Gemini 5u c18 110a column

Manufactured by Phenomenex

Sourced in United States

The Gemini 5u C18 110A column is a high-performance liquid chromatography (HPLC) column designed for a wide range of analytical applications. The column features a 5 micron particle size and a 110 angstrom pore size, providing efficient separation and high-resolution analysis of various analytes. The column is packed with a C18 stationary phase, which is suitable for the separation of a variety of polar and non-polar compounds.

Automatically generated - may contain errors

Lab products found in correlation

600 controller, by Waters Corporation (1 mentions) High performance liquid chromatography system, by Waters Corporation (1 mentions) 2489 uv detector, by Waters Corporation (1 mentions) Lc20 ad hplc system, by Phenomenex (1 mentions) Lc 20a hplc system, by Shimadzu (1 mentions)

Spelling variants of this product

C18 column (366 citations) Gemini C18 (326 citations) Gemini C18 column (288 citations) Gemini column (17 citations) Gemini 5U C18 column (6 citations) Gemini C18 110A column (5 citations) Gemini 5u C18 110A (4 citations) Gemini 5u C18 (3 citations) Gemini-NX 5u C18 110A column (3 citations)

4 protocols using gemini 5u c18 110a column

HPLC Analysis of Tea Bioactive Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Catechins, theanine and caffeine were analyzed on a Waters High Performance Liquid Chromatography (HPLC) system equipped with Waters 600 controller and Waters 2489 UV Detector (280 nm). The Empower ^TM 3 software was used for instrument operation control and data collection. Chromatographic separation was performed on a Gemini 5u C18 110A column (250×4.60 mm, 5 μm, Phenomenex Inc., Torrance, CA), with a solvent system consisting of 0.17% (v/v) acetic acid (A) and 100% acetonitrile (B); a linear gradient at a flow rate of 1.0 mL/min was set as follows: B from 8 to 28% (v/v) in 30 min was initiated, followed by B from 28 to 100% (v/v) between 30 and 37 min, and B from 100 to 8% (v/v) between 37 and 46 min. Peaks were identified by comparison of retention times with those of standards49 (link). Total sugar content of tea infusion was determined by the anthrone-sulfuric acid method50 (link). Polysaccharides were isolated from tea infusion using an Amicon Ultra-0.5 Centrifugal Filter Device (UFS 503024, 3,000 Dalton cutoff).

Han M., Zhao G., Wang Y., Wang D., Sun F., Ning J., Wan X, & Zhang J. (2016). Safety and anti-hyperglycemic efficacy of various tea types in mice. Scientific Reports, 6, 31703.

+ Open protocol

+ Expand

Quantitative Dopamine Detection by HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dopamine was quantitatively detected by HPLC (Waters Instruments, Inc., Rochester, MN). Chromatographic separation was performed on a Gemini 5 u C18 110 A column (250 × 4.60 mm, Phenomenex Inc., Torrance, CA, USA). The separation conditions were as follows: the column temperature was maintained at 30 °C, the flow rate was 1 mL/min, the single injection volume was 10 μL, and the mobile phases were (A) 0.15% acetic acid–water (ultra-pure water plus 0.15% acetic acid) and (B) acetonitrile. A gradient elution separation method was set, the total detection time was 40 min, and phase A was taken as an example: (1) Initial mobile phase proportion A: 85%, keep running for 10 min; (2) switch to another gradient elution A: 77%, run for 15 min; (3) reduce to A: 71% within 1 min; (4) convert A: 0% at the 26th min and maintain for 4 min; (5) return to initial state A: 85% within 2 min and maintain for 8 min; (6) switch to A: 80% at 40 min and maintain the balance system for the next injection. The amount of dopamine in the eluent was measured by the absorbance with wavelengths of 278 nm.

Wang W., Wu X., Yang C.S, & Zhang J. (2021). An Unrecognized Fundamental Relationship between Neurotransmitters: Glutamate Protects against Catecholamine Oxidation. Antioxidants, 10(10), 1564.

+ Open protocol

+ Expand

Lipidomic Analysis of Bronchoalveolar Lavage

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reversed phase liquid chromatography (LC) and MS was performed on an AB Sciex API 3200 triple quadrupole mass spectrometer with an electrospray ionization source (AB Sciex, Concord, Canada). Chromatography was performed on a Shimadzu LC20-AD HPLC system equipped with a Gemini 5u C18 110A column (150×2.00 mm, 5 um, Phenomenex). For acquisition of full scan data, the gradient mobile phase was composed of A: 60/20/20 of methanol/acetonitrile/water v/v/v with 2 mM ammonium acetate and B: methanol with 2 mM ammonium acetate. The flow rate was 0.2 mL/min. Initial conditions was 40% A for 1 min, followed by a linear gradient from 40 to 100% B within 50 min, 100% B was then held for 5 min, followed by re-equilibration for 8 min. Each sample was injected in duplicate in order to improve the statistical analysis.
For untargeted analysis of lipids in BAL samples, mass spectra were acquired in full scan mode. Full scans were carried out in both positive and negative mode where a range of m/z 400–1000 was employed. The orifice was set at +58 and −50 V in positive and negative mode, respectively. Data acquisition was carried out by Analyst software 1.6.1.

Almstrand A.C., Voelker D, & Murphy R.C. (2015). Identification of oxidized phospholipids in bronchoalveolar lavage exposed to low ozone levels using multivariate analysis. Analytical biochemistry, 474, 50-58.

+ Open protocol

+ Expand

HPLC-based Determination of Formaldehyde in Mice Exposure

Check if the same lab product or an alternative is used in the 5 most similar protocols

The separation and determination of FA-2, a 4-DNPH derivative were performed on an LC-20A HPLC system (Shimadzu, Kyoto, Japan) equipped with binary LC-20AD pumps, a DGU-20A₃ degasser, a SPD-20A ultraviolet detector, a SIL-20A autosampler, and a CTO-20AC column oven. A Gemini 5u C18 110A column (150 mm 4.6 mm I.D., 5 μm) (Phenomenex, Torrance, CA, USA) was used as an analytical column. The mobile phase of acetonitrile-distilled water (60:40, v/v) was used. The flow rate was 1.0 mL/min. The column temperature was 40 °C and the injection volume was 10 μL. The analyte was monitored at the wavelength of 360 nm. Quantitation was performed using synthesised FA-2, 4-DNPH solution (Sigma-Aldrich Co., St. Louis, MO, USA) as standard. Each group of ten mice was exposed to 0 (control), 1.38 ± 0.20 mg/m³ (mean ± SD), or 5.36 ± 0.52 mg/m³ FA for 4 h a day and 5 days a week over a 2-week period (Fig. 7).Figure 7

Concentrations of FA. The (A) mean and (B) daily FA concentrations were monitored using a 2, 4-DNPH cartridge and HPLC–UV. Female BALB/c mice were exposed to two different concentrations (1.38 mg/m³ and 5.36 mg/m³) of FA for 4 h/day and 5 days/week, for 2 weeks. Data are presented as means ± SD.

Park J., Yang H.S., Song M.K., Kim D.I, & Lee K. (2020). Formaldehyde exposure induces regulatory T cell-mediated immunosuppression via calcineurin-NFAT signalling pathway. Scientific Reports, 10, 17023.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!