Cell lysates was separated by SDS–polyacrylamide gel (4–10%) electrophoresis, and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Massachusetts, USA). The membranes were then blocked with 5% skimmed milk and incubated overnight at 4 °C with the following primary detection antibodies: anti-CENPK (1:1000, Abcam, Cambridge, England), anti-C-MYC (1:1000, Abcam, England), anti-Cyclin D (1:1000, CST, Boston, USA), anti-Cyclin E (1:1000, Abcam, Cambridge, England), anti-Rb (1:1000, CST, Boston, USA), anti-p-Rb (1:1000, CST, Boston, USA), anti-GAPDH (1:10,000, Abcam, Cambridge, England), anti-JAK1 (1:1000, Abcam, Cambridge, England), anti-STAT3 (1:1000, Abcam, Cambridge, England), or anti-p-STAT3 (1:1000, Abcam, Cambridge, England). The species-matched secondary antibodies were then incubated for 1 h at room temperature and the proteins were detected using BeyoECLPlus (Beyotime, Jiangsu, China).
Anti cenpk
Manufactured by Abcam
Sourced in United Kingdom
Anti-CENPK is a primary antibody that recognizes the CENPK protein. CENPK is a component of the centromere-associated NDC80 complex, which plays a role in kinetochore-microtubule attachment and chromosome segregation during cell division.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti cenpk
1
Western Blot Analysis of Cell Cycle Markers
2
Western Blot Analysis of Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell lysates was separated by SDS-polyacrylamide gel (4%-10%) electrophoresis, and then transferred to polyvinylidene uoride (PVDF) membranes (Millipore, Massachusetts, USA). The membranes were then blocked with 5% skimmed milk and incubated overnight at 4°C with the following primary detection antibodies: anti-CENPK (1:1000, Abcam, Cambridge, England), anti-C-MYC (1:1000, Abcam, England), anti-Cyclin D (1:1000, CST, Boston, USA), anti-Cyclin E (1:1000, Abcam, Cambridge, England), anti-Rb (1:1000, CST, Boston, USA), anti-p-Rb (1:1000, CST, Boston, USA), anti-GAPDH (1:10000, Abcam, Cambridge, England), anti-JAK1 (1:1000, Abcam, Cambridge, England), anti-STAT3 (1:1000, Abcam, Cambridge, England), or anti-p-STAT3 (1:1000, Abcam, Cambridge, England). The species-matched secondary antibodies were then incubated for 1 h at room temperature and the proteins were detected using BeyoECLPlus (Beyotime, Jiangsu, China).
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