Acetonitrile lc gradient grade

Acetonitrile (LC gradient grade) were purchased from VWR International (Leuven, Belgium), methanol (LC gradient grade) and glacial acetic acid (p.a.) from Merck (Darmstadt, Germany), and ammonium acetate MS grade) from Sigma-Aldrich (Vienna, Austria). Water was purified successively by reverse osmosis and an Elga Purelab ultra analytic system from Veolia Water (Bucks, UK) to 18.2 MΩ. Standards of fungal and bacterial metabolites were obtained either as gifts from various research groups or from the following commercial sources: Romer Labs^® Inc. (Tulln, Austria), Sigma-Aldrich (Vienna, Austria), Iris Biotech GmbH (Marktredwitz, Germany), Axxora Europe (Lausanne, Switzerland) and LGC Promochem GmbH (Wesel, Germany), Enzo Life Sciences (Lausen, Switzerland), BioAustralis (Smithfield, Australia), AnalytiCon Discovery (Potsdam, Germany) and Toronto Research Chemicals (Toronto, ON, Canada).

Methanol (LC gradient grade) and glacial acetic acid (p.a) were purchased from Merck (Darmstadt, Germany), acetonitrile (LC gradient grade) from VWR (Leuven, Belgium), and ammonium acetate (MS grade) from Sigma-Aldrich (Vienna, Austria). Mycotoxin standards were donated by various research groups or purchased from various commercial sources. Water was purified successively by reverse osmosis using an Elga Purelab ultra-analytic system from Veolia Water (Bucks, UK).

Methanol (LC gradient grade) and formic acid (p.a.) were purchased from Merck (Darmstadt, Germany), acetonitrile (LC gradient grade) from VWR (Leuven, Belgium), and ammonium acetate (MS grade) from Sigma-Aldrich (Schnelldorf, Germany). Water was purified using an Elga Purelab ultra analytic system (Veolia Water, Buckinghamshire, UK). Deoxynivalenol-3-sulfate (D3S) and deoxynivalenol-15-sulfate (D15S) (Fig. 1) were synthesized using a sulfuryl imidazolium salt as described by Fruhmann et al. [16 (link)]. D3G and DON for analytical purpose were purchased from Romer Labs Diagnostic GmbH (Tulln, Austria), whereas DON for the treatment of plants was produced and purified according to Altpeter and Posselt [17 (link)]. Solid substances were dissolved in pure methanol (D3S, D15S) or acetonitrile (DON, D3G) and stored at −20 °C. A combined multi-standard working solution for preparation of calibrants and spiking experiments was prepared in acetonitrile containing 5.0 mg/L of each analyte.Fig. 1

Structure of deoxynivalenol (DON) and its plant metabolites DON-3-sulfate and DON-15-sulfate