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2 protocols using dihydro β erythroidine

1

Radioligand Binding Assay Reagents

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[7,8-3H]Dopamine (21.2 Ci/mmol (0.784 TBq/mmol)) and [1-7,8-3H]noradrenaline (12.1 Ci/mmol (0.448 TBq/mmol)) were purchased from Perkin Elmer, Monza, Italy. Nicotine hydrogen tartrate salt, choline, 4-aminopyridine, 6-nitroquipazine, desipramine, beta-amyloid (40-1), horseradish peroxidase-conjugated anti-mouse secondary antibody, Ponceau Red were from Sigma (Sigma-Aldrich, St Louis, MO, USA); Monoclonal antibody 6E10 was from Covance ImmunoTechnologies, Dedham, MA; 5-iodo-A-85380, dihydro-β-erythroidine, α-conotoxin MII, oxotremorine sesquifumarate, desformylflustrabromine hydrochloride, galantamine hydrobromide, SR 165845, beta-amyloid (1-40) were from Tocris (Tocris Bioscience, Bristol, UK).
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2

Solubilization of Pharmacological Agents

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Bumetanide and forskolin were dissolved in ethanol (final maximal volume concentration of ethanol 2.5 mL/L). Calmidazolium, darifenacin, J104129 (Tocris, Bristol, UK), and trifluoperazine were dissolved in dimethylsulfoxide (DMSO; final maximal DMSO volume concentration 2.5 mL/L). Tetrodotoxin was dissolved in 2 × 102 mol/L citrate buffer. Acetylcholine chloride, acetylthiocholine chloride, atropine, α-bungarotoxin (Tocris), carbachol, choline, dihydro-β-erythroidine (Tocris), DMPP, hexamethonium, nicotine hydrogen tartrate, pirenzepine, strychnine, and telenzepine were dissolved in distilled water. If not indicated differently, drugs were purchased from Sigma, Taufkirchen, Germany.
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