Apigenin

Manufactured by Chengdu Must Bio-Technology

Sourced in China

Apigenin is a naturally occurring flavonoid found in various plants. It serves as a core compound in research and development applications within the life sciences industry. As a laboratory reagent, Apigenin is used to facilitate specific experiments and analyses, providing a reliable and consistent source material for researchers and scientists.

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Lab products found in correlation

12 protocols using apigenin

Quantification of Bioactive Compounds in Lanqin Oral Liquid

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Lanqin oral liquid (lot no. 19122821) was purchased from Yangtze River Pharmaceutical Group (Jiangsu, China). Reference compounds (purity > 98%) of chlorogenic acid, caffeic acid, scutellarin, baicalin, palmatine, wogonoside, apigenin, baicalein, wogonin, indirubin, and indigo were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). Acetonitrile and formic acid of HPLC grade were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Purified water was purchased from Hangzhou Wahaha Corporation (Hangzhou, China). All other chemical reagents used in this study were of analytical grade.

Liu T, & Lin S. (2023). Systematic Screening of the Chemical Constituents of Lanqin Oral Liquid by Ultra-High-Performance Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Molecules, 28(20), 7053.

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Purchasing and Characterizing Natural Products

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Quercetin, scutellarin, cynaroside, naringin, hesperidin, diosmetin, luteolin, hesperitin, apigenin, naringenin, rutin, wogonin, and sophoricoside were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). Baicalein was purchased from Solarbio (Beijing, China). The purity of all the natural products was ≥98%. The reference standard of adenosine (purity >99%) was purchased from Tokyo Chemical Industry (Shanghai, China). M⁶A (purity 97%) was purchased from J&K Chemical (Shanghai, China).

Du Y., Yuan Y., Xu L., Zhao F., Wang W., Xu Y, & Tian X. (2022). Discovery of METTL3 Small Molecule Inhibitors by Virtual Screening of Natural Products. Frontiers in Pharmacology, 13, 878135.

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Flavonoid Standards Acquisition Protocol

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Kaempferide and apigenin were purchased from Chengdu Must Bio-technology Co., Ltd (Chengdu, China). Naringenin, Gallic acid, benzoic acid, caffeic acid, ferulic acid, sinapic acid, cyanidin, catechins, UDP-glucose, and UDP-galactose were obtained from Sigma (St Louis, MO, USA). Quercetin 3-O- galactoside, kaempferol 3-O- galactoside, kaempferol 3-O-glucoside, kaempferol 7-O-glucoside, myricetin 3-O-galactoside, quercetin, kaempferol, myricetin and genistein were purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China).

Dai X., Zhuang J., Wu Y., Wang P., Zhao G., Liu Y., Jiang X., Gao L, & Xia T. (2017). Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis). Scientific Reports, 7, 5926.

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Anti-inflammatory Compounds from E. coli LPS

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Lipopolysaccharides (LPSs) from E. coli 055:B5 were purchased from Solarbio (Cat. No. L8880, Beijing, China) with a purity > 99%. N-Acetyl-l-cysteine (NAC) and vitamin C (VC) were purchased from Beyotime (Cat. No. ST1546; Cat. No. ST1434, Shanghai, China) with a purity > 99%. The standard samples of apigenin, quercetin, chrysin and diosmetin were purchased from CHENGDU MUST Biotechnology CO.,LTD (Cat. No. A0113, A0083, A0292, A0927, MUST, Chengdu, China) with HPLC purity > 99%. Rabbit BRCA1 Polyclonal Antibody was purchased from Beyotime (Cat. No. AF6339, Shanghai, China). BeyoClick EdU 488 kit was purchased from Beyotime (Cat. No. C0071S, Shanghai, China).

Xi X., Wang J., Qin Y., You Y., Huang W, & Zhan J. (2022). The Biphasic Effect of Flavonoids on Oxidative Stress and Cell Proliferation in Breast Cancer Cells. Antioxidants, 11(4), 622.

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Evaluating Antiviral Compounds Against BVDV-1a in Mice

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BALB/c mice were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. (Beijing, China) (6–8 weeks old, 18–22 g). The cp BVDV-1a (strain NADL, No. VR-534). Daidzein, artemisinine, apigenin, and curcumin were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China) (Table S2). and was resuspended in DMSO. CCK8 cell activity assay kit was purchased from Shanghai Biyuntian Biotechnology Co (Shanghai, China).

Chen N