Planar gamma imager

For planar imaging of organs, animals were anesthetized with Isoflurane (Baxter) and ¹¹¹In labeled nanoparticles were intravenously injected into the tail vein. Animals were sacrificed 15 min or 1 h post injection, organs were harvested and placed on a planar gamma-imager (Biospace) equipped with a high energy collimator. Images were recorded at the indicated time points with 10 min acquisition time.

All mice experiments were carried out in accordance with German legislation on animal welfare. Female NMRI mice (6–8 weeks) were obtained from Janvier Laboratories. For planar imaging, mice were anesthetized with Isoflurane (Baxter) and ¹¹¹In labeled nanoparticles with a total activity of 8–10 MBq (corresponding to 100 µL) were intravenously injected into the tail vein. Afterwards, the animals were placed in prone position (see Figure 5—figure supplements 2A and 3A) on a planar gamma-imager (Biospace) equipped with a high energy collimator as described previously (Müller et al., 2013 (link); Wischnjow et al., 2016 (link)). Images were recorded at the indicated time points with 10 min acquisition time.