Plga resomer rg 503

Iron(iii) acetylacetonate, chloroform, oleic acid and oleylamine were purchased from Strem Chemicals, Inc. Postfach, Kehl, Germany. 1,2-Hexadecanediol and benzyl ether were obtained from Sigma-Aldrich, St. Louis, MO, USA. PLGA (Resomer RG 503, MW: 24 000–38 000 Da) was purchased from Evonik Industries, Germany. Tetrandrine (Tet, >98%) was obtained from Wuhan Dinghui Chemical Co., Ltd, China. Polyvinyl alcohol (PVA, Mowiol 4-88) was obtained from Kuraray Specialties Europe GmbH, Frankfurt, Germany. The PromoKine Annexin V-FITC Detection Kit was purchased from PromoCell GmbH, Heidelberg, Germany. BCA protein assay kits were purchased from BioRad, Hercules, USA. The antibodies for Cathepsin B, Bcl-2, Smac/Diablo, XIAP, Cytochrome C, Caspase 3, β-actin and GAPDH were obtained from Cell Signaling Technology, Danvers, MA, USA.

Porcine original mucus were obtained from intestine of freshly slaughtered pigs and mucus was gently scraped from the intestinal wall (Shenyang, Liaoning). In order to minimize the influence of mucus variability on the result, mucus sample was collected and stored at −20 °C before use to make sure the mucus sample used for each compound was from the same source [11] (link), [12] (link). Poloxamer 407 and 188 (P407, P188) were obtained as gifts from BASF (Germany). SDS was bought from Biotopped Co., Ltd.(China). PLGA (Resomer^® RG 503) was purchased from Evonik Industries (Germany). Poly(vinyl alcohol) (PVA 205) was obtained from Kuraray China Co., Ltd.(China). Coumarin 6 were purchased from J&K chemical Ltd.(China). Tween 80 was obtained from Tianjin Bodi Chemical Co., Ltd. (Tianjin, China). DAPI (4′,6-diamidino-2-phenylindole) staining solution and antifade mounting medium were bought from Beyotime Institute of Biotechnology (China).