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Mmesseage mmachine t7 kit

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The MMESSEAGE mMACHINE T7 kit is a laboratory tool used for the in vitro transcription of RNA from DNA templates. The kit contains the necessary reagents and components to facilitate the in vitro synthesis of RNA from a T7 promoter-driven DNA template.

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Lab products found in correlation

Megashortscript t7 high yield transcription kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

T7 mMachine kit T7 kits

2 protocols using mmesseage mmachine t7 kit

1

In vitro Transcription of BE3 Plasmids and sgRNAs

Cited 1 time
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In vitro transcription was performed as published previously (Luo et al. 2022 (link)). BE3 plasmids were linearized with NotI and underwent in vitro transcription using mMESSEAGE mMACHINE T7 kit (Invitrogen, Thermo Fisher Scientific) and finally purified by LiCl precipitation. sgRNAs were amplified and transcribed in vitro using MEGAshortscript T7 High Yield Transcription Kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions. Primers are listed in Supplementary Table 2. After transcription, sgRNAs were purified by ethanol precipitation.
Shi Y., Hu B., Wang Z., Wu X., Luo L., Li S., Wang S., Zhang K, & Wang H. (2023). Functional role of GATA3 and CDX2 in lineage specification during bovine early embryonic development. Reproduction (Cambridge, England), 165(3), 325-333.
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2

Plasmid Linearization and sgRNA Synthesis

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BE3 and ABE7.10 plasmids were purchased from Addgene (#73021 and #102919). After linearization with NotI, the plasmid underwent in vitro transcription using mMESSEAGE mMACHINE T7 kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and was purified by LiCl precipitation. sgRNAs were amplified and transcribed in vitro using MEGAshortscript T7 High Yield Transcription Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions. Primers are listed in S2 Table. After transcription, sgRNAs were purified by ethanol precipitation.
Luo L., Shi Y., Wang H., Wang Z., Dang Y., Li S., Wang S, & Zhang K. (2022). Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency. PLoS Genetics, 18(7), e1010307.
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