The levels of IL-10, IL-1β, IFN-γ, and TNF-α in supernatants from uninfected and VSV-infected leukocytes were detected using enzyme-linked immunosorbent assay (BD OptEIA TM human IL-10, IL-1β, IFN-γ, or TNF-α enzyme-linked immunosorbent assay (ELISA) set, BD Biosciences). The optical density was measured at 450 nm with λ correction of 570 nm using a Multiskan RC spectrophotometric reader (Thermo Labsystems, USA). Cytokine concentrations were expressed in picograms per ml.
Multiskan rc spectrophotometric reader
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Multiskan RC spectrophotometric reader is a laboratory instrument designed for absorbance measurements. It is capable of performing photometric analyses across a wide range of applications.
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Lab products found in correlation
4 protocols using multiskan rc spectrophotometric reader
1
Cytokine Profiling of VSV-Infected Leukocytes
2
Cytokine Quantification by ELISA
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Cytokine levels were determined in the supernatants using ELISA kits (Biomedical Laboratories, USA; BD Biosciences, USA) for human IL-1β, IL-6, IL-10, TNF-α according to the producers' instructions. The optical density was measured at 450 nm using a Multiskan RC spectrophotometric reader (Thermo Labsystems, USA). Cytokine concentrations were expressed in pg/ml.
3
Cytokine Profiling of VSV-infected Leukocytes
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The levels of IL-1β, IL-10, IL-15, IFN-α, IFN-γ, and TNF-α in supernatants from uninfected and VSV-infected leukocytes were detected using enzyme-linked immunosorbent assays (BD OptEIA TM human IL-1β, IL-10, IL-15, IFN-γ,TNF-α ELISA set, BD Biosciences; IFN-α Human ELISA Kit, Thermo Fisher, Carlsbad, CA, USA). The optical density was measured at 450 nm with λ correction 570 nm using a Multiskan RC spectrophotometric reader (Thermo Labsystems, Philadelphia, PA, USA). Cytokine concentrations were expressed in pg/mL.
4
Quantifying Cytokine Levels by ELISA
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Cytokine levels were determined in supernatants using ELISA kits for human TNF-α and IL-6 according to the manufacturer’s instructions. The optical density was measured at λ = 450 nm using a Multiskan RC spectrophotometric reader (Thermo Labsystems, Waltham, MA, USA).
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