Film explant samples were separated from the surrounding peritoneal tissue, weighed, and dried at 37˚C for 4 days. Dried explant samples at 4 and 10 weeks were pooled, converted into fatty acid methyl esters (FAMEs) through trans-esterification and their separation by liquid-liquid extraction and compared to non-implanted film controls. Analysis of the FAMEs was performed on the Trace Ultra Gas Chromatograph with TriPlus Autosampler and FID detector from Thermo Fischer Scientific with separation using the FAMEWAX® Polyethylene glycol (USP G16) phase capillary GC column (30m length × 0.32 mm ID × 0.25 μm film) from Restek. The column temperature was programmed from 195°C to 240°C at 1.7°C/min. The injections were performed with split ratio 100:1 and constant flow operating mode at 1 mL/min (helium used as carrier gas). Flame gases were 350 mL air/min, 35 mL hydrogen/min, and 30 mL Makeup/min. The inlet and detector temperatures were both set at 250°C. The injection volume was 1 μL.
Triplus autosampler
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
The TriPlus autosampler is a laboratory instrument designed to automate the sampling and introduction of liquid or solid samples into an analytical instrument, such as a gas chromatograph or a liquid chromatograph. The core function of the TriPlus autosampler is to precisely and consistently deliver samples for analysis, ensuring reproducible and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Trace gc ultra gas chromatograph, by Thermo Fisher Scientific (2 mentions)
Tsq quantum xls triple quadrupole mass spectrometer, by Thermo Fisher Scientific (2 mentions)
Trace ultra gas chromatograph, by Thermo Fisher Scientific (1 mentions)
Rtx 5ms capillary column, by Restek (1 mentions)
7890a 5975c gc ms system, by Agilent Technologies (1 mentions)
Hp 5ms capillary column, by Agilent Technologies (1 mentions)
Delta 5 advantage, by Thermo Fisher Scientific (1 mentions)
Gc isolink, by Thermo Fisher Scientific (1 mentions)
Delta 5 plus irms, by Thermo Fisher Scientific (1 mentions)
Conflo 3, by Thermo Fisher Scientific (1 mentions)
J w db wax, by Agilent Technologies (1 mentions)
Xcalibur software, by Thermo Fisher Scientific (1 mentions)
Tsq8000 detector, by Thermo Fisher Scientific (1 mentions)
Guard column, by Restek (1 mentions)
Thermo xcalibur, by Thermo Fisher Scientific (1 mentions)
Trace gc ultra gas chromatographer, by Thermo Fisher Scientific (1 mentions)
Tsq quantum gc mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Trace gc ultra, by Thermo Fisher Scientific (1 mentions)
Db ffap, by Agilent Technologies (1 mentions)
Itq 900, by Thermo Fisher Scientific (1 mentions)
15 protocols using triplus autosampler
1
Fatty Acid Profiling of Implant Samples
2
Headspace-SPME-GC-MS Analysis of Coffee Volatiles
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Each coffee ground sample was then analyzed by HS–SPME–GC–MS using a CAR/PDMS fiber (100 μm) and a Rtx-5MS capillary column (30 m × 0.25 mm i.d., 0.25 µm film thickness; Restek Corporation, Bellefonte, PA, USA). GC–MS analysis was carried out using a TraceGQ Ultra (Thermo-Fisher Scientific, San Jose, CA, USA) coupled with an HS-SPME system (Triplus autosampler, Thermo-Fisher) and single quadrupole mass spectrometry (ISQ, ThermoFisher). Helium was the carrier gas with a column head pressure of 55 kPa. The conditions used for the HS-SPME study were as follows: incubation time 15 min, temperature 60 °C, extraction time 15 min and desorption time 6 min. The oven program was 40 °C for 5 min, with a rate of 5 °C/min up to 190 °C, maintained 8 min and up to 240 °C with a rate of 10 °C/min, maintained 10 min. The acquisition was in SCAN mode (35–350 m/z). The putative identification of volatile compounds was carried out using the NIST Chemistry WebBook spectrum library present in the equipment software.
