Ldh assay kit wst ck12

The integrity of the cell membrane related to LDH release was assessed by the LDH Assay Kit-WST (CK12, Dojindo Molecular Technologies, Inc., Rockville, MD, USA), according to the manufacturer’s instructions. The working solution (100 µL) was added to the cell suspension (50 µL), followed by a 30 min incubation at room temperature in the dark. The absorbance was measured with a microplate reader (model 680, Bio-Rad, Hercules, CA, USA) at 490 nm, and the LDH content, expressed as a percentage of cytotoxicity, was calculated using the following equation: (Test Substance − Low Control)/(High Control − Low Control) × 100. Experiments were performed with n = 4 replicates.

The lactate dehydrogenase (LDH) releasing was evaluated as cell damage by LDH Assay Kit-WST CK12 (CK12, DOJINDO, Kumamoto, Japan). Injury rate (%) of cancer cells was calculated as: (experimental LDH release-spontaneous LDH release)/(total LDH release-spontaneous LDH release).