Dulbecco modified eagle medium (dmem)

Manufactured by Capricorn

Sourced in Germany, United States

DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium commonly used in biomedical research and cell biology applications. It provides essential nutrients, vitamins, and amino acids required for the growth and maintenance of various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Capricorn (20 mentions) Penicillin streptomycin, by Capricorn (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Trypsin edta, by Thermo Fisher Scientific (4 mentions) L glutamine, by Capricorn (3 mentions) Fetal bovine serum (fbs), by Merck Group (3 mentions) L glutamine, by Merck Group (3 mentions) Penicillin streptomycin solution, by Capricorn (2 mentions) L glutamine media, by Capricorn (2 mentions) Trypsin edta, by Capricorn (2 mentions) Poly l lysine hydrobromide, by Merck Group (2 mentions) Plasmocin, by InvivoGen (2 mentions) Mycoalert plus mycoplasma detection kit, by Lonza (2 mentions) Ultrapure agarose, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Capricorn (2 mentions) Fetal calf serum (fcs), by Capricorn (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)

51 protocols using dulbecco modified eagle medium (dmem)

Isolation and Serum Starvation of Neonatal Fibroblasts

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fibroblasts were isolated from fresh foreskin prepared from children aged 1.5–3 months who underwent routine circumcision at Amirkola children's hospital in Iran, as described above. Isolated fibroblasts were then cultured routinely in high glucose (4.5 g/L) DMEM (Capricorn scientific) supplemented with 10% (v/v) FBS (Capricorn scientific), 100 μg/ml penicillin-streptomycin (PAA, cat: p11-010) at 37 °C in a humidified 5% CO₂ atmosphere and reached passages 3. Due to the cultural realization that considers the foreskin as a throwaway tissue, the ethic committee of Babol University of Medical Sciences waived the need for a written consent for foreskin preparation.
The serum starvation protocol was used as previously described with minor modification [30 (link)]. To this end, 3 × 10⁵ neonatal dermal fibroblasts cells at passage 3 were cultured in five 75 ml-flasks (SPL) with DMEM +10% FBS (Capricorn scientific) at 37 °C in humidified 5% CO₂ atmosphere. After 48 h the cell culture supernatant was removed and cells were washed twice PBS followed by wash with DMEM without FBS (Capricorn scientific). Then, cells were re-suspended in DMEM (Capricorn scientific) or DMEM+10% FBS (all Capricorn scientific) and seeded in separate flasks and cultured for 72 h. Then, fibroblasts in the flask were detached by incubation with 0.25% trypsin/EDTA for 5 min and were washed for further use.

Jafari N., Gheitasi R., Khorasani H.R., Golpour M., Mehri M., Nayeri K., Pourbagher R., Mostafazadeh M., Kalali B, & Mostafazadeh A. (2023). Proteome analysis, bioinformatic prediction and experimental evidence revealed immune response down-regulation function for serum-starved human fibroblasts. Heliyon, 9(9), e19238.

+ Open protocol

+ Expand

Isolation and Starvation of Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fibroblasts were isolated from a fresh foreskin, as described previously [28 (link)]. The isolated cells were cultured in DMEM (Capricorn scientific, Ebsdorfergrund, Germany) supplemented with 10% fetal bovine serum (FBS) (Capricorn scientific, Ebsdorfergrund, Germany) until passage three. Then at 80% confluency in a 75 cm² cell culture flask (SPL life sciences, Pocheon, Korea), cells were washed twice with phosphate-buffered saline (PBS), and serum starvation conditions were started for 16 h in serum-free, glucose-containing DMEM (Capricorn scientific, Ebsdorfergrund, Germany) without antibiotics. Cell culture supernatants were collected, and stored at −20 °C for further analysis. The collected conditioned supernatant has been named as 16h-starved fibroblast culture supernatant (16h-SFS). The current protocol has been approved by the local ethical committee at Babol University of Medical Sciences (reference number: IR. MUBABOL.HRI.REC.1398.333).

