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Integraslice 7550 mm vibrating microtome

Manufactured by Campden Instruments
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Sourced in United Kingdom

The Integraslice 7550 MM is a vibrating microtome designed for precise sectioning of tissue samples. It features a high-quality blade that vibrates to produce uniform and smooth tissue sections. The Integraslice 7550 MM is a versatile instrument suitable for a range of applications requiring thin tissue sections.

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Lab products found in correlation

Frosted microscope slides, by Thermo Fisher Scientific (3 mentions) Fluorescence microscope, by Nikon (3 mentions) 125 ul prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions)

6 protocols using integraslice 7550 mm vibrating microtome

1

Immunohistochemistry of Brain Tissue

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Mice were transcardially perfused with room-temperature 0.1 M Phosphate Buffered Saline (PBS), pH 7.3, followed by ice-cold 4% (w/v) paraformaldehyde in PBS. Brains were extracted and post-fixed with 4% paraformaldehyde in PBS at 4°C. 50 μm coronal sections were cut using an Integraslice 7550 MM vibrating microtome (Campden Instruments). Chicken or goat anti-GFP and chicken anti-mCherry antibodies were used at a 1:2000 dilution. Goat anti-choline acetyltransferase antibody was used at 1:200. Secondary donkey anti-goat or anti-chicken antibodies conjugated to either Alexa 488 or 594 were used at 1:1000 dilution. The Brain BLAQ protocol (Kupferschmidt et al., 2017 (link)) was used for immunohistochemistry following FSCV and electrophysiology.
Cover K.K., Gyawali U., Kerkhoff W.G., Patton M.H., Mu C., White M.G., Marquardt A.E., Roberts B.M., Cheer J.F, & Mathur B.N. (2019). Activation of the Rostral Intralaminar Thalamus Drives Reinforcement through Striatal Dopamine Release. Cell reports, 26(6), 1389-1398.e3.
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2

Minimizing Autofluorescence in Brain Slice Imaging

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A specialized protocol to minimize lipid and aldehyde auto-fluorescence was utilized (Kupferschmidt et al. 2015 (link)) for histochemistry and immunohistochemistry of 250 μm brain slices used for whole-cell electrophysiology. This protocol was used to illustrate expression of ACC terminals in claustrum and claustrum neurons targeted for whole-cell recordings. For these slices, primary rabbit anti-mCherry antibody (1:500; Abcam, Cambridge, MA) and secondary donkey anti-rabbit antibody conjugated to Cy3 (1:500; Jackson ImmunoResearch, West Grove, PA) were used to label viral expression of mCherry protein. Streptavidin conjugated to AlexaFluor®-488 (1:1,000; Jackson ImmunoResearch) was used to label neurobiotin-filled neurons.
To obtain representative images of retrograde tract tracer injections, mice injected with retrograde BDA were transcardially perfused with room temperature 0.1M phosphate-buffered saline (PBS), pH 7.2-7.4, followed by ice-cold 4% (weight/volume) paraformaldehyde in PBS. After the brain was extracted, brains were post-fixed with 4% paraformaldehyde in PBS overnight at 4°C. Coronal sections were sliced using an Integraslice 7550 MM vibrating microtome (Campden Instruments, Loughborough, England) at a thickness of 50 μm. Sections were immediately mounted and imaged to visualize retrograde BDA fluorescence.
White M.G, & Mathur B.N. (2018). Claustrum circuit components for top-down input processing and cortical broadcast. Brain structure & function, 223(9), 3945-3958.
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3

Immunohistochemistry of Brain Tissue

Cited 1 time
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Mice were transcardially perfused with room-temperature 0.1 M Phosphate Buffered Saline (PBS), pH 7.3, followed by ice-cold 4% (w/v) paraformaldehyde in PBS. Brains were extracted and post-fixed with 4% paraformaldehyde in PBS at 4°C. 50 μm coronal sections were cut using an Integraslice 7550 MM vibrating microtome (Campden Instruments). Chicken or goat anti-GFP and chicken anti-mCherry antibodies were used at a 1:2000 dilution. Goat anti-choline acetyltransferase antibody was used at 1:200. Secondary donkey anti-goat or anti-chicken antibodies conjugated to either Alexa 488 or 594 were used at 1:1000 dilution. The Brain BLAQ protocol (Kupferschmidt et al., 2017 (link)) was used for immunohistochemistry following FSCV and electrophysiology.
Cover K.K., Gyawali U., Kerkhoff W.G., Patton M.H., Mu C., White M.G., Marquardt A.E., Roberts B.M., Cheer J.F, & Mathur B.N. (2019). Activation of the Rostral Intralaminar Thalamus Drives Reinforcement through Striatal Dopamine Release. Cell reports, 26(6), 1389-1398.e3.
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4

