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Female c57bl 6 b6 mice

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Female C57BL/6 (B6) mice are a commonly used inbred strain of laboratory mice. They are widely utilized in biomedical research due to their well-characterized genetics and physiology.

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Lab products found in correlation

Bcl6 cd4 cre mice, by Jackson ImmunoResearch (2 mentions) Bcl6fl fl cd4 cre0 mice, by Jackson ImmunoResearch (2 mentions) Il 21r mice, by Jackson ImmunoResearch (2 mentions) Ain 93g, by DooYeol Biotech (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Mem amino acids, by Thermo Fisher Scientific (1 mentions) Dmem f12 hepes, by Thermo Fisher Scientific (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)

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C57BL/6 mice (3627 citations) C57BL/6 (788 citations) C57BL/6 (B6) mice (66 citations) C57BL/6 females (30 citations) C57BL/6 (B6) (14 citations) C57BL/6 and B6-Ly5.2 female mice (3 citations)

9 protocols using female c57bl 6 b6 mice

1

Dietary Fiber Modulation in C57BL/6 Mice

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Six- to eight-week-old C57BL/6 (B6) female mice were purchased from Charles River Laboratories (OrientBio). All mice were maintained under specific pathogen-free (SPF) conditions and each cage contained 3–5 mice. Mice were fed a normal chow diet (NCD) (6.0% crude fiber [Purina 5057], Purina), LCD (0.3% cellulose in AIN-93G, DooYeol Biotech), or HCD (30.0% cellulose in AIN-93G, DooYeol Biotech) (Figure S1).
Kim Y., Hwang S.W., Kim S., Lee Y.S., Kim T.Y., Lee S.H., Kim S.J., Yoo H.J., Kim E.N, & Kweon M.N. (2020). Dietary cellulose prevents gut inflammation by modulating lipid metabolism and gut microbiota. Gut Microbes, 11(4), 944-961.
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2

Mice Sourcing and Handling for Vision Research

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C57BL/6 (B6) female mice were purchased from Charles River Laboratories, housed in a SPF-free environment at Duke, and managed according to the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research.
Singh P.P., Yu C., Mathew R., Perez V.L, & Saban D.R. (2021). Meibomian gland dysfunction is suppressed via selective inhibition of immune responses by topical LFA-1/ICAM antagonism with lifitegrast in the allergic eye disease (AED) model. The ocular surface, 21, 271-278.
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3

Mouse Husbandry and Breeding Protocols

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C57BL/6 (B6) female mice were purchased from Charles River Laboratories or the National Cancer Institute or subsequently bred and housed at Yale University. Rag2−/− mice were bred and housed at Yale University. All animal procedures were performed in compliance with Yale Institutional Animal Care and Use Committee protocols.
Khoury-Hanold W., Yordy B., Kong P., Kong Y., Ge W., Szigeti-Buck K., Ralevski A., Horvath T.L, & Iwasaki A. (2016). Viral spread to enteric neurons links genital HSV-1 infection to toxic megacolon and lethality. Cell host & microbe, 19(6), 788-799.
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4

Conditional Knockout Mice for Bcl6 and IL-21R

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Female C57BL/6 (B6) mice were purchased from Charles River Laboratories (Bethesda, MD). Bcl6+/+;Cd4-Cre+ mice, Bcl6fl/fl;Cd4-Cre0 mice, and IL-21R−/− mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Bcl6+/+;Cd4-Cre+ mice and Bcl6fl/fl; Cd4-Cre0 mice were bred for two generations to obtain Bcl6fl/fl; Cd4-Cre+ mice that lacked Bcl-6 expression in CD4+ cells and are referred to in the article as “ablated mice.” Littermates (Bcl6+/+; Cd4-Cre)+ that lacked floxed Bcl-6 but expressed Cre recombinase and those that expressed the floxed allele but lacked Cre expression (Bcl6fl/fl;Cd4-Cre0) were used as controls. Genotyping was carried out according to the protocol provided by The Jackson Laboratory for mouse stock numbers 023727 (Bcl6fl/fl) and 022071 (Cd4-Cre+). Validation of genotype and phenotype was as reported by us previously (13 (link)).
Lang G.A., Amani S.A., Quinn J.L., Axtell R.C, & Lang M.L. (2019). Immunization-Expanded NKT Follicular Helper Cells Drive IgG1 Isotype Switch against an Exogenous T-Independent Polysaccharide but Do Not Promote Recall Responses. ImmunoHorizons, 3(3), 88-93.
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5