+ Open protocol

+ Expand

Non-Small Cell Lung Cancer Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human non-small lung cancer A549 cells, H1299 cells were purchased from American Type Culture Collection (Rockville, MD, USA). A549 cells were grown in Dulbecco Modified Eagle Medium (Capricorn Scientific, Ebsdorfergrund, Germany) supplemented with 10% fetal bovine serum (Capricorn Scientific, Ebsdorfergrund, Germany) and 1% penicillin/streptomycin solution (Capricorn Scientific, Ebsdorfergrund, Germany). H1299 cells were grown in Roswell Park Memorial Institute with l-glutamine media (RPMI, Capricorn Scientific, Ebsdorfergrund, Germany). The cells were maintained in the monolayer at 37 °C in a humidified atmosphere with 5% CO₂.
Ferrostatin-1 (Cat# S7243), RSL3 (Cat# S8155) were purchased from Selleck Chemicals (Houston, USA). 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Cat# M1415) was purchased from Duchefa Biochemie (Harrlem, Netherlands). PrimeScript RT Master mix (Cat# RR036) and TB Green (Cat# RR430) were purchased from TAKARA (Shiga, Japan). The primary antibodies against GAPDH (Cat# 5174) and SLC7A11 (Cat# 12691) were purchased Cell Signaling Technology (Massachusetts, USA). NRF2 (Cat# 16396), FSP1 (Cat# 20886) and ACSL4 (Cat# 22401) were purchased from Proteintech (Rosemont, USA). 4-hydroxynonenal (4-HNE, Cat# ab46546) was purchased from Abcam (Cambridge, UK).

Jo A., Bae J.H., Yoon Y.J., Chung T.H., Lee E.W., Kim Y.H., Joh H.M, & Chung J.W. (2022). Plasma-activated medium induces ferroptosis by depleting FSP1 in human lung cancer cells. Cell Death & Disease, 13(3), 212.

+ Open protocol

+ Expand

Cell Culture Conditions for HeLa, A549, T24, and H1299

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa, A549 and T24 cells were grown in Dulbecco Modified Eagle Medium (Capricorn Scientific, Ebsdorfergrund, Germany) and H1299 were incubated in Roswell Park Memorial Institute with L-glutamine media (RPMI, Capricorn Scientific, Ebsdorfergrund, Germany). The media were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Capricorn, Scientific, Ebsdorfergrund, Germany). The cells were incubated in a humidified atmosphere with 5% CO₂ at 37°C.

Jo A., Joh H.M., Bae J.H., Kim S.J., Chung T.H, & Chung J.W. (2022). Plasma activated medium prepared by a bipolar microsecond-pulsed atmospheric pressure plasma jet array induces mitochondria-mediated apoptosis in human cervical cancer cells. PLoS ONE, 17(8), e0272805.

+ Open protocol

+ Expand

Cell Culture Conditions for Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The T-24, HT29, and DLD-1 cells were grown in DMEM (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and the MDA-MB-231, RKO, and HCT116 cells were incubated in Roswell Park Memorial Institute with L-glutamine media (Capricorn Scientific GmbH, Ebsdorfergrund, Germany). The media were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Capricorn Scientific GmbH, Ebsdorfergrund, Germany). The T-24R cell line was provided by Professor Sun-Hee Leem (Dong-A University, Busan, Republic of Korea). The cells were incubated in a humidified atmosphere with 5% CO₂ at 37 °C.

Jo A., Joh H.M., Bae J.H., Kim S.J., Chung J.W, & Chung T.H. (2024). Plasma-Activated Media Produced by a Microwave-Excited Atmospheric Pressure Plasma Jet Is Effective against Cisplatin-Resistant Human Bladder Cancer Cells In Vitro. International Journal of Molecular Sciences, 25(2), 1249.

+ Open protocol

+ Expand

Erastin-induced cell death in HT22 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HT22 cells (kindly provided by David Schubert, Cellular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA) were grown in Dulbecco’s modified Eagle medium (DMEM, Capricorn Scientific GmbH, Ebsdorfergrund, Germany) supplemented with 10% heat-inactivated fetal calf serum (Merck KGaA, Darmstadt, Germany), 100 U/mL penicillin, 100 mg/mL streptomycin (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and 2 mM L-glutamine (Merck KGaA, Darmstadt, Germany). To induce cell death, erastin (Calbiochem^®, Merck KGaA, Darmstadt, Germany) was added to the medium for the indicated amount of time (8–16 h).

Rabenau M., Dillberger B., Günther M., Krippner S., Butterweck V., Boonen G., Drewe J., Eckert G.P, & Culmsee C. (2021). Cimicifuga racemosa Extract Ze 450 Re-Balances Energy Metabolism and Promotes Longevity. Antioxidants, 10(9), 1432.

