1kms600cw ccd camera

Manufactured by Ametek

The 1kMS600CW CCD camera is a high-resolution imaging device designed for scientific and industrial applications. It features a CCD (Charge-Coupled Device) sensor with a resolution of 1024x600 pixels. The camera is capable of capturing images with a high signal-to-noise ratio and is suitable for a variety of laboratory and research tasks.

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Lab products found in correlation

Copper palladium tem grids, by Agar Scientific (8 mentions) Tecnai spirit tem instrument, by Ametek (5 mentions) Tecnai spirit microscope, by Ametek (2 mentions)

8 protocols using 1kms600cw ccd camera

Transmission Electron Microscopy of Nanoparticle Dispersions

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Solutions were
diluted 100-fold at either 20 or 5 °C to generate 0.10% w/w dispersions.
Images obtained at lower pH were prepared by diluting dispersions
using acidified water at the desired solution pH. Copper/palladium
TEM grids (Agar Scientific, UK) were surface-coated in-house to yield
a thin film of amorphous carbon. The grids were then plasma glow-discharged
for 30 s to create a hydrophilic surface. Individual samples (0.10%
w/w, 12 μL) were adsorbed onto the freshly glow-discharged grids
for 60 s and then blotted with filter paper to remove excess solution.
To stain the aggregates, a 9 μL drop of 0.75% w/w uranyl formate
solution was soaked on the sample-loaded grid for 20 s and then carefully
blotted to remove excess stain. The grids were then dried using a
vacuum hose. Imaging was performed at 80 kV using a FEI Tecnai Spirit
microscope equipped with a Gatan 1kMS600CW CCD camera.

Lovett J.R., Warren N.J., Armes S.P., Smallridge M.J, & Cracknell R.B. (2016). Order–Order Morphological Transitions for Dual Stimulus Responsive Diblock Copolymer Vesicles. Macromolecules, 49(3), 1016-1025.

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Transmission Electron Microscopy of Copolymer Nanoparticles

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0.20% w/w copolymer dispersions were prepared at 20 °C. Copper/palladium TEM grids (Agar Scientific, UK) were surface-coated in-house to produce a thin film of amorphous carbon, then plasma glow-discharged for 30 seconds to create a hydrophilic surface. Droplets of freshly-prepared aqueous copolymer dispersions (9 μL; 0.20% w/w) were placed on a hydrophilic grid for 1 min and then blotted with filter paper to remove excess solution. The deposited nanoparticles were then negatively stained with an aqueous solution of uranyl formate (9 μL; 0.75% w/w) for a further 20 seconds, then carefully blotted to remove excess stain and dried with a vacuum hose. TEM grids were imaged using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW CCD camera operating at 120 kV.

Ratcliffe L.P., Bentley K.J., Wehr R., Warren N.J., Saunders B.R, & Armes S.P. (2017). Cationic disulfide-functionalized worm gels †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7py01306j. Polymer Chemistry, 8(38), 5962-5971.

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Copolymer Nanoparticle Characterization by TEM

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Copolymer dispersions
were diluted 50-fold at 20 °C to generate 0.20% w/w dispersions.
Copper/palladium TEM grids (Agar Scientific, UK) were coated in-house
to produce a thin film of amorphous carbon. These grids were then
treated with a plasma glow discharge for 30 s to create a hydrophilic
surface. One droplet of each aqueous diblock copolymer dispersion
(12 μL; 0.20% w/w) was placed on a freshly treated grid for
1 min and then blotted with filter paper to remove excess solution.
To stain the deposited nanoparticles, an aqueous solution of uranyl
formate (9 μL; 0.75% w/w) was placed on the sample-loaded grid
via micropipet for 20 s and then carefully blotted to remove excess
stain. Each grid was then carefully dried using a vacuum hose. Imaging
was performed using a FEI Tecnai Spirit TEM instrument equipped with
a Gatan 1kMS600CW CCD camera operating at 120 kV.

Jesson C.P., Cunningham V.J., Smallridge M.J, & Armes S.P. (2018). Synthesis of High Molecular Weight Poly(glycerol monomethacrylate) via RAFT Emulsion Polymerization of Isopropylideneglycerol Methacrylate. Macromolecules, 51(9), 3221-3232.

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Copper/Palladium Nanoparticle TEM Imaging

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Copper/palladium
TEM grids (Agar Scientific, U.K.) were surface-coated with a thin
film of amorphous carbon. If required, grids were subjected to a plasma
glow discharge for 30 s to produce a hydrophilic surface. One droplet
of an aqueous dispersion of nanoparticles (0.2% w/w, 10 μL)
or a nanoemulsion (0.5% v/v, 10 μL) was placed on a grid for
1 min, after which any remaining solution was removed by blotting
with filter paper. Subsequently, an aqueous droplet of uranyl formate
(0.75% w/w, 10 μL) was placed on the sample-loaded grid for
20 s, and the excess stain was removed by blotting. Each grid was
carefully dried using a vacuum hose. Images were recorded using an
FEI Tecnai Spirit microscope operating at 80 kV and equipped with
a Gatan 1kMS600CW CCD camera.

Hunter S.J., Chohan P., Varlas S, & Armes S.P. (2024). Effect of Temperature, Oil Type, and Copolymer Concentration on the Long-Term Stability of Oil-in-Water Pickering Nanoemulsions Prepared Using Diblock Copolymer Nanoparticles. Langmuir, 40(7), 3702-3714.

