Mouse serum levels of cytokines/chemokines were measured using a Bio-Plex ProTM Mouse Cytokine 23-plex Assay Kit (Bio-Rad) on a Bio-Plex system following the manufacturer’s instructions.
Bio plex protm mouse cytokine 23 plex assay kit
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex Pro Mouse Cytokine 23-plex Assay Kit is a multiplex immunoassay designed to quantify the levels of 23 different cytokines in mouse biological samples. The kit utilizes magnetic bead-based technology to enable the simultaneous detection and measurement of multiple analytes in a single well. The assay is compatible with the Bio-Plex 200 and Bio-Plex MAGPIX systems.
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4 protocols using bio plex protm mouse cytokine 23 plex assay kit
1
Cytokine and Chemokine Profiling in Mouse Serum
2
Multiplexed Cytokine Profiling of Co-Cultured Cells
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FR cells-derived cytokines were searched for performed in the conditioned media of the co-cultured cells using the Bio-Plex ProTM Mouse Cytokine 23-plex Assay Kit (#M60-009RDPD, BioRad), according to manufacturers’ instructions. Samples were studied in triplicate in a Bio-Plex® 200 System (BioRad), and the attained results were analyzed using the Bio-Plex ManagerTM Software, Standard Edition (BioRad).
3
Multiplex Cytokine Measurement in Serum
4
Evaluating TMEV-Induced Cytokine Responses
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We used the serum collected before (baseline) and after injection (at 4 d.p.i. and 14 d.p.i.) to evaluate TMEV-induced immune responses. Immune response proteins were measured with Bio-Plex ProTM Mouse Cytokine 23-plex Assay kit (Bio-Rad, Hercules, CA, USA) to determine concentration levels of 23 cytokines and chemokines (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17α, IFN-γ, CCL11 [Eotaxin], G-CSF, GM-CSF, CXCL1 [KC {keratinocyte-derived chemokine}], CCL2 [MCP-1 {Monocyte Chemotactic Peptide 1}]), CCL3 [MIP-1α {Macrophage Inflammatory Protein 1α}], CCL4 [MIP-1β], CCL5 [RANTES], and TNF-α). Data were processed and analyzed using the Bio-Plex Manager software program (Bio-Rad version 4.1.1, Hercules, CA, USA).
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