3
GC-MS Analysis of Derivatized Samples
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Analysis was performed on an 7890A-5975C GC-MS system (Agilent Technologies, Santa Clara, CA, USA) equipped with an HP-5MS capillary column (30 m × 0.25 mm × 0.25 μm) (Agilent J &W Scientific, Folsom, CA). All the samples and replicates were continuously injected as one batch in random order to discriminate technical from biological variations. Additionally, the prepared pooled samples were used as quality controls (QCs), which were injected at regular intervals (every ten samples) throughout the analytical run to provide a set of data from which the repeatability can be assessed.
Each 1 μL aliquot of the derivatized sample solution was injected in splitless into the GC column by a TriPlus autosampler (Thermo Fisher Scientific), at a constant flow rate of helium of 1 mL·min−1. The temperature of the injector was operated isothermally at 280 °C. The mass spectrometry (MS) transfer line temperature to the quadruple was set to 150 °C and the electron impact (EI) ion source ion temperature was 230 °C. Compound elution settings were 2min at 80 °C isothermal, followed by a 10 °C∙min−1 oven temperature gradient to a final 325 °C, and then hold for 6 min at 325 °C. The system is then temperature equilibrated for 1 min at 80 °C before injecting the next sample. Ions were generated by a 70 eV electron beam. The spectra were recorded with a scanning range of 50–550 m ∙ z−1.
Each 1 μL aliquot of the derivatized sample solution was injected in splitless into the GC column by a TriPlus autosampler (Thermo Fisher Scientific), at a constant flow rate of helium of 1 mL·min−1. The temperature of the injector was operated isothermally at 280 °C. The mass spectrometry (MS) transfer line temperature to the quadruple was set to 150 °C and the electron impact (EI) ion source ion temperature was 230 °C. Compound elution settings were 2min at 80 °C isothermal, followed by a 10 °C∙min−1 oven temperature gradient to a final 325 °C, and then hold for 6 min at 325 °C. The system is then temperature equilibrated for 1 min at 80 °C before injecting the next sample. Ions were generated by a 70 eV electron beam. The spectra were recorded with a scanning range of 50–550 m ∙ z−1.
4
Optimization of SPME-GC/MS Analysis for Chimonanthus praecox Floral Fragrance
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The optimization of SPME-GC/MS based on the methods of Chimonanthus praecox was performed in our study [18 (link)]. A 100 μm PDMS SPME fiber (American Supelco Company) was combined with an SPME automatic sampler device for HS-SPME analysis. After the sample was equilibrated at room temperature for 30 min, the floral fragrance substances of the P. notoginseng flower were adsorbed into the extraction fiber. Before sampling the floral fragrance substance, the GC-MS inlet of the SPME fiber was adjusted to 250 °C for 40 min. The fiber was then inserted into the top of a sealed SPME vial using an SPME autosampler (fiber conditioning temperature of 250 °C, 20 min, TriPlus autosampler, Thermo Fisher Scientific), and the sample was adsorbed at room temperature for 40 min.
5
GC-MS Analysis of Derivatized Samples
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In GC–MS analysis, the extracted samples were derivatised with trimethylsilyl (TMS) as described by Chandra and Kumar (2017 (link)). An aliquot (2.0 µL) of derivatised sample was injected in GC–MS (Trace GC Ultra Gas Chromatograph, Thermo Scientific, USA) equipped with a TriPlus auto sampler coupled to TSQ Quantum XLS triple quadrupole mass spectrometer (Thermo Scientific, USA). Separation was carried out on DB-5 MS capillary column (30-m length × 0.25 µm I.D. × 0.25 mm film thickness of 5% phenyl and 95% methylpolysiloxane) with helium as the carrier gas at a flow rate of 1.1 mL min−1. The temperature of GC oven was programmed started from 65 °C (hold for 2 min), increased to 230 °C at a rate of 6 °C min−1 and finally reached to 290 °C (hold for 20 min) at a rate of 10 °C min−1 increased. The transfer line temperature and ion source temperature were kept at 290 and 220 °C, respectively. The mass spectrum (MS) was operated in the positive electron ionization (+ EI) mode at an electron energy of 70 eV with a solvent delay of 7 min. The MS was operated in full-scan mode from m/z, 45–800. The detected organic compounds were identified by matching with the MS library NIST v. 1.0.0.12 available with instrument.
6
Determining 13C Enrichment in Biomarkers
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To determine the 13C enrichment of biomarkers, all raw δ13C were measured individually for AS and PLFA using a Delta V Advantage isotope ratio mass spectrometer via a ConFlo III interface (Thermo Fisher Scientific, Bremen, Germany). For AA, all raw δ13C were measured using a trace GC Ultra mounted with a TriPlus autosampler (Thermo Scientific, Hvidovre, Denmark) coupled via a combustion reactor (GC IsoLink, Thermo Scientific) to an isotope ratio mass spectrometer (Delta V Plus IRMS, Thermo Scientific). For each sample, chromatogram peaks identified based on retention times specific for the measured amino sugars, PLFA, and AA were integrated using Isodat v. 3.0 (Thermo Fisher Scientific). All raw δ13C values were corrected for dilution by additional C atoms added during the derivatization, amount dependence, offset, and drift (for PLFA samples)49 (link)–51 (link). To determine the 13C incorporation into each biomarker, the 13C excess for each biomarker as determined by the difference between the 13C of the labeled and unlabeled biomarker was multiplied by the C content of the specific biomarker.
7
GC-MS Analysis of Derivatized Samples
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The derivatization of the extracted samples was carried out using trimethylsilyl (TMS). GC–MS (Trace GC Ultra Gas Chromatograph, Thermo Scientific, USA) equipped with a TriPlus auto sampler coupled to TSQ Quantum XLS triple quadrupole mass spectrometer (Thermo Scientific, USA) was injected with 2.0 µL aliquot of the derivatised sample. DB-5 MS capillary column (30-m length × 0.25 µm I.D. × 0.25 mm film thickness of 5% phenyl and 95% methylpolysiloxane) was used for the separation process. Helium was the carrier gas with a flow rate of 1.1 mL min−1. The temperature for the GC oven varied starting at 65 °C (hold for 2 min) to gradually increasing up to 230 °C at the rate of 6 °C min−1. The final temperature recorded, reached to 290 °C (hold for 20 min) at a rate of 10 °C min−1 high.
For the mass spectrum the positive electron ionization (+ EI) mode at electron energy of 70 eV with a solvent hold-up of 7 min was followed for the operation. A full-scan mode from m/z, 45–800 was used for the operation of mass spectrum. MS library NIST v. 1.0.0.12 was used to detect and identify the organic compounds.
For the mass spectrum the positive electron ionization (+ EI) mode at electron energy of 70 eV with a solvent hold-up of 7 min was followed for the operation. A full-scan mode from m/z, 45–800 was used for the operation of mass spectrum. MS library NIST v. 1.0.0.12 was used to detect and identify the organic compounds.
8
Soil Chemical Properties and PAHs Analysis
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Selected chemical properties of soil (pH, Hh, total N, Corg) were analyzed. The following parameters were determined in air-dried soil samples: pH 1 mol KCl∙dm−3, by the potentiometric method; hydrolytic acidity (Hh), by Kappen’s method; total nitrogen content, by distillation after mineralization in sulfuric (VI) acid with the addition of the selenium reagent mixture; organic carbon content, by the Kurmies method.
The content of 16 PAHs was determined with the Trace GC/MS Ultra ITQ900 system with a TRIPlus autosampler (Thermo Fisher Scientific, Waltham, MA, USA) and a flame ionization detector. The total content of 16 PAHs (naphthalene, acenaphthene, acenaphthylene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, indeno(1,2,3-cd)pyrene, dibenzo(a,h)anthracene and benzo(g,h,i)perylene) was determined by the method described by Krzebietke et al. [3 (link)]. The content of LMW PAHs (naphthalene, acenaphthene, acenaphthylene, fluorene, anthracene, phenanthrene, fluoranthene, pyrene and chrysene) and HMW PAHs (benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(g,h,i)perylene, indeno(1,2,3-cd)pyrene and dibenzo(a,h)anthracene) was determined in soil samples.
The content of 16 PAHs was determined with the Trace GC/MS Ultra ITQ900 system with a TRIPlus autosampler (Thermo Fisher Scientific, Waltham, MA, USA) and a flame ionization detector. The total content of 16 PAHs (naphthalene, acenaphthene, acenaphthylene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, indeno(1,2,3-cd)pyrene, dibenzo(a,h)anthracene and benzo(g,h,i)perylene) was determined by the method described by Krzebietke et al. [3 (link)]. The content of LMW PAHs (naphthalene, acenaphthene, acenaphthylene, fluorene, anthracene, phenanthrene, fluoranthene, pyrene and chrysene) and HMW PAHs (benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(g,h,i)perylene, indeno(1,2,3-cd)pyrene and dibenzo(a,h)anthracene) was determined in soil samples.
9
High-resolution GC-HRMS for PCDD/Fs and DL-PCBs
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High-resolution gas chromatography coupled with high-resolution mass spectrometry was used for detection and the apparatus was an Ultra Trace GC gas chromatograph, TriPlus autosampler, and DFS dual-focusing mass spectrometer (Thermo Scientific, Bremen, Germany). Positive electron ionisation operating in selected-ion monitoring mode at a resolution of 10,000 was employed. Chromatographic separation was carried out in a DB-5 MS fused-silica capillary column (60 m × 0.25 mm × 0.1 mm). The limits of quantification (LOQs) were 0.01–0.12 pg g−1 w.w. for PCDD/Fs and 0.09–1.16 pg g−1 w.w for DL-PCBs. Estimation of the LOQs of individual congeners was performed in accordance with the European Commission Joint Research Centre’s Guidance Document on the Estimation of LOD and LOQ for Measurements in the Field of Contaminants in Feed and Food (11 (link)).
10
Quantification of 4MMP in Wine Samples
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The 4MMP concentration in samples was quantified using a method published by Dagan et al. (2014 (link)), which was optimized for the use of triple quadrupole. The 4MMP was derivatized directly in the wine sample for 45 min at 55 °C using EDTA, l -cysteine and O-methylhydroxylamine hydrochloride and then extracted by SPME for 30 min at 55 °C on a DVB/CAR/PDMS fiber previously conditioned at 250 °C for 12 min. Finally, the compounds were desorbed into the GC inlet at 250 °C for 3 min. Derivatized compounds were separated, identified and quantified using a Trace Ultra gas chromatograph (GC) equipped with a Triplus Autosampler and coupled with a triple quadrupole mass spectrometer TSQ 8000 detector from ThermoScientific (Austin, Texas, USA). Analysis and data treatment were monitored using the Xcalibur software (ThermoScientific, Austin, Texas, USA).
The GC was equipped with a J&W DB-WAX (60 m × 0.25 mm × 0.25 µm) column (Agilent, Santa Clara, California, USA). The carrier gas was helium with a constant flow rate of 1.2 mL/min, and the injector temperature was set at 250 °C. Injection was performed in splitless mode for 3 min and then operated at a split of 1/20. The source and transfer line temperatures were set at 250 °C. Ionization was performed by positive electronic impact (EI) at 70 eV, with argon being used for the second fragmentation.
The GC was equipped with a J&W DB-WAX (60 m × 0.25 mm × 0.25 µm) column (Agilent, Santa Clara, California, USA). The carrier gas was helium with a constant flow rate of 1.2 mL/min, and the injector temperature was set at 250 °C. Injection was performed in splitless mode for 3 min and then operated at a split of 1/20. The source and transfer line temperatures were set at 250 °C. Ionization was performed by positive electronic impact (EI) at 70 eV, with argon being used for the second fragmentation.
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