Desmoglein 2

Manufactured by Santa Cruz Biotechnology

Desmoglein-2 is a cell adhesion protein that functions in the formation and maintenance of desmosomes, which are specialized cell-cell junctions found in epithelial and certain other cell types. It plays a crucial role in cell-cell adhesion and the structural integrity of tissues.

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Lab products found in correlation

Gallios flow cytometer, by BD (1 mentions) 7 aminoactinomycin d 7 aad, by BD (1 mentions) Kaluza software, by BD (1 mentions) Icosl, by BioLegend (1 mentions) Ox40l, by BD (1 mentions) Protease and phosphatase cocktail, by Thermo Fisher Scientific (1 mentions) Phospho gsk3α, by Cell Signaling Technology (1 mentions) Timp3, by Abcam (1 mentions) Gsk3α, by Cell Signaling Technology (1 mentions) Desmocollin 1, by Santa Cruz Biotechnology (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Pierce bca assay kit, by Thermo Fisher Scientific (1 mentions) Connexin 43, by Merck Group (1 mentions) β catenin, by BD (1 mentions) Non phospho β catenin, by Cell Signaling Technology (1 mentions) C fos, by Santa Cruz Biotechnology (1 mentions) Connexin 26, by Santa Cruz Biotechnology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Desmoglein (2 citations) Desmoglein 2 (DSG2) (clone AH12.2, (2 citations)

2 protocols using desmoglein 2

Monoclonal Antibody Staining for Flow Cytometry

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The following monoclonal antibodies conjugated with fluorochrome were used: Desmoglein-2(Santa Cruz Biotechnology), 41BBL, ICOSL (BioLegend), OX40L, CAR, CD46 (BD Biosciences), FAP (R&D Systems). Cells were stained with these antibodies for 30 min at 4 °C. Live/dead discrimination was determined via inclusion of 7-aminoactinomycin D (7AAD; BD Pharmingen). Stained cells were analyzed using Gallios flow cytometer with Kaluza software (BD Biosciences) according to the manufacturer’s instructions.

Rosewell Shaw A., Porter C., Biegert G., Jatta L, & Suzuki M. (2022). HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy. Cancers, 14(11), 2769.

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Protein Expression Analysis of Cell Lysates

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After desired treatments, cells were lysed with RIPA buffer spiked with a fresh protease and phosphatase cocktail (Thermo Scientific, #78442) and sonicated. Protein concentrations were quantified using the Pierce BCA assay kit (Thermo Fisher, #23225). 80–120μg of protein for each sample was loaded onto SDS-PAGE gels, and then transferred onto PVDF membranes. The blots were incubated with the following antibodies: desmocollin 1 (sc-398590, RRID: AB_2894905), desmoglein 2 (sc-80663, RRID: AB_2093438), plakophilin (sc-33636, RRID: AB_2164139), connexin 26 (sc-7261, RRID: AB_2110895) and cFOS (sc-52, RRID: AB_2106783) from Santa Cruz; ER-α (#8644, RRID: AB_2617128), HA (#3724, RRID: AB_1549585), Non-phospho-β-catenin (#19807, RRID: AB_2650576), Histone H3 (#4499, RRID: AB_10544537), AIF (#5318, RRID: AB_10634755), GSK3β (Ser9, #5558, RRID: AB_10013750), phospho-GSK3α (Ser21, #9316, RRID: AB_659836), GSK3β (#12456, RRID: AB_2636978) and GSK3α (#4337, RRID: AB_10859910) from Cell Signaling Technology; β-catenin (#610154, RRID: AB_397555) from BD; Tubulin (T6557, RRID: AB_477584) and connexin 43 (C6219, RRID: AB_476857) from Sigma Aldrich; and TIMP3 (ab39184, RRID: AB_2204971) from Abcam.

Li Z., Wu Y., Yates M.E., Tasdemir N., Bahreini A., Chen J., Levine K.M., Priedigkeit N.M., Nasrazadani A., Ali S., Buluwela L., Arnesen S., Gertz J., Richer J.K., Troness B., El-Ashry D., Zhang Q., Gerratana L., Zhang Y., Cristofanilli M., Montanez M.A., Sundd P., Wallace C.T., Watkins S.C., Fumagalli C., Guerini-Rocco E., Zhu L., Tseng G.C., Wagle N., Carroll J.S., Jank P., Denkert C., Karsten M.M., Blohmer J.U., Park B.H., Lucas P.C., Atkinson J.M., Lee A.V, & Oesterreich S. (2022). Hotspot ESR1 mutations are multimodal and contextual modulators of breast cancer metastasis. Cancer research, 82(7), 1321-1339.

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