L lactic acid

Manufactured by Thermo Fisher Scientific

Sourced in France, United States, China, Belgium

L-lactic acid is a chemical compound that serves as a core component in various laboratory processes. It functions as a pH regulator and metabolic intermediate, playing a crucial role in various analytical and experimental applications.

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Lab products found in correlation

11 protocols using l lactic acid

Fermentation Dynamics Photometric Analysis

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A Thermo Scientific Gallery
discrete photometric analyzer was used to evaluate fermentation dynamics.
Reagent systems including d-glucose (ref 984304), d-fructose (ref 984302), ethanol (ref 984300), l-lactic acid
(ref 984308), and acetic acid (ref 984318) were purchased from Thermo
Fisher Scientific. Undiluted fermentation broth samples, excluding
the biomass (2 mL), were evaluated every 3 days for 30 days for each
isolate. All tests were performed at 37 °C using a 340 nm filter.
Findings were reported by means of triplicate measurements and standard
deviations using Microsoft Excel for Office 365 MSO version 1811.

Wickramasinghe P.C, & Munafo JP J.r. (2020). Fermentation Dynamics and Benzylic Derivative Production in Ischnoderma resinosum Isolates. ACS Omega, 5(35), 22268-22277.

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Comprehensive Metabolic Profiling Protocol

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The serum glucose (Glucose GOD-POD, ref 981,780, Thermo Fisher Scientific, Dardilly, France), uric acid (Uric Acid AOX, ref 981,788, Thermo Fisher Scientific, Dardilly, France), triglyceride (ref 981,786, Thermo Fisher Scientific, Dardilly, France), non-esterified fatty acid (NEFA-HR(2), FUJIFILM, Sobioda, Montbonnot Saint Martin) and hydroxybutyrate (RANBUT, RB 1007/MD, RANDOX, Roissy) concentrations; the serum total antioxidant status (TAS, NX2332, RANDOX, Roissy); the creatine kinase activity (CK-NAC, ref 92,307, BIOLABO, Abliance, Compiègne, France); and the liver glycolytic potential (Glucose HK, ref 981,779 and L-lactic acid, ref 984,308, Thermo Fisher Scientific, Dardilly, France) were measured spectrophotometrically using commercial kits.

Petit A., Tesseraud S., Collin A., Couroussé N., Berri C., Bihan-Duval E.L, & Métayer-Coustard S. (2024). Ontogeny of hepatic metabolism in two broiler lines divergently selected for the ultimate pH of the Pectoralis major muscle. BMC Genomics, 25, 438.

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Synthesis and Characterization of NADESs

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All chemical substances for NADESs preparation (betaine, proline, cholinium bitartrate, choline acetate (hydrogen bond acceptors — HBAs), ethylene glycol, citric acid, glycerol, levulinic acid, L-lactic acid, lactic acid, malic acid, imidazole, glycolic acid, diglycolic acid (hydrogen bond donors — HBDs) were purchased from Alfa Aesar GmbH. Purity of these chemicals was > 99%. NADESs synthesis followed the methodologies described by Hayyan et al. (2013b (link)) and Colombo Dugoni et al (2020 (link)). Table 1 illustrates composition and molar ratios of the NADESs assessed in this study.Table 1

Structure of NADESs prepared in this study

Vieira Sanches M., Freitas R., Oliva M., Mero A., De Marchi L., Cuccaro A., Fumagalli G., Mezzetta A., Colombo Dugoni G., Ferro M., Mele A., Guazzelli L, & Pretti C. (2022). Are natural deep eutectic solvents always a sustainable option? A bioassay-based study. Environmental Science and Pollution Research International, 30(7), 17268-17279.

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Anhydrous Lanthanide Chloride-Based Deep Eutectic Solvents

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LnCl₃ (Ln = Nd,
Eu, Dy, Er, Yb, anhydrous, ≥99.9%), LnCl₃.6H₂O (Ln = Nd, Eu, Dy, Er, Yb; ≥99.9%), choline chloride
(98%), ethylene glycol (anhydrous, 99.8%), and urea (99.5%) were purchased
from Sigma-Aldrich. l-lactic acid (anhydrous, 98%) was purchased
from Alfa Aesar. All anhydrous chemicals were transferred to an Ar
atmosphere glovebox [(O₂), (H₂O) ≤ 5
ppm] for use and storage. choline chloride and urea were dried in
a vacuum oven (24 h, 80 °C) before being transferred into the
glovebox.

Amphlett J.T., Lee Y., Yang W., Kang D., Sung N.E., Park J., Jung E.C, & Choi S. (2021). Spectroscopic Study into Lanthanide Speciation in Deep Eutectic Solvents. ACS Omega, 7(1), 921-932.

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Apple Fiber Pretreatment and Enzymatic Hydrolysis

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Choline Chloride, ChCl (98+%), L-lactic acid (85%–90% in water), were purchased by Alfa Aesar (Massachusetts, United States). l-Arginine (≥98%), acetic acid (≥99.8%), dry DMSO (99.9%) and ethanol (99.8%) were purchased from Sigma-Aldrich (Germany). Choline hydroxide in water solution (47%–50%) was purchased by TCI (Belgium), while 1-butyl-3-methylimidazolium (BMIM) methylcarbonate (98%) methanol solution was purchased from Proionic GmbH (Austria).
Cellulase from Trichoderma reesei (≥1 unit/mg) was purchased from Sigma Aldrich (Germany). Tris buffer (Ultra-pure), sodium hydroxide (98%), glucose (99%), sodium-potassium tartrate (99%), carboxyl methyl cellulose and dinitrosalicylic acid (98%) was purchased from Fisher Scientific (New Hampshire, United States).
Dried Apple fibers (pulp) were supplied by ILVO (Belgium). They were obtained by freeze-drying apple pomace collected after fruit juice production by Konings (Belgium). Then, the dried pomace were de-stoned, de-steal and pre-grinded and sieved (<1 mm). Before use, they were further grounded with blasting milling. These pretreatments allowed to obtain a more homogenous and manageable materials to facilitate the further processing.