Brain Tissue Preparation and Imaging

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Mice were overdosed on isoflurane gas and perfused with room temperature 0.1M phosphate-buffered solution (PBS), pH 7.3, and then with ice-cold 4% paraformaldehyde (PFA) solution in PBS, 10 days after viral injection surgery. After extraction, the brains were post-fixed in 4% PFA solution overnight. 50 μm thickness slices were obtained using the Integraslice 7550 MM vibrating microtome (Campden Instruments, Loughborough, England), and were stored at 4°C in 0.1M PBS. The slices were mounted onto 25 × 75 × 1 mm frosted microscope slides (Thermo-Scientific, Waltham, MA, United States) using 125 uL ProLong Gold antifade reagent (Invitrogen) as the mountant. The slides were imaged using a Nikon fluorescence microscope (Nikon, Minato, Tokyo, Japan) with images obtained using both 4X and 10X magnification objectives.
Qadir H., Stewart B.W., VanRyzin J.W., Wu Q., Chen S., Seminowicz D.A, & Mathur B.N. (2022). The mouse claustrum synaptically connects cortical network motifs. Cell reports, 41(12), 111860.
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5

Brain Tissue Preparation and Imaging

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Mice were overdosed on isoflurane gas and perfused with room temperature 0.1M phosphate-buffered solution (PBS), pH 7.3, and then with ice-cold 4% paraformaldehyde (PFA) solution in PBS, 10 days after viral injection surgery. After extraction, the brains were post-fixed in 4% PFA solution overnight. 50 μm thickness slices were obtained using the Integraslice 7550 MM vibrating microtome (Campden Instruments, Loughborough, England), and were stored at 4°C in 0.1M PBS. The slices were mounted onto 25 × 75 × 1 mm frosted microscope slides (Thermo-Scientific, Waltham, MA, United States) using 125 uL ProLong Gold antifade reagent (Invitrogen) as the mountant. The slides were imaged using a Nikon fluorescence microscope (Nikon, Minato, Tokyo, Japan) with images obtained using both 4X and 10X magnification objectives.
Qadir H., Stewart B.W., VanRyzin J.W., Wu Q., Chen S., Seminowicz D.A, & Mathur B.N. (2022). The mouse claustrum synaptically connects cortical network motifs. Cell reports, 41(12), 111860.
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6

Viral Tracer Imaging in Mouse Brain

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Mice were overdosed on isoflurane and perfused with room temperature 0.1 M phosphate-buffered solution (PBS), pH 7.3, and then with ice-cold 4% paraformaldehyde (PFA) solution in PBS, 10 days after viral injection surgery. After extraction, the brains were post-fixed in 4% PFA solution overnight. Fifty micrometer thickness slices were obtained using the Integraslice 7550 MM vibrating microtome (Campden Instruments, Loughborough, England) and were stored at 4°C in 0.1 M PBS. The slices were mounted onto 25 × 75 × 1 mm frosted microscope slides (Thermo-Scientific, Waltham, MA, United States) using 127 μL of ProLong Gold antifade reagent (Invitrogen, Carlsbad, CA, United States) as the mountant. The slides were imaged using a Nikon fluorescence microscope (Nikon, Minato, Tokyo, Japan) with images obtained using both 4× and 10× magnification objectives. 10× images of slice sections were obtained and stitched together with 10% blending to create a complete field of view image of the entire brain slice. Adobe Illustrator was used to create chartings depicting viral tracer deposits.
Qadir H., Krimmel S.R., Mu C., Poulopoulos A., Seminowicz D.A, & Mathur B.N. (2018). Structural Connectivity of the Anterior Cingulate Cortex, Claustrum, and the Anterior Insula of the Mouse. Frontiers in Neuroanatomy, 12, 100.
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