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P19arf sc 32748

Manufactured by Santa Cruz Biotechnology
Sourced in United States

P19Arf (sc-32748) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect the expression of the p19Arf protein, which is a tumor suppressor involved in cell cycle regulation and apoptosis.

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2 protocols using p19arf sc 32748

1

Murine Skin Papilloma Immunohistochemistry

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Murine skin papillomas obtained with a DMBA/TPA carcinogenesis protocol and normal skin controls were processed for immunohistochemistry as described 63 (link), using the following antibodies: p19Arf (sc-32748, Santa Cruz), SIX1 (HPA001893, Sigma), Ki-67 (0003110QD, Master Diagnostica) and Keratin 10 (PRB-159P, Covance).
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2

Western Blot Analysis of Cell Signaling Proteins

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Western blot analysis was performed as described11 (link) with nitrocellulose membranes probed for p16 (m156, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phosphorylated p53 (9284, 1:2000, Cell Signaling, Danvers, MA, USA), total p53 (CM5, 1:2000, Leika, Biosystems, Richmond, IL, USA), phospho-histone γ-H2AX (1:1000, Upstate, Cell Signaling Solutions, Temecula, CA, USA), p19ARF (sc-32748, 1:500, Santa Cruz Biotechnology), TUBA1B (DM 1A, 1:2500, Sigma-Aldrich Chemical Company, St Louis, MO, USA), GAPDH (8884, 1:10000, Cell Signaling) and/or ACTB (A5060, 1:1000, Sigma-Aldrich Chemical Company).
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