For whole-body or whole-tumor imaging, an Olympus small animal imaging system, OV-100, was used. The OV100 small animal imaging system (Olympus Corp., Tokyo, Japan), was used. The OV100 contains an MT-20 light source (Olympus Biosystems, Planegg, Germany) and DP70 CCD camera (Olympus), for whole body, as well as subcellular imaging in live mice [26 (link)–29 (link)]. The optics of the OV100 have been specially developed for macroimaging as well as microimaging with high light-gathering capacity. Four individually optimized objective lenses, parcentered and parfocal, provide a 105-fold magnification range. High-resolution images were captured directly on a PC (Fujitsu Siemens, Munich, Germany). Images were processed for contrast and brightness and analyzed with the use of Paint Shop Pro 8 and CellR [30 (link)].
Ov100
Manufactured by Olympus
Sourced in Japan
The OV100 is a compact, highly sensitive, and versatile imaging system designed for a wide range of laboratory applications. The system features high-resolution optics, advanced imaging sensors, and powerful software to capture detailed images and videos of biological samples.
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Lab products found in correlation
Ov100 small animal imaging system, by Olympus (2 mentions)
Iv100, by Olympus (2 mentions)
Vs120 fl, by Olympus (2 mentions)
Fv1000 confocal microscope, by Olympus (2 mentions)
Dp70 ccd camera, by Olympus (1 mentions)
Mt 20 light source, by Olympus (1 mentions)
Wasabi software, by Hamamatsu Photonics (1 mentions)
Tissue tek oct compound, by Sakura Finetek (1 mentions)
Rompun, by Bayer (1 mentions)
Axioskop 2 fs plus, by Zeiss (1 mentions)
Cm1900 microtome, by Leica (1 mentions)
Balb c mice, by Japan SLC (1 mentions)
X cite exacte, by Excelitas (1 mentions)
Orca r2, by Hamamatsu Photonics (1 mentions)
Ix81 zdc, by Olympus (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
Image pro plus software, by Media Cybernetics (1 mentions)
18 protocols using ov100
1
Small Animal Whole-Body Imaging with OV100
2
Tumor Extraction and Imaging
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Tissues from subcutaneous tumors and normal organs (blood, spleen and liver) were removed at termination from the nude mice with subcutaneous tumors. S. typhimurium A1-R was extracted from the tumors and organs and cultured in LB agar for 24 hours, and imaged with the Olympus OV100.
3
Biodistribution of BNC-LP complexes
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Mice were handled according to the guidelines of Graduate School of Bioagricultural Sciences, Nagoya University, Japan. Animal experiments described in this study were approved by the animal experiment committee in Nagoya University (approved number 2010031805). Each female Balb/c mouse (6 weeks old, CREA Japan, Tokyo, Japan) was injected intravenously with 50 µL of PBS containing 10 µg (as protein) of BNC-LP complexes. After 30, 180, and 300 min, Rh-derived whole-body fluorescent signals were measured by an in vivo imaging system OV-100 (Olympus, Tokyo, Japan) equipped with a xenon lamp and emission filters (from 535–555 nm) and analyzed using WASABI software (Hamamatsu Photonics, Shizuoka, Japan).
4
Systemic S. typhimurium A1-R Delivery in Tumor-Bearing Mice
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SKOV3-GFP (5×106 cells) were injected i.p. in 10 nude mice. Fourteen days after the implantation, five mice were treated with i.v. injection of S. typhimurium A1-R (5×107 CFU) and another five mice were treated with i.p. injection of S. typhimurium A1-R (5×107 CFU). Ten nude mice without tumor were also treated with S. typhimurium A1-R i.v. or i.p., five mice each (Fig. 4a ). Twenty-four hours after bacterial administration, blood, ascites, liver, spleen and tumor were harvested and seeded in LB-Agar dishes. After 24-hour culture, S. typhimurium A1-R colony formation was assessed with fluorescence imaging (OV100, Olympus, Japan) (Fig. 4b, c ).
5
Multimodal Imaging for Cell and Tissue Analysis
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Microscopic imaging was performed using a fluorescence reflectance imaging system (OV100; Olympus Corporation, Tokyo, Japan) and a multi-wavelength laser scanning microscope (IV100; Olympus) to acquire wide-field and high-power images, respectively [18 (link)]. Live cells and tissue sections were scanned using a fluorescence virtual microscopy system (VS120-FL; Olympus) [18 (link)]. Endoscopic imaging was performed using an in-house endoscopic system, as described previously [14 (link)]. ICG fluorescence was excited by a xenon light source through a 600 ± 200-nm band-pass filter and detected by a sensitive electron-multiplying charge-coupled device camera (MC285SPD-L0B0; Texas Instruments, Dallas, TX, USA) through a 842.5 ± 17.5-nm band-pass filter. As a flexible endoscope, a bronchoscope fiberscope (BF-XP60; Olympus Medical Corporation, Tokyo, Japan), 2.8 mm in diameter with a single biopsy channel, was used. Fluorescence images were shown in grayscale or pseudo-colors.
6
Lymphatic Tracking of Nanoparticles
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The mice were anesthetized intraperitoneally using a solution containing 8 mg/mL ketamine (Ketalar®, Panpharma, Fougères, France) and 0.8 mg/mL xylazine (Rompun®, Bayer Pharma, Puteaux, France) at 0.015 mL/g of body weight. The lymph node was examined after intradermal injection into the left forepaw pad with the DTX/FPR-675 Pluronic NPs in an aqueous dispersion (10 μL, 1 mg/mL of DTX) using an NIRF image system. Images for lymphatic tracking were obtained at predetermined time points after NP injection using a 580 nm filtered charge coupled device camera under illumination at 460–490 nm, which was implemented in a high sensitivity imaging system (Olympus OV100) for data acquisition and analysis. In order to evaluate the distribution of NPs in lymphatic tissue, the lymph node (brachial) was obtained from normal mice and tumor-bearing mice and the obtained tissue was embedded in a Tissue Tek® OCT compound (Sakura Finetek, Torrance, CA, USA) and cryosectioned at 8 μm (CM1900 microtome, Leica). The fluorescent images were observed under a fluorescence microscope (Olympus BX51 and Zeiss Axioskop2 FS Plus).
7
Biodistribution of CF750-labeled α-DC-ZZ-BNC
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CF750-labeled α-DC-ZZ-BNC (10 μg as ZZ-L protein) was injected to Balb/c mice (6–8 weeks, female; Japan SLC, Inc.) through SC or IM routes. After 12 h, the mice were sacrificed, and tissues were isolated. CF750-derived fluorescence in each tissue was observed by using an in vivo imaging system OV-100 (Olympus), and then semi-quantified with an image processing program FV10-ASW version 4 (Olympus).
8
Longitudinal Imaging of Pancreatic Tumors
9
Fluorescence-Guided Liver Metastasis Resection
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Four weeks after SOI of HT-29-GFP to the liver, the liver metastasis was exposed and imaged preoperatively with the OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) [16 (link)] at a magnification of 0.14x. Fourteen mice underwent surgery: Fluorescence-guided surgery (FGS) in 7 mice and bright-light surgery (BLS) in 7 mice (Fig 2A and 2B ). FGS was performed using the Dino-Lite imaging system (AM4113TGFBW Dino-Lite Premier; AnMo Electronics Corporation, New Taiwan). The surgical resection bed was imaged with the Olympus OV100 at a magnification of 0.14x or 0.56x to detect microscopic residual cancer. Residual tumor area was analyzed with ImageJ v1.49f (National Institutes of Health). The incision was closed in one layer using 6–0 nylon surgical sutures.
10
Fluorescence Imaging System Specifications
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The basic specifications of fluorescence imaging systems has been reported previously.24 Briefly, macroscopic images were captured using a fluorescence reflectance imaging system (OV100; Olympus, Tokyo, Japan) equipped with a 150 W xenon light source and a color digital CCD camera (DP71; Olympus). Excitation and emission filters for ICG fluorescence imaging were 730 ± 22.5‐nm band‐pass and 770‐nm long‐pass filter, respectively. High‐power view was observed using a multi‐wavelength laser scanning microscope (IV100; Olympus) equipped with a 748‐nm diode laser with 17 mW power output and a photomultiplier tube. The emission filter to detect ICG fluorescence was a 779‐nm long‐pass filter. Tissue sections were scanned using a fluorescence virtual microscopy system (VS120‐FL; Olympus) equipped with a 200 W mercury light source (X‐Cite exacte; Excelitas Technologies, Waltham, MA, USA) and a monochrome digital CCD camera (ORCA‐R2; Hamamatsu Photonics, Hamamatsu, Japan). Excitation and emission filters for ICG fluorescence were 708 ± 37.5‐nm band‐pass and 809 ± 40.5‐nm band‐pass filter, respectively. Fluorescence images are shown in grayscale or pseudo‐colors.
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