Fitc conjugated goat anti mouse igg antibody

The mouse anti-FLAG (Sigma-Aldrich, USA), mouse anti-Myc (Sigma-Aldrich, USA), rabbit anti-ATP6V1A (Sigma-Aldrich, USA), mouse anti-β-actin (Proteintech, China), mouse anti-HA epitope tag (Proteintech, China), mouse anti-GST (Sigma-Aldrich, USA), mouse anti-His epitope tag (Proteintech, China), rabbit IgG (Solarbio, China) and the secondary antibodies (HRP-conjugated anti-mouse IgG antibody [Sigma-Aldrich, USA], HRP-conjugated anti-rabbit IgG antibody [Genescript, China], FITC-conjugated goat anti-mouse IgG antibody [ZSGB. Bio, China], Alexa Fluor 647-labeled goat anti-rabbit IgG (H + L) [Beyotime, China]) and gold-labeled goat anti-mouse IgG antibody (Sigma-Aldrich, USA) were purchased from the indicated vendors. Murine sera against M, P, or G protein were produced in our laboratory.

An indirect IFA was used to detect sigma C protein expression in Sf9 cells. Briefly, the cells were fixed in cold methanol: acetone (1: 1, v: v) at −20 °C for 30 min. After three times washed with PBS, cells were incubated at 37 °C for 1 h with mouse antiserum specific for sigma C protein (prepared from Balb/c mouse vaccinated three times with the recombinant NDRV sigma C) diluted 1:500 in PBS. After washing with PBS three times, cells were incubated at 37 °C for 1 h with FITC-conjugated goat anti-mouse IgG antibody (1: 100 dilution with 0.1% Evans blue solution; ZSbio, China). Finaly, the stained cells washed three times with PBS were observed under a fluorescence microscope equipped with a video documentation system (Nikon, Japan).