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Picoprobe acetyl coenzyme a assay kit

Manufactured by Abcam
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The PicoProbe Acetyl-coenzyme A assay kit is a laboratory tool designed to detect and measure the levels of acetyl-coenzyme A in various samples. The kit utilizes a fluorometric method to quantify acetyl-coenzyme A concentrations.

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Lab products found in correlation

Pyruvate assay kit, by Abcam (2 mentions) K607 lactate colorimetric fluorometric assay kit, by Abcam (2 mentions) Citrate assay kit, by Abcam (2 mentions) Nad nadh quantitation colorimetric kit, by Abcam (2 mentions)

2 protocols using picoprobe acetyl coenzyme a assay kit

1

Metabolic Profiling of Murine Macrophages

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Culture supernatant from BMDMs was collected as indicated in the experiments and the cytokine concentrations were measured by commercial ELISA kits according to the manufacturer’s instructions (BioVision). Lactate levels in media were quantified by using a lactate lit (K607 Lactate Colorimetric/Fluorometric Assay Kit, BioVision). The intracellular concentrations of acetyl-coA, citrate and pyruvate in BMDMs were detected by using the PicoProbe Acetyl-coenzyme A assay kit (ab87546, Abcam), the citrate assay kit (ab83396, Abcam) and the Pyruvate assay kit (K609, BioVision), respectively. For NAD and NADH measurement, total NAD and NADH levels were measured using a NAD/NADH Quantitation Colorimetric Kit (K337, BioVision). The ratios of NAD+ (NAD–NADH) to NADH were calculated. All experiments were performed at least three times and the data were normalized by the cell numbers or protein contents.
Liu P.S., Chen Y.T., Li X., Hsueh P.C., Tzeng S.F., Chen H., Shi P.Z., Xie X., Parik S., Planque M., Fendt S.M, & Ho P.C. (2023). CD40 signal rewires fatty acid and glutamine metabolism for stimulating macrophage anti-tumorigenic functions. Nature Immunology, 24(3), 452-462.
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2

Metabolic Profiling of Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Culture supernatant from BMDMs was collected as indicated in the experiments and the cytokine concentrations were measured by commercial ELISA kits according to the manufacturer’s instructions (BioVision). Lactate levels in media were quantified by using a lactate lit (K607 Lactate Colorimetric/Fluorometric Assay Kit, Biovision). The intracellular concentrations of acetyl-coA, citrate, and pyruvate in BMDMs were detected by using the PicoProbe Acetyl-coenzyme A assay kit (ab87546, Abcam), the citrate assay kit (ab83396, Abcam), the Pyruvate assay kit (K609, BioVision), respectively. For NAD and NADH measurement, total NAD and NADH levels were measured using a NAD/NADH Quantitation Colorimetric Kit (K337, Biovision). The ratios of NAD+ (NAD - NADH) to NADH were calculated. All experiments were performed at least three times and the data were normalized by the cell numbers or protein contents.
Liu P.S., Chen Y.T., Li X., Hsueh P.C., Tzeng S.F., Chen H., Shi P.Z., Xie X., Parik S., Planque M., Fendt S.M, & Ho P.C. (2023). CD40 signal rewires fatty acid and glutamine metabolism for stimulating macrophage anti-tumorigenic functions. Nature Immunology, 24(3), 452-462.
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