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Anti cd19 6d5

Manufactured by Southern Biotech
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Anti-CD19 (6D5) is a monoclonal antibody that binds to the CD19 antigen. CD19 is a cell surface protein expressed on B cells and is involved in B cell regulation and signaling. The Anti-CD19 (6D5) antibody can be used for the detection and analysis of CD19-positive cells in research applications.

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Lab products found in correlation

Fixable viability dye, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg h l, by Southern Biotech (2 mentions)

2 protocols using anti cd19 6d5

1

Serum IgG Binding Assay

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Serum was collected from mice and stored at -20o C. 5x105 2W:OVA or Kd-expressing splenocytes in PBS 2%FBS were incubated with 5μL serum for 20 min at RT. Cells were washed, then stained with a fixable viability dye (Invitrogen), anti-CD19 (6D5), and goat anti-mouse IgG (H+L) (catalog 1031–02, Southern Biotech) for 15 min at RT. Relative IgG was determined by the MFI of live CD19- cells.
Wang P., Chen L., McIntosh C.M., Lane J.I., Li R., Xie S.Z., Sattar H., Esterhazy D., Chong A.S, & Alegre M. (2022). Oral alloantigen exposure promotes donor‐specific tolerance in a mouse model of minor‐mismatched skin transplantation. American Journal of Transplantation, 22(10), 2348-2359.
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2

Quantifying Serum IgG Levels in Mice

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Serum was collected from mice and stored at −20°C. 5 × 105 2W:OVA or Kd‐expressing splenocytes in PBS 2%FBS were incubated with 5 μl serum for 20 min at RT. Cells were washed, then stained with a fixable viability dye (Invitrogen), anti‐CD19 (6D5), and goat anti‐mouse IgG (H + L) (catalog 1031–02, Southern Biotech) for 15 min at RT. Relative IgG was determined by the MFI of live CD19− cells.
Wang P., Chen L., McIntosh C.M., Lane J.I., Li R., Xie S.Z., Sattar H., Esterhazy D., Chong A.S, & Alegre M. (2022). Oral alloantigen exposure promotes donor‐specific tolerance in a mouse model of minor‐mismatched skin transplantation. American Journal of Transplantation, 22(10), 2348-2359.
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