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3 protocols using magnesium nitrate mg no3 2

1

Synthesis of Metal-Silica Nanomaterials

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All chemicals used in the present study were of analytical grade and used without further purification. Metal salts: europium nitrate Eu(NO3)3 was prepared by reactions of europium oxide (Belami Fine Chemical) and nitric acid. Strontium chloride SrCl2, copper chloride CuCl2·2H2O, lead nitrate Pb(NO3)2, nickel nitrate hexahydrate Ni(NO3)2·6H2O, ferric chloride hexahydrate FeCl3·6H2O, magnesium nitrate Mg(NO3)2, manganese chloride MnCl2, mercuric nitrate Hg(NO3)2, tin(iv) chloride pentahydrate SnCl4·5H2O and cobalt chloride CoCl2 were obtained from Sigma. 3-Aminopropyl trimethoxysilane (APTS) as a silylating agent, purchased from Fluka. Acetic acid, NaOH and hydrochloric acid were obtained from Sigma-Aldrich.
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2

EVOH 32 Polymer Synthesis Protocol

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Poly(vinyl alcohol-co-ethylene) with 32 mol% ethylene content (EVOH 32) (density: 1.19 g/mL at 25 °C, melt index: 3.8 g/10 min (210 °C)), 1-Propanol reagent, ≥99.5%, magnesium nitrate (Mg(NO3)2), zinc acetate, and hexamethylenetetramine were provided from Sigma-Aldrich® (Milan, Italy).
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3

Enzymatic Pyruvate Oxidation with GQD

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Pyruvate oxidase (PoxB,
EC: 1.2.3.3) from microorganisms, sodium pyruvate (Py), glutaraldehyde
(GA, 25% in H2O), Nafion (5 wt %), GQDs (cat. no. 900713,
less than 5 nm in diameter), sodium phosphate monobasic (NaH2PO4), sodium phosphate dibasic (Na2HPO4), potassium chloride (KCl), potassium ferricyanide (K3[Fe(CN)6]), iron (III) chloride (FeCl3), magnesium nitrate (Mg(NO3)2), deuterated
water (D2O), and N,N-dimethylglycine
(DMG, C4H9NO2) were purchased from
Sigma-Aldrich (USA). TPP was purchased from Koram Biotech Corporation
(South Korea). All other reagents were of analytical grade and used
without further purification. The pH optimization experiment used
phosphate-buffered solutions (PBS, 50 mM) at different pH values,
which were prepared by mixing NaH2PO4 with Na2HPO4 and 100 mM KCl. All aqueous solutions were
prepared using deionized water (Milli-Q water purifying system, 18
MΩ cm).
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