in U.S. Marines. Physiological Reports, 10(18), e15461.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!