All mice were on C57BL/6 background. WT mice (C57BL/6, Strain #002014), OTII mice (Strain #004194), and KitWSH/WSH (C57BL/6, Strain #005051) were purchased from the Jackson Laboratory. Il9r−/− mice (C57BL/6) were a gift from Dr. Jean-Christophe Renauld61 (link). Il9−/− mice (C57BL/6) were provided by Drs. Andrew McKenzie62 (link), Alexander Kirsch, and Sophie Paczesny. Il9fl/fl mice were generated by Jackson Laboratories using Cas9-mediated targeting as characterized in Ulrich & Kharwadkar et al40 (link). Experiments were performed using 6–16-week-old female and male mice with no observed differences between sexes. All mice were maintained in SPF animal facilities (ambient temperature 70–72° F, humidity 50%, light/dark cycle 12/12 hour). All experiments were performed with the approval of the Indiana University Institutional Animal Care and Use Committee.
Kitw sh w sh
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
KitW-sh/W-sh is a laboratory equipment product designed for research purposes. It serves as a tool for researchers to conduct studies, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6, by Jackson ImmunoResearch (3 mentions)
Il9fl fl mice, by Jackson ImmunoResearch (2 mentions)
Ot 2 mice, by Jackson ImmunoResearch (2 mentions)
Balb c mice, by Jackson ImmunoResearch (1 mentions)
Interleukin 6 (il 6), by Jackson ImmunoResearch (1 mentions)
Cpa3 cre, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Il33 mice, by Amgen (1 mentions)
Cd40l, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
7 protocols using kitw sh w sh
1
Genetic Mouse Models for Immunological Research
2
Mouse Strains for Immunological Studies
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C57BL/6, KitW-sh/W-sh, and BALB/C mice were purchased from The Jackson Laboratory. DCIR–/– mice were provided by Bernd Lepenies at The University of Veterinary Medicine Hannover (Hannover, Germany) and were bred in-house. CaMKII MM-VVδ mice were provided by Mark Anderson at Johns Hopkins University (53 (link)). IgE-KO mice were provided by Hans C. Oettgen at the Boston Children’s Hospital in Harvard Medical School (Boston, Massachusetts, USA) (86 (link)). All experiments were conducted using age- and sex-matched 6- to 12-week-old male and female mice. All mouse strains were bred and maintained under the same specific pathogen–free conditions at an American Association for the Accreditation of Laboratory Animal Care–accredited (AAALAC-accredited) animal facility at Johns Hopkins University and housed according to procedures described in the Guide for the Care and Use of Laboratory Animals (National Academies Press, 2011).
3
Rodent Models of Hypertension and Inflammation
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Experiments were performed using male rodents; these included spontaneously hypertensive rats (SHR) and the normotensive control wistar kyoto (WKY) rat, as well as wild type, Tac1−/−, Nk-1r−/−, tumor necrosis factor receptor I (TnfrI−/−), and B6.Cg-KitW-sh/HNihrJaeBsmGlliJ (Kitw-sh/w-sh) MC-deficient mice. All mice were on the C57BL/6 background. Wild type, Tac1−/−, TnfrI−/−, and Kitw-sh/w-sh mice were purchased from Jackson Laboratories. Nk-1r−/− were previously developed at Harvard University. All rodents were housed under standard environmental conditions and maintained on standard commercial rat/mouse chow and tap water ad libitum. All animals were anesthetized by inhaled isoflurane prior to euthanasia. Proper analgesia for euthanasia was evaluated by palpebral reflex, toe pinch reflex, and corneal reflex. At the experimental endpoint, euthanasia was accomplished by removal of the heart. These studies conformed to the principles of the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and the protocols were approved by the Institutional Animal Care and Use Committees.
4
Mouse Strains for Immunological Studies
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C57BL/6J, KitW-sh/W-sh, Cpa3-Cre, and Il6–/–, Basoph8 (Mcpt8tm1(cre)Lky / J) , and iDTR (Gt(ROSA)26Sortm1(HBEGF)Awai/J) mice were obtained from the Jackson Laboratory (Bar Harbor, ME). St2–/–mice were previously described [60 (link)] and backcrossed 8 generations onto the C57BL/6J strain. Il33–/–mice (C57BL/6J background) were provided by Amgen Inc. Mice were maintained in specific pathogen-free conditions at Northwestern University Center for Comparative Medicine. All protocols were approved by the Northwestern University Animal Care and Use Committee. Isoflurane was used for anesthesia and euthanasia, as appropriate. Bone marrow from Cxcl1–/–mice was kindly provided by Dr. Samithamby Jeyaseelan at Louisiana State University (Baton Rouge, LA) (C57BL/6J background) and were previously described [61 (link)].
5
Genetically Modified Mice for Immunology
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WT (6- to 10-week-old male C57BL/6 mice), Cd19Cre (CD19 deficient), Il10−/−, Rag2−/−, Cd40l−/−, and KitW-sh/W-sh mice on the C57BL/6 background were purchased from The Jackson Laboratory (Bar Harbor, ME). Il5ra−/− (C57BL/6 background) mice were obtained from the RIKEN BioResource Center (Tsukuba, Japan). Il5V/V knock-in (C57BL/6 background; IL-5 deficient) mice were provided by S. Takaki (Department of Immune Regulation, Research Institute, National Center for Global Health and Medicine, Chiba, Japan), and the mice were generated as previously described (38 (link)). The mice were housed in a pathogen-free facility at Konkuk University (Seoul, Korea).
6
Genetic Mouse Models for Immunological Research
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All mice were on C57BL/6 background. WT mice (C57BL/6, Strain #002014), OTII mice (Strain #004194), and KitWSH/WSH (C57BL/6, Strain #005051) were purchased from the Jackson Laboratory. Il9r−/− mice (C57BL/6) were a gift from Dr. Jean-Christophe Renauld61 (link). Il9−/− mice (C57BL/6) were provided by Drs. Andrew McKenzie62 (link), Alexander Kirsch, and Sophie Paczesny. Il9fl/fl mice were generated by Jackson Laboratories using Cas9-mediated targeting as characterized in Ulrich & Kharwadkar et al40 (link). Experiments were performed using 6–16-week-old female and male mice with no observed differences between sexes. All mice were maintained in SPF animal facilities (ambient temperature 70–72° F, humidity 50%, light/dark cycle 12/12 hour). All experiments were performed with the approval of the Indiana University Institutional Animal Care and Use Committee.
7
Mouse Models of Japanese Encephalitis Virus
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Control mice on a C57BL/6 background were purchased from InVivos (Singapore). MC-deficient mice (Kitw-sh/w-sh; ‘Sash’) and iDTR mice were originally purchased from Jackson Laboratories and bred in-house. Mcpt5-Cre mice were provided by Axel Roers, Dresden University, Dresden, Germany. Male mice (6-weeks old) were used for all experiments and littermates randomly assigned to experimental groups. Peripheral JEV infection was performed by i.p. injection of 4 × 107 PFU for SA 14-14-2 and 2 × 107 PFU for Nakayama JEV. CNS JEV infection was performed by i.c.v. injection of 1 × 104 PFU of SA 14-14-2 and 100 PFU of Nakayma JEV. All animal experiments were performed according to protocols and ethical guidelines approved by the SingHealth Institution Animal Care and Use Committee (ref. no.: 2015/SHS/1077).
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