+ Open protocol

+ Expand

Cell Viability Assay with CellTiter-Glo

Check if the same lab product or an alternative is used in the 5 most similar protocols

The assays were performed using the CellTiter-Glo assay from Promega (Madison, AL, USA). The assay detects cellular adenosine triphosphate (ATP) content with the amount of ATP being directly proportional to the number of cells present. The A549 cells were obtained from DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) and W1-38 cells were obtained from ATCC (ATCC^® CCL-75™) and were grown in Dulbecco's Modified Eagle Medium (DMEM) from Capricorn Scientific GmbH, Ebsdorfergrund, Germany with fetal bovine serum (FBS) (10% v/v) from Capricorn Scientific GmbH, Ebsdorfergrund, Germany, streptomycin (100 μg/mL) and penicillin G (100 U/mL) [45 (link)].

Di Pisa F., Landi G., Dello Iacono L., Pozzi C., Borsari C., Ferrari S., Santucci M., Santarem N., Cordeiro-da-Silva A., Moraes C.B., Alcantara L.M., Fontana V., Freitas-Junior L.H., Gul S., Kuzikov M., Behrens B., Pöhner I., Wade R.C., Costi M.P, & Mangani S. (2017). Chroman-4-One Derivatives Targeting Pteridine Reductase 1 and Showing Anti-Parasitic Activity. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(3), 426.

+ Open protocol

+ Expand

Cellular Signaling Pathway Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

DMEM, FBS, penicillin/streptomycin, L-glutamine, and trypsin-EDTA were purchased from Capricorn Scientific, NEBuilder HiFi DNA Assembly kit and Q5 Polymerase from New England Biolabs, Effectene Transfection Reagent from Qiagen, a saponin from AppliChem, poly-L-lysine hydrobromide, GTPγS, acetylcholine, and carbachol from Sigma-Aldrich, histamine from Alfa Aesar and pertussis toxin from Merck.

Jelinek V., Mösslein N, & Bünemann M. (2021). Structures in G proteins important for subtype selective receptor binding and subsequent activation. Communications Biology, 4, 635.

+ Open protocol

+ Expand

Splenocyte Isolation and Flow Cytometry

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spleen cells were isolated at 14 days post infection (dpi). Homogenized single-cell suspensions were prepared in 6 mL of DMEM (Capricorn Scientific, Ebsdorfergrund, Hessen, Germany) supplemented with 10% FBS (Atlas Biologicals, Fort Collins, CO, USA) and 1% penicillin/streptomycin using gentleMACS™ Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were passed through a 70-µm cell strainer (SPL Life Sciences, Gyeonggi-do, South Korea), and centrifuged at 314× g for 7 min at 4 °C, followed by re-suspension and incubation in RBC lysis buffer (BioLegend, San Diego, CA, USA) at 4 °C for 5 min. After washing (314× g 7 min at 4 °C), cells were kept on ice in FACSFlow sheath fluid (BD Biosciences, San Jose, CA, USA) containing 0.05% FBS (Atlas Biologicals, USA) and Fc block (CD16/CD32 Fcγ III/II, BioLegend, San Diego, CA, USA) (1/1000 dilution) for 30 min in the dark at 4 °C. Next, 10⁵ cells per sample were incubated for 30 min in the dark at 4 °C, with antibody cocktails specific for different splenocytes populations, followed by flow cytometry analysis using a BD Accuri™ C6 Plus flow cytometer (BD Biosciences, San Jose, CA, USA). The gating strategies used have previously been published [84 (link)], and the percentage of each population was determined by dividing the number of events within a specific gate, by the total number of events within live gate.

Magez S., Li Z., Nguyen H.T., Pinto Torres J.E., Van Wielendaele P., Radwanska M., Began J., Zoll S, & Sterckx Y.G. (2021). The History of Anti-Trypanosome Vaccine Development Shows That Highly Immunogenic and Exposed Pathogen-Derived Antigens Are Not Necessarily Good Target Candidates: Enolase and ISG75 as Examples. Pathogens, 10(8), 1050.

+ Open protocol

+ Expand

Heat Shock Response in HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa (female; ATCC), HEK293T (fetal; ATCC), HEK293-EBNA (fetal; ATCC) and HEK293FT cells (fetal; ATCC) were maintained in Dulbecco's modified Eagle's medium (DMEM; Capricorn Scientific) supplemented with 10% fetal bovine serum (Capricorn Scientific) and 1% penicillin/streptomycin (Capricorn Scientific). To prevent mycoplasma contamination, all cultured cells were regularly treated with Plasmocin™ (Invivogen) and analyzed using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
For heat shock experiments, HeLa cells were subjected to heat shock treatment by incubating at 42°C for 30 min and recovery at 37°C for different periods.

Park J., Chang J., Hwang H.J., Jeong K., Lee H.J., Ha H., Park Y., Lim C., Woo J.S, & Kim Y.K. (2021). The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins. Nucleic Acids Research, 49(21), 12517-12534.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!