Achilles tendon 2/3 transection was conducted at the midpoint level between the calcaneal insertion and the musculotendinous junction of the right Achilles tendon. All transected tendons were repaired with a 2-strand modified Kessler technique with surface locking followed by a simple peripheral suture (8–0 Ethilon, Ethicon Inc; Fig. 2A ).34 Following repair, mice were allowed free movement after recovery from anesthesia.
8 0 ethilon
Manufactured by Johnson & Johnson
Sourced in United States
The 8–0 Ethilon is a sterile, monofilament, non-absorbable surgical suture made of nylon. It is designed for use in delicate ophthalmic and microsurgical procedures. The suture has a very fine diameter and is intended for precise wound closure.
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Lab products found in correlation
5 protocols using 8 0 ethilon
1
Achilles Tendon Transection and Repair
2
Ileal Anastomosis Protocol
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Anastomoses were created as described previously17 . Briefly, 1 cm of ileum was resected at 15 cm proximal to the caecum. An inverted anastomosis was made with a single layer of eight interrupted (Lembert) sutures (8/0 Ethilon®; Ethicon, Norderstedt, Germany). After operation, rats were housed individually because they were attached to the swivel system.
3
Murine Pericardial Infarct Model for Cardiac Biomaterial Evaluation
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In vivo studies used a murine intact pericardium infarct model. Surgical infarction was performed as described previously.25 (link),35 (link) Anesthetic and ventilation strategies were as described for noninfarcted mice. Left-sided thoracotomy was performed through the third interspace. The anterior interventricular artery was then visualized through the translucent pericardium. No pericardiotomy was performed because an intact pericardium was needed for the biomaterial injection. Instead, a suture (8-0 Ethilon [Ethicon]) was placed through the pericardium, surrounding the proximal anterior interventricular artery, and back through the pericardium; infarction was induced by tying down the vessel. After infarction, mice were either injected with a 40-μL suspension of mSIS-ECM in normal saline (treatment) or 40 μL of normal saline (control). Layered chest closure was then performed with 5-0 Vicryl (Ethicon), with gentle suction provided using a 24-G catheter to evacuate residual thoracic air. Animals were sacrificed for cardiac explantation at 7 days for flow cytometry and assessment of myocardial homogenates and at 28 days for resonant confocal microscopy and cardiac functional analysis.
4
Partial Sciatic Nerve Ligation Model of Neuropathic Pain
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The nociceptive study was carried out in the partial sciatic nerve ligation model of traumatic neuropathy, which is a well-established, highly reproducible, and easily performable model of neuropathic pain characterized by mechanical hyperalgesia [25 (link),26 (link)]. It is widely used to examine the effects of novel analgesic candidates for the unmet need of these medical conditions [27 (link),28 (link),29 (link),30 (link)].
Partial sciatic nerve ligation was performed in male NMRI mice under ketamine/xylazine (100/5 mg/kg, i.p.) anesthesia, as previously described [25 (link)]. Briefly, the ipsilateral common sciatic nerve was exposed high in the thigh region and one-third to one-half of the nerve thickness was tightly ligated using a siliconized silk suture (Ethilon 8-0, Ethicon, Somerville, NJ, USA). The wound was closed with a skin suture (Mersilk 4-0, Ethicon, Somerville, NJ, USA), and the animals were allowed to survive for 7 days, because significant mechanical hyperalgesia develops without postoperative pain component by this time point [20 (link)].
Partial sciatic nerve ligation was performed in male NMRI mice under ketamine/xylazine (100/5 mg/kg, i.p.) anesthesia, as previously described [25 (link)]. Briefly, the ipsilateral common sciatic nerve was exposed high in the thigh region and one-third to one-half of the nerve thickness was tightly ligated using a siliconized silk suture (Ethilon 8-0, Ethicon, Somerville, NJ, USA). The wound was closed with a skin suture (Mersilk 4-0, Ethicon, Somerville, NJ, USA), and the animals were allowed to survive for 7 days, because significant mechanical hyperalgesia develops without postoperative pain component by this time point [20 (link)].
5
Endothelial Role in Mirabegron Vascular Effects
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To investigate the possible role of endothelium-derived relaxing substances different from NO in the vascular response to mirabegron, vessels (n = 4) were denuded of endothelium by surface abrasion, gently inserting and removing a nylon suture (Ethilon 8-0; Ethicon, Somerville, NJ, USA) for 50× in the lumen, before mounting them in the myograph chamber; the success of the endothelial removal procedure was confirmed by absent relaxation of vessels to 1 μmol L -1 BK following precontraction with U46619 (1 μmol L -1 ). Afterwards, arteries were contracted with PE (10 μmol L -1 ) and the CCRC to mirabegron (10 -7 to 10 -4 mol L -1 ) was constructed in each segment. Data are reported as means ± SEM.
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