Wild type c57bl 6j female mice

Wild type C57Bl/6J female mice age 6–8 weeks were obtained from Jackson Laboratory, Bar Harbor, ME. MNV-free status of mice from Jackson Laboratory was confirmed by serology testing and qPCR. After arrival, mice were maintained MNV-free in designated cages with bedding and food changes done separately from other mice by the investigators. Periodic monitoring of naïve controls was carried out to confirm MNV-free status. Mice were infected by oral gavage with 1×10⁶ PFU of MNV delivered in 200 μl culture medium (RPMI with 10% fetal bovine serum). For vaccinia virus boost experiments, mice were injected intraperitoneally with 1×10⁶ PFU of rVV^519Y in 200 μl culture medium. All mice were used in accordance with Institutional Animal Care and Use Committee guidelines for the University of Pennsylvania.

All mouse experiments were approved by the Institutional Animal Care and Use Committee, Indiana University School of Medicine and followed the guidelines of the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Visual Research. Wild‐type C57BL/6J female mice, 6–8 weeks of age, plus timed E18-E19 stage pregnant mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). The mice were housed under standard conditions (35 (link)) in the Indiana University Laboratory Animal Resource Center (LARC). Fech^m1Pas mutant mice (36 (link)) were purchased from Jackson Laboratory on a BALB/c background and backcrossed into C57BL/6J; mixed‐sex littermates from an F1 generation were used for experiments at 6–8 weeks of age. These animals were genotyped by qPCR according to a protocol from Jackson Laboratory (https://www2.jax.org/protocolsdb/f?p=116:5:0::NO:5:P5_MASTER_PROTOCOL_ID,P5_JRS_CODE:21675,002662). Sample sizes for experiments were based on power analyses, and treatments were randomly assigned by cage and animals. The experimenter was masked to genotype or treatment during analyses.

All animal procedures were performed according to protocols approved by the Institutional Animal Care and Use Committee at Baylor College of Medicine (AN 6979) and reported following ARRIVE guidelines. Wild-type C57BL6/J female mice were purchased from The Jackson Laboratory (Bar Harbor, Maine, USA) and allowed to acclimate for at least one week before start of the experiments. Animals were housed 2–5 per cage, kept on a 12h light-dark cycle in a temperature-controlled environment (22°C), and had water and standard chow ad libitum.

Wild-type C57 BL/6J female mice (8–12 weeks) were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). All mice were bred in Thomas Jefferson Animal Care facilities, and all experimental procedures were approved by the Institutional Animal Care and Use Committee of Thomas Jefferson University [4 (link), 21 (link)].