Rabbit anti

Total protein was extracted from peritoneal macrophages using RIPA lysis buffer (Beyotime, Shanghai, China) with phenylmethanesulfonyl (PMSF; Beyotime) and cocktail (Sigma-Aldrich). The total protein in each sample was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose filter membranes (HATF00010; MilliporeSigma, Burlington, MA, USA). The membranes were incubated with primary antibodies at 4 °C after blocking with 5% skim milk. The primary antibodies were rabbit anti-AMP-activated protein kinase (AMPK) alpha1 (phospho T183) + AMPK alpha2 (phospho T172) (Abcam, Cambridge, UK), rabbit anti-AMPK (Abcam) and mouse anti-β-actin (Abcam). On the following day, the membranes were incubated with the horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) or goat anti-mouse IgG (Zsbio, Beijing, China) at 25 °C for 1 h. The images were acquired using a protein imaging system (Proteinsimple, lourCherm FC3, China).

The XYW (Tai Ji, China, batch number 1707029) and fluoxetine hydrochloride (FLX) (Patheon, France, 7686 A) were dissolved in pure water solution preparation (SOD, batch number 20180,309), malondialdehyde (MDA, batch number 20180,313) (GSH, batch number 20180,621), and GSH/glutathione disulfide (GSH/GSSG, batch number 20180,726) levels were assessed using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Nitric oxide (NO) assay kit (Biyuntian, 0,72117,171,110, China). Rat interleukin 6 (IL6) ELISA Kit (MULTI SCIENCES, A30680231), rat interleukin 1β (IL1B) ELISA Kit (ExCell Bio, 21H183), rabbit anti-NTRK2 antibody (Cell Signaling Technology; Cat. No. #4603), rabbit anti-AKT1 antibody (Cell Signaling Technology; Cat. No. #4691S), rabbit anti-p-AKT1 antibody (Cell Signaling Technology; Cat. No. #4060S), rabbit anti-HMOX1 antibody (Cell Signaling Technology; Cat. No. #S2206S), rabbit anti-PIK3CA antibody (Servicebio; Cat. No. #LS190932), rabbit anti-GAPDH antibody (Servicebio; Cat. No. #GB11002), rabbit anti-SOD1 antibody (Novus Biologicals; Cat. No. #03253462C2B), rabbit anti-NFE2L2 antibody (Invitrogen; Cat. No. #TK2668681A), rabbit anti-brain-derived neurotrophic factor (BDNF) antibody (Abcam; Cat. No. #ab108319) and anti-rabbit IgG HRP-linked antibody (Servicebio; Cat. No. #GB23303).

Mouse anti-Myc monoclonal antibody, rabbit anti-Flag monoclonal antibody and mouse anti-β-actin monoclonal antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-Flag monoclonal antibody was purchased from Sigma–Aldrich (St. Louis, MO, USA). rabbit anti-LGP2 polyclonal antibody and rabbit anti-eukaryotic translation initiation factor 4 gamma (eIF4G) polyclonal antibody were purchased from Abcam(Cambridge, MA, USA). Anti-VP1 polyclonal antibody was prepared by our laboratory (unpublished data). The full-length cDNA of LGP2 was amplified from PK-15 cells and cloned into pcDNA^TM3.1/myc-His(-)A vector (Invitrogen, Carlsbad, CA, USA) to generate the Myc-tagged expressing plasmid (Myc-LGP2). Various Flag-tagged viral protein expressing plasmids were constructed by our lab previously as described.^{3 (link)} A series of Myc-tagged LGP2 mutant constructs were generated by site-directed mutagenesis PCR.⁵⁹ All the generated expressing plasmids were analysed and verified by DNA sequencing.