Murine B6 YUMM1.7 Tumor Model

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Female C57BL/6 (B6) mice (6 to 8 weeks old) were purchased from Charles River. Mice were housed in specific pathogen–free conditions. All procedures were conducted in accordance with the UK Home Office Animals (Scientific Procedure) Act of 1986 and were approved by the UCL Animal Welfare and Ethical Review Body (PP4506002). Murine B6 YUMM1.7 tumor cells [American Type Culture Collection (ATCC) CRL-3362] were grown in Dulbecco’s modified Eagle’s medium (DMEM)–F12, Hepes (Thermo Fisher Scientific, no. 11330032) supplemented with 10% heat-inactivated fetal bovine serum (iFBS, Merck, F7524-500ML), β-mercaptoethanol, 1% penicillin, streptomycin, l-glutamine, and nonessential minimal essential medium (MEM) amino acids (all Thermo Fisher Scientific or Sigma-Aldrich) according to ATCC recommendations. Cells were harvested with 0.05% EDTA (Thermo Fisher Scientific, no. 25300062) and counted before injection. Mice received 5 × 105 tumor cells injected intradermally into the shaved flank. Recipients were cohoused where possible.
Howell R., Davies J., Clarke M.A., Appios A., Mesquita I., Jayal Y., Ringham-Terry B., Boned Del Rio I., Fisher J, & Bennett C.L. (2023). Localized immune surveillance of primary melanoma in the skin deciphered through executable modeling. Science Advances, 9(15), eadd1992.
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6

Conditional Knockout Mice for Bcl6 and IL-21R

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Female C57BL/6 (B6) mice were purchased from Charles River Laboratories (Bethesda, MD). Bcl6+/+;Cd4-Cre+ mice, Bcl6fl/fl;Cd4-Cre0 mice, and IL-21R−/− mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Bcl6+/+;Cd4-Cre+ mice and Bcl6fl/fl; Cd4-Cre0 mice were bred for two generations to obtain Bcl6fl/fl; Cd4-Cre+ mice that lacked Bcl-6 expression in CD4+ cells and are referred to in the article as “ablated mice.” Littermates (Bcl6+/+; Cd4-Cre)+ that lacked floxed Bcl-6 but expressed Cre recombinase and those that expressed the floxed allele but lacked Cre expression (Bcl6fl/fl;Cd4-Cre0) were used as controls. Genotyping was carried out according to the protocol provided by The Jackson Laboratory for mouse stock numbers 023727 (Bcl6fl/fl) and 022071 (Cd4-Cre+). Validation of genotype and phenotype was as reported by us previously (13 (link)).
Lang G.A., Amani S.A., Quinn J.L., Axtell R.C, & Lang M.L. (2019). Immunization-Expanded NKT Follicular Helper Cells Drive IgG1 Isotype Switch against an Exogenous T-Independent Polysaccharide but Do Not Promote Recall Responses. ImmunoHorizons, 3(3), 88-93.
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7

Mouse Handling and Anesthesia Protocol

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Female C57BL/6 (B6) mice aged 8–12 weeks were purchased from Charles River Laboratories. CD11c-eGFP/DTR mice also 8–12 weeks were gifted from the labs of Soman Abraham (Department of Pathology, Duke University) and Mari Shinohara (Department of Immunology, Duke University), which were originally purchased from Jackson Laboratory (stock no. 004509). Mice were housed in a specific pathogen-free environment at the Duke Eye Center animal facility. Anesthesia was used for all surgical procedures with i.p.-administered ketamine/xylazine suspensions (120 and 20 mg/kg, respectively).
Ahadome S.D., Mathew R., Reyes N.J., Mettu P.S., Cousins S.W., Calder V.L, & Saban D.R. (2016). Classical dendritic cells mediate fibrosis directly via the retinoic acid pathway in severe eye allergy. JCI Insight, 1(12), e87012.
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8

Adult Female C57BL/6 Mouse Husbandry

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Female C57BL/6 (B6) mice were purchased from Charles River Canada Inc. (St. Constant, Quebec) and housed in our institutional barrier facility. Closely age-matched, adult mice were used following an animal use protocol approved by the Western University Animal Use Subcommittee and the Canadian Council on Animal Care guidelines.
Memarnejadian A., Meilleur C.E., Shaler C.R., Khazaie K., Bennink J.R., Schell T.D, & Haeryfar S.M. (2017). PD-1 blockade promotes epitope spreading in anticancer CD8+ T cell responses by preventing fratricidal death of subdominant clones to relieve immunodomination. Journal of immunology (Baltimore, Md. : 1950), 199(9), 3348-3359.
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9

NKT Cell Deficient Mouse Protocol

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Female C57BL/6 (B6) mice were acquired from Charles River Laboratories. Female Cd1d−/− mice (NKT cell‐deficient mice) on a B6 background were acquired from the Jackson Laboratory. All mice were maintained in specific pathogen‐free conditions.
Naruo M., Negishi Y., Okuda T., Katsuyama M., Okazaki K, & Morita R. (2021). Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity. Immunity, Inflammation and Disease, 9(4), 1370-1382.
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