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Transmission Electron Microscopy of Copolymer Dispersions

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TEM imaging
was performed on 0.1% w/w copolymer dispersions. Copper/palladium
TEM grids (Agar Scientific, U.K.) surface-coated in-house by a thin
film of amorphous carbon were used as sample substrates. Before a
sample deposition, the substrate surface was treated by plasma glow
discharge for 30 s to make it hydrophilic. A copolymer solution (0.1%
w/w, 9 μL) was pipetted onto a freshly glow discharged grid
for 20 s and then blotted to remove the excess using filter paper.
These were negatively stained using an aqueous solution of uranyl
formate (0.75% w/w, 9 μL). The sample-loaded grid was exposed
to the stain for 30 s before removing the excess by blotting. Grids
were dried using a vacuum hose. Imaging was performed at 120 kV using
a FEI Tecnai G2 Spirit TEM instrument equipped with a Gatan 1kMS600CW
CCD camera.

Neal T.J., Parnell A.J., King S.M., Beattie D.L., Murray M.W., Williams N.S., Emmett S.N., Armes S.P., Spain S.G, & Mykhaylyk O.O. (2021). Control of Particle Size in the Self-Assembly of Amphiphilic Statistical Copolymers. Macromolecules, 54(3), 1425-1440.

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Transmission Electron Microscopy of Diblock Copolymer Nanoparticles

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Diblock copolymer
dispersions were diluted 50-fold to 0.20% w/w at 20 °C (for G₅₄-H₁₄₀ and G₇₁-H₂₀₀) or 50
°C (for G₃₇-H₈₀) prior to staining. Copper/palladium
TEM grids (Agar Scientific, UK) were surface-coated in-house to yield
a thin film of amorphous carbon. The grids were then treated with
a plasma glow discharge for 45 s to create a hydrophilic surface.
One 12 μL droplet of each aqueous copolymer dispersion was then
placed onto a freshly treated grid for 30 s and then blotted with
filter paper to remove excess solution. To stain the deposited nanoparticles,
a 0.75% w/w aqueous solution of uranyl formate (9 μL) was placed
via micropipet on the sample-loaded grid for 20 s and then carefully
blotted to remove excess stain. Each grid was then carefully dried
using a vacuum hose. Imaging was performed using a FEI Tecnai Spirit
TEM instrument equipped with a Gatan 1kMS600CW CCD camera and operating
at 80 kV.

Warren N.J., Derry M.J., Mykhaylyk O.O., Lovett J.R., Ratcliffe L.P., Ladmiral V., Blanazs A., Fielding L.A, & Armes S.P. (2018). Critical Dependence of Molecular Weight on Thermoresponsive Behavior of Diblock Copolymer Worm Gels in Aqueous Solution. Macromolecules, 51(21), 8357-8371.

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Copolymer Nanoparticle Visualization via TEM

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Copolymer dispersions
were diluted fifty-fold at 20 °C to generate 0.20% w/w dispersions.
Copper/palladium TEM grids (Agar Scientific, UK) were coated in-house
to produce a thin film of amorphous carbon. These grids were then
treated with a plasma glow discharge for 30 s to create a hydrophilic
surface. Each aqueous diblock copolymer dispersion (12 μL; 0.20%
w/w) was placed on a freshly treated grid for 1 min and then blotted
with filter paper to remove excess solution. To stain the deposited
nanoparticles, an aqueous solution of uranyl formate (9 μL;
0.75% w/w) was placed on the sample-loaded grid via a micropipet for
20 s and then carefully blotted to remove excess stain. Each grid
was then carefully dried using a vacuum hose. Imaging was performed
using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW
CCD camera operating at 120 kV.

Jesson C.P., Pearce C.M., Simon H., Werner A., Cunningham V.J., Lovett J.R., Smallridge M.J., Warren N.J, & Armes S.P. (2016). H2O2 Enables Convenient Removal of RAFT End-Groups from Block Copolymer Nano-Objects Prepared via Polymerization-Induced Self-Assembly in Water. Macromolecules, 50(1), 182-191.

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Transmission Electron Microscopy of Polymer Vesicles

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Aqueous copolymer dispersions were diluted at 20 °C to generate 0.10% w/w dispersions. Copper/palladium TEM grids (Agar Scientific, UK) were surface-coated in-house to yield a thin film of amorphous carbon. The grids were then plasma glow-discharged for 30 s to create a hydrophilic surface. One 12 μL droplet of each dispersion was adsorbed onto a freshly glow-discharged grid for 20 s and then blotted with filter paper to remove excess solution. To improve the contrast of the PGMA–PHPMA vesicles, uranyl formate (9 μL of a 0.75% w/v aqueous solution) was placed on each sample-loaded grid for 20 s and then carefully blotted to remove excess stain. To stain the PGMA–PHPMA–PDPA triblock copolymer aggregates, phosphotungstic acid (9 μL of a 1.0% w/v solution) was placed on each sample-loaded grid for 5 s and then carefully blotted to remove excess stain. Each grid was then dried using a vacuum hose. Imaging was performed using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW CCD camera operating at 120 kV.

Mable C.J., Fielding L.A., Derry M.J., Mykhaylyk O.O., Chambon P, & Armes S.P. (2017). Synthesis and pH-responsive dissociation of framboidal ABC triblock copolymer vesicles in aqueous solution †Electronic supplementary information (ESI) available: Full experimental details for the synthesis and characterisation of the diblock and triblock copolymer vesicles, assigned 1H NMR spectra, GPC traces, digital images of dispersions, additional plots of pH vs. hydrodynamic diameter, count rate and zeta potential, a table of SAXS parameters, extra data for G58H250D184 and un-merged SAXS patterns are provided. See DOI: 10.1039/c7sc04788f. Chemical Science, 9(6), 1454-1463.

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