Om d e m5 mark 3

Following the intervention as needed, MCF-7 and MDA-MB-231 cells (1 × 10³ cells/well) were seeded in six-well plates at 37°C and 5% CO₂ for 14 days, and the colonies formed were fixed with 4% paraformaldehyde (P1110; Solarbio Lifesciences, China) for 15 min and stained with crystal violet (C8470; Solarbio Lifesciences, China) for 30 min. All colonies formed were then observed and photographed using a digital camera (OM-D E-M5 Mark III; Olympus, Tokyo, Japan). The colony formation rates of cells in each group were calculated by the software SigmaPlot 12.0 (Systat Software, Inc., San Jose, CA, USA).

Provannid snails were observed and dissected under an Olympus SZX7 dissecting microscope and photographs were taken using a digital single reflex camera (Olympus OM-D E-M5 Mark III) mounted on the trinocular. For specimen photos, several photos taken at different focus levels were stacked automatically using Adobe Photoshop 2022 software. Shell height (SH), shell width (SW), aperture height (AH), and aperture width (AW) were measured using digital Vernier callipers, with the values rounded up to the nearest 0.1 mm. In specimens with a damaged aperture, only SH and SW were taken.