Fenpyroximate

The chemical library was donated by the EPA ToxCast Program. For verification and replication experiments, the following chemicals were purchased from Sigma-Aldrich: DL-α tocopherol acetate (T3376), DL-sulforophane (S4441), vincristine sulfate salt (V8879), oxyfluorfen (35031), rotenone (45656), fenamidone (33965), pyraclostrobin (33696), trifloxystrobin (46447), myxothiazol (T5580), pyridaben (46047), azoxystrobin (31697), fluoxastrobin (33797), fenpyroximate (31684) and kresoxim-methyl (37899). Famoxadone was purchased from Chem Service, Inc (N-11943). Topotecan hydrochloride was purchased from Tocris (4562). Paclitaxel was purchased from Fisher Scientific (AC32842). All chemical stocks were prepared in DMSO unless otherwise noted. Vehicle samples were prepared with an equivalent DMSO concentration of ≤0.5% in feeding medium.

Clofentezine, fenpyroximate and pyridaben with a certified purity >98% were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Stock standard solutions of Clofentezine, fenpyroximate and pyridaben were prepared in Methanol (5.0 mL), with concentration of 1000 mg L⁻¹ and was stored in a freezer at −20 °C. The working standard solutions were prepared daily by diluting the stock solutions with water to the required concentrations. The ultra-pure water was purchased from Shahid Ghazi Pharmaceutical Co. (Tabriz, Iran). Methanol, acetonitrile, phosphate salt (analytical grade), ethylene glycol, n-butanol, glycerol, n-heptanol, n-nonanol and NaCl were obtained from Merck (Darmstadt, Germany). MTOAC with purity higher than 97% were purchased by Aladdin Biochemical Co., Ltd (Shanghai, China).