Multiscreen cellulose filter plates (Millipore) were coated overnight with 10 μg of histone from calf thymus (Sigma-Aldrich). Cells from the mLN were collected and plated starting at 3 × 106/well and in 2-fold serial dilutions in complete RPMI 1640 containing 10% FBS. After 5 h, the wells were washed with PBS containing 0.5% BSA and 0.05% Tween 20, and IgG was detected using alkaline phosphatase-conjugated goat anti-mouse IgG (Jackson immunoresearch). Spots were counted using a dissecting microscope. Images were taken using a CTL-immunoSpot S5 analyzer.
Multiscreen cellulose filter plates
Manufactured by Merck Group
The Multiscreen cellulose filter plates are a type of laboratory equipment used for sample filtration and separation. They feature a cellulose-based filter membrane that enables the efficient separation of particles, cells, or other analytes from liquid samples. The plates are designed to facilitate high-throughput processing and can be used in a variety of applications within the life sciences and research industries.
Automatically generated - may contain errors
2 protocols using multiscreen cellulose filter plates
1
Histone-Specific Antibody Secretion Assay
2
Histone-Specific Antibody Secretion Assay
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