Rabbit antibody to gfp conjugated with alexa488

Manufactured by Thermo Fisher Scientific

The Rabbit antibody to GFP conjugated with Alexa488 is a fluorescently labeled antibody that binds to the Green Fluorescent Protein (GFP). This product is designed for use in various immunodetection and immunolocalization applications.

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Lab products found in correlation

Mouse antibody to myc 4e10, by Santa Cruz Biotechnology (2 mentions) Rabbit antibody to rfp, by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

GFP antibody (43 citations) Alexa488-conjugated antibody (6 citations) Rabbit GFP antibody (3 citations) Rabbit antibody to GFP (2 citations) Alexa488 antibody (2 citations)

2 protocols using rabbit antibody to gfp conjugated with alexa488

Drosophila Brain Immunostaining and Imaging

Cited 1 time

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The experimental procedures for brain dissection, fixation, and immunostaining as well as agarose section were as described previously (Hakeda-Suzuki et al., 2011 (link)). The following primary antibodies were used: mAb24B10 (1:50, DSHB), rat antibody to CadN (Ex#8, 1:50, DSHB), mouse antibody to Repo (8D12, 1:20, DSHB) mouse antibody to myc (4E10, 1:100, Santa Cruz), rabbit antibody to RFP (1:500 ROCKLAND), rabbit antibody to GFP conjugated with Alexa488 (1:200, Life technologies). The secondary antibodies were Alexa488, Alexa568, or Alexa633-conjugated (1:400, Life technologies). Images were obtained with Nikon C2⁺ and A1 confocal microscopes and processed with Adobe Photoshop and Illustrator.
Live imaging was done according to Özel et al., 2015 (link). Images were obtained with Zeiss LSM880NLO + COHERENT Chameleon Vision.

Takechi H., Hakeda-Suzuki S., Nitta Y., Ishiwata Y., Iwanaga R., Sato M., Sugie A, & Suzuki T. (2021). Glial insulin regulates cooperative or antagonistic Golden goal/Flamingo interactions during photoreceptor axon guidance. eLife, 10, e66718.

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Brain Dissection and Immunostaining Protocol

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The experimental procedures for brain dissection, fixation and immunostaining as well as agarose section were as described previously (Hakeda-Suzuki et al., 2011) . The following primary antibodies were used: mAb24B10 (1:50, DSHB), rat antibody to CadN (Ex#8, 1:50, DSHB), mouse antibody to Repo (8D12, 1:20, DSHB) mouse antibody to myc (4E10, 1:100, Santa Cruz), rabbit antibody to RFP (1:500 ROCKLAND), rabbit antibody to GFP conjugated with Alexa488 (1:200, Life technologies). The secondary antibodies were Alexa488, Alexa568 or Alexa633-conjugated (1:400, Life technologies). Images were obtained with Nikon C2 + and A1 confocal microscopes and processed with Adobe Photoshop and Illustrator.
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted January 24, 2020. ; https://doi.org/10.1101/2020.01.23.916403 doi: bioRxiv preprint 7
Live imaging was done according to (Özel et al., 2015) . Images were obtained with Zeiss LSM880NLO + COHERENT Chameleon Vision.

Takechi H., Hakeda‐Suzuki S., Nitta Y., Ishiwata Y., Sato M., Sugie A., & Suzuki T. (2020). Glial insulin regulates cooperative or antagonistic Golden goal/Flamingo interactions during photoreceptor axon navigation.

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