Mero A., Moody N.R., Husanu E., Mezzetta A., D’Andrea F., Pomelli C.S., Bernaert N., Paradisi F, & Guazzelli L. (2023). Challenging DESs and ILs in the valorization of food waste: a case study. Frontiers in Chemistry, 11, 1270221.

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Zn(II)-Hmim Nanoparticle Synthesis

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2-Methylimidazole (Hmim) was purchased from Acros Organics. Zinc acetate dihydrate (Zn(OAc)₂·2H₂O) was obtained from Sigma-Aldrich. l-(+)-Lactic acid (85–90% aqueous solution) was purchased from Alfa Aesar. Sterile water (H₂O, Solopharm, Russia) and 0.9% saline solution (NaCl, MosFarm, Russia) were purchased from a pharmacy store. PBS buffer was prepared by dissolving a 5 g Gibco® PBS Tablet (Thermo Fisher Scientific, USA) in 500 mL of distilled water. Cell cultures A549 and HDF were obtained from ATCC. Cell culture media DMEM (Biolot, Russia) and phosphate-free DMEM (Thermo Fisher Scientific, USA) were used for in vitro assays. Gentamicin additive (50 μg mL⁻¹) was used as an antibiotic.

Butonova S.A., Ikonnikova E.V., Sharsheeva A., Chernyshov I.Y., Kuchur O.A., Mukhin I.S., Hey-Hawkins E., Vinogradov A.V, & Morozov M.I. (2021). Degradation kinetic study of ZIF-8 microcrystals with and without the presence of lactic acid. RSC Advances, 11(62), 39169-39176.

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Synthesis of Metal Chalcogenide Catalysts

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Zinc acetate dehydrate (Zn(CH₃COO)₂·2H₂O, ≥98%), sodium molybdate dehydrate (Na₂MoO₄·2H₂O, ≥99%) and hydrazine hydrate (N₂H₄·H₂O, 99.99%) solution were obtained from Sigma-Aldrich. Indium chloride (InCl₃, 99.995%), L(+) lactic acid (90%) and selenium (99.5+%) were obtained from ACROS Organics. Thioacetamide (C₂H₅NS, >98%) was obtained from TCI. All of the reagents were used as received without further purification. The DI water used in the catalyst preparation was from local sources.

Yang M.Q., Xu Y.J., Lu W., Zeng K., Zhu H., Xu Q.H, & Ho G.W. (2017). Self-surface charge exfoliation and electrostatically coordinated 2D hetero-layered hybrids. Nature Communications, 8, 14224.

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Evaluation of Antioxidant Properties

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Ch (>90% deacetylation) was obtained from Golden-Shell Co. China and L(+)-Lactic acid (90%) was obtained from Acros Organics (Belgium). Organic virgin FO—Nature foods (nutritional value per 100 g: 11 g saturated, 18 g monounsaturated and 71 g polyunsaturated fatty acids); OO (Origanum compactum)—Biover; and TO—Integralia were all purchased from Celeiro (Dietimport S.A., Portugal). Di-terc-butyl-methyl phenol (BHT), trichloroacetic acid (TCA) and 2-thiobarbituric acid reagent (TBA) were purchased from Merck (Germany).

Mendes C.G., Martins J.T., Lüdtke F.L., Geraldo A., Pereira A., Vicente A.A, & Vieira J.M. (2023). Chitosan Coating Functionalized with Flaxseed Oil and Green Tea Extract as a Bio-Based Solution for Beef Preservation. Foods, 12(7), 1447.

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Chitosan-Glycerol Biomaterial Preparation

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Chitosan was purchased from Golden-Shell Biochemical Co., Ltd. (Yuhuan, China) with high molecular weight and a degree of deacetylation of 95%. Glycerol 87% was obtained from Panreac (Barcelona, Spain). Tween 80 and l(␤)-lactic acid 90% were obtained from Acros Organics (Geel, Belgium).

Costa M.J., Cerqueira M.A., Ruiz H.A., Fougnies C., Richel A., Vicente A.A., Teixeira J.A, & Aguedo M. (2015). Use of wheat bran arabinoxylans in chitosan-based films: Effect on physicochemical properties. Industrial Crops & Products., 66.

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Characterization of Lignocellulosic Biomass

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Hard red winter wheat straw was collected from south-eastern Washington. The wheat straw sample was air-dried and milled to a specific size (mesh −40/+60), which was composed of 22.4 ± 0.1% lignin, 40.8 ± 0.6% cellulose, 29.2 ± 0.2% hemicellulose, and 3.4 ± 0.3% ash, and 3.2 ± 0.1% extractives. L(+)-Lactic acid (90% solution in water), ethanol (95%), choline chloride (99%), pyridine (99+%), and aniline (99+%) were purchased from Fisher Scientific. Pyrazole (>98%) was purchased from TCI America. Alkali Lignin (AL, CAS: 8068-05-1) and Technical Sodium Lignosulfonate (LS, CAS: 8061-51-6) samples were, respectively, purchased from Sigma-Aldrich and Spectrum Chemical. All chemicals were used as received.

Lin K.T., Wang C., Guo M.F., Aprà E., Ma R., Ragauskas A.J, & Zhang X. (2023). Lignin with controlled structural properties by N-heterocycle-based deep eutectic solvent extraction. Proceedings of the National Academy of Sciences of the United States of America, 120(32), e2307323120.

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