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Cxcl12 sdf 1 quantikine elisa kit

Manufactured by R&D Systems
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The CXCL12/SDF-1 Quantikine ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the quantitative determination of human CXCL12/SDF-1 concentrations in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Csb el003694hu, by Cusabio (1 mentions) Mouse milliplex kit, by Merck Group (1 mentions) Vegf quantikine elisa kit, by R&D Systems (1 mentions) Elisa reader, by Bio-Rad (1 mentions) Il 6 quantikine elisa kit, by R&D Systems (1 mentions)

Close spelling variants of this product

Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit Human CXCL12/SDF-1 alpha Quantikine ELISA Kit CXCL12/SDF-1 CXCL12 ELISA kits Quantikine ELISA kit SDF-1 Quantikine ELISA Quantikine kits CXCL12 Quantikines

2 protocols using cxcl12 sdf 1 quantikine elisa kit

1

Quantification of Biomarkers in Mouse Blood

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Peripheral blood levels of C‐peptide and insulin were assessed using a Mouse Milliplex kit (MMHMAG‐44 K, Millipore, Burlington, MA, USA), atrial natriuretic peptide (ANP) was determined using EIA (RAB00385, Sigma‐Aldrich), and SDF‐1 was measured using an ELISA kit (CXCL12/SDF‐1 Quantikine ELISA Kit, DY350‐05, R&D Systems). BPIFB4 protein levels were determined using an ELISA kit (CSB‐EL003694HU, Cusabio Technology, Houston, TX, USA). According to the vendor, the ELISA has an excellent specificity for the human protein, but cross‐reaction with the mouse protein cannot be excluded.
Dang Z., Avolio E., Thomas A.C., Faulkner A., Beltrami A.P., Cervellin C., Carrizzo A., Maciag A., Gu Y., Ciaglia E., Finato N., Damato A., Spinetti G., Alenzi A., Paisey S.J., Vecchione C., Puca A.A, & Madeddu P. (2020). Transfer of a human gene variant associated with exceptional longevity improves cardiac function in obese type 2 diabetic mice through induction of the SDF‐1/CXCR4 signalling pathway. European Journal of Heart Failure, 22(9), 1568-1581.
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2

ELISA Assays for Angiogenic Factors

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ELISA assays were carried out utilizing SFCM according to the manufacturer’s recommendations. Briefly, the plates were coated with the SFCM for 2 h and then were washed and incubated with the protein conjugate for 1 h. After washing, the substrate solution was added for 30 min, and then the stop solution was added and the plate was immediately read using a microplate (ELISA) reader (Bio-Rad) at 450 nm. VEGF Quantikine ELISA Kit, CXCL12/SDF-1 Quantikine ELISA Kit, CXCL8/IL-8 Quantikine ELISA Kit and IL-6 Quantikine ELISA Kit were obtained from R&D Systems, Minneapolis, MN, USA.
Aljagthmi W.A., Alasmari M.A., Daghestani M.H., Al-Kharashi L.A., Al-Mohanna F.H, & Aboussekhra A. (2024). Decorin (DCN) Downregulation Activates Breast Stromal Fibroblasts and Promotes Their Pro-Carcinogenic Effects through the IL-6/STAT3/AUF1 Signaling. Cells, 13(8), 680.
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