Trace gc ms ultra system

In addition, parts of the collected samples were analyzed by GC-MS to investigate the secondary metabolite composition. A measure of 500 mg of dry lichen biomass was macerated in 10 mL methanol for 24 h at 300 rpm. The obtained extract was analyzed by a Trace GC-MS Ultra system with DSQII (Thermo Scientific, Waltham, MA, USA). A TriPlus autosampler was employed to inject 1 µL of sample volume in split mode onto a SGE BPX5 column (30 m, I.D 0.25 mm, film 0.25 µm); injector temperature was set to 280 °C. Initial oven temperature was kept at 50 °C for 2.5 min. The temperature was increased with a ramp rate of 10 °C/min to 320 °C with a final hold step for 3 min. The carrier gas in this study was helium with a flow rate of 0.8 mL/min and a split ratio of 8. The mass spectra and chromatograms were recorded at 70 eV (EI). Detection of masses took place between 50 m/z and 650 m/z in the positive mode [53 (link)]. Compounds were identified by spectral comparison with a NIST/EPA/NIH MS library version 2.0.

Fresh mature leaves were weighed in GC headspace vials and analyzed using a Trace GC-MS Ultra system with DSQII (Thermo Scientific, Waltham, MA, USA). Vials were incubated for 30 min at 100 °C and a TriPlus autosampler was used to inject 500 µL of the sample in split mode onto a SGE BPX5 column (30 m, I.D 0.25 mm, film 0.25 µm); an injector temperature of 280 °C was used. The initial oven temperature was kept at 50 °C for 2.5 min. The temperature was increased with a ramp rate of 10 °C/min to 320 °C with a final hold for 5 min. Helium was used as a carrier gas with a flow rate of 1.2 mL/min and a split ratio of 8. The MS chromatograms and spectra were recorded at 70 eV (EI). Masses were detected between 50 m/z and 650 m/z in the positive mode [69 (link)]. Samples were measured in biological triplicates and the area average was used to compare peaks. Compounds were identified by spectral comparison with a NIST/EPA/NIH MS library version 2.0. To provide insight into the differentiation between plant samples a PCA was conducted.