Sodium phosphate dibasic dehydrate

Dimethyl sulfoxide (DMSO) (purity: ≥99.7%) and acetonitrile (gradient grade for liquid chromatography, LiChrosolv^®), sodium phosphate dibasic dehydrate and sodium phosphate monobasic monohydrate were purchased from Sigma-Aldrich (Steinheim, Germany). Ultrapure water, obtained with the Millipore Direct-Q 3 UV Water Purification System (Millipore Corporation, Bedford, MA, USA), was utilized for purification of the water used for preparation of the buffer mobile phase.

Alginic acid sodium salt from brown algae (BioReagent, suitable for immobilization of microorganisms; 71238), calcium chloride (CaCl₂; C1016), albumin from bovine serum (BSA; A2153), sodium citrate dihydrate (C₆H₅Na₃O₇∙2H₂O; W302600), sodium chloride (NaCl; 746398), sodium hydroxide (NaOH; S5881), potassium hydrogen phthalate (C₈H₅KO₄; P1088), barium chloride (BaCl₂; B0750), sodium phosphate monobasic dihydrate (NaH₂PO₄∙2H₂O; 71505) and sodium phosphate dibasic dehydrate (Na₂HPO₄∙2H₂O; 71643) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hydrochloric acid (1N) was obtained from Merck KGaA (Darmstadt, Germany). For protein quantification, a bicinchoninic acid assay (BCA) kit (Visual Protein, Energenesis Biomedical Co., LTD., Taiwan) was used.

Chlorhexidine digluconate (20%,) polyvinylpyrrolidone (PVP)–iodine complex, sodium phosphate dibasic dehydrate, citric acid, isopropyl, polysorbate 80, catalase, hydrogen peroxide, bovine serum albumin, and iodine were obtained from Sigma Aldrich (USA). Malt extract agar (MEA) and tryptic soy agar (TSA) were purchased from Oxoid (UK). Maximum recovery diluent, lecithin, and defibrinated sheep blood were obtained from Merck, Alfa Aesar, and Thermo Fisher Scientific, respectively.

Absolute ethanol and sodium dihydrogen phosphate dihydrate extra pure were obtained from Riedel-de Haën (Seelze, Germany). Muller–Hilton medium was purchased from Biokar Diagnostic (France). Acetone, N-hexane, ethyl acetate, methanol, acetonitrile, trichloroacetic acid, and dimethyl sulfoxide were purchased from Merck (Darmstadt, Germany). Acetylcholinesterase, Ellman reagent, acetylthiocholine iodide, 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB), HEPES buffer, tacrine, 2,2-diphenyl-l-picrylhydrazyl, Dulbecco’s Modified Eagle’s medium (DMEM), fetal bovine serum, penicillin–streptomycin solution, hydrogen peroxide, trypsin, phosphate buffer saline, thiazolyl blue tetrazolium bromide, rosmarinic acid (RA), propylene glycol, and sodium phosphate dibasic dehydrate were obtained from Sigma-Aldrich (Darmstadt, Germany). Sodium chloride was supplied by José M. Vaz Pereira (Lisbon, Portugal). Benzophenone-4 was purchased from Mapric (São Paulo, Brazil). All other chemicals were of HPLC analytical grade, and they were used as received. Parvifloron D (ParvD) was isolated according to the method described by [16 (link)], and its NMR spectroscopic characterization is provided in the Supplementary Materials.

2,2-Diphenyl-1-picrylhydrazyl (DPPH), quercetin, DL-glyceraldehyde (dimer), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), glacial acetic acid, sodium phosphate dibasic dodecahydrate, trifluoroacetic acid, potassium phosphate monobasic, ethanol (99.8%), 1-propanol (99.7%), rosmarinic acid (97%), and sodium phosphate dibasic dehydrate were procured from Sigma-Aldrich Chemical Co. (St. Louis, MI, USA). An Amberlite^® IR-120 (H⁺ form) ion exchanger and ammonium sulfate were purchased from Merck KGaA Co. (Darmstadt, Germany). A 3A molecular sieve was purchased from Consolidated Chemical & Solvents LLC. (Quakertown, PA, USA). The other organic solvents were purchased from J. T. Baker Co. (Phillipsburg, NJ, USA), including HPLC grade for HPLC and preparative HPLC (Pre-HPLC) assays and analytical grade for extraction, fractionation, and HSCCC separations. The ultrapure water used in this study was produced using a Milli-Q water purification system (Millipore Co., Bedford, MA, USA).
The aerial parts of L. meyenii were collected from Lima, and the specimen was authenticated by Paul H. Gonzales Arce (P.H.G.A.). The dried material was placed at the Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University.

Cellulosic woven material such as viscose fabric 40 g/m² was primarily used and most of the evaluation of different functional properties was carried out on viscose fabric, however, cotton fabric of 70 g/m² was also studied. Prior to dyeing, both viscose and cotton fabric samples were cleaned using non-ionic detergent with subsequent hot and cold-water wash treatment.
All chemicals such as riboflavin, riboflavin 5′-monophosphate sodium salt hydrate widely known as flavin mononucleotide (FMN), sodium phosphate monobasic dehydrate and sodium phosphate dibasic dehydrate were purchased from Sigma Aldrich and used as received without any further pre-treatment.