Cobas c702 chemistry analyzer

Blood was drawn from the patient’s brachial veins and placed in a vial containing clot activator and a vial containing the anticoagulant tripotassium ethylenediaminetetraacetic acid (K3 EDTA). Immediately after blood collection, the vials were taken to the department of medical laboratory diagnostics. Serum from the vial with clot activator was used to measure CRP levels. Blood collected in a vial containing K3 EDTA was used to analyze white blood cell count and total neutrophils. An analysis of leukocyte and total neutrophil counts was performed in a routine laboratory using a hematological blood analyzer (Siemens Advia 2120 Hematology Analyzer, Bayer, Germany). A CRP assay was performed by the immunoturbidimetric method using the Cobas C702 chemistry analyzer (Roche, Rotkreuz, Switzerland).

The study participants were recruited to the study by a pediatric endocrinologist, and they and their parents signed a written consent. The Ethics Committee of the Hospital District of Southwest Finland approved the study (108/180/2010).
The clinical examinations were performed by a pediatrician (SK) and thyroid ultrasound, head MRI and bone age by the pediatric radiologists. Laboratory tests were done at the Turku University Hospital Laboratory, except for the IGF-1 test, which was performed in the Islab-laboratory (Kuopio, Finland). Umbilical serum TSH (uS-TSH), serum TSH, free T4 (fT4), insulin and cortisol concentrations were determined with the Cobas e801 immunoassay analyzer (Roche Diagnostics, Rotkreuz, Switzerland). Serum cholesterol (HDL cholesterol, LDL cholesterol and triglycerides) were determined with the Cobas c702 chemistry analyzer (Roche Diagnostics). Serum glycosylated hemoglobin (HbA1c) was determined with the Cobas c501 immunoturbidimetric assay (Roche Diagnostics). Serum IGF-1 concentrations were determined with the Liaison XL chemiluminescence analyzer (DiaSorin S.p.A, Saluggia, Italy). Growth data were collected from the hospital records based on measurements performed at the visits using stabilized and calibrated scale and wall mounted Harpenden Stadiometer (Holtain Limited, Crosswell, Crymych, Pembs., UK) with ±0.1 cm precision.

The liver function parameters (AST, ALT), glucose, uric acid, sodium, and potassium in plasma samples were analyzed using the Roche Cobas C702 Chemistry Analyzer (Roche Diagnostics, Istanbul, Türkiye). AST, ALT, glucose and uric acid were measured using the kinetic enzymatic colorimetric method. Sodium and potassium were determined using the indirect ISE (ion selective electrode) method. The samples were studied in duplicates. Results were given as mean ± standard deviation (range). The units were as follows: U/L for AST and ALT; mg/dL for glucose and uric acid; mEq/L for sodium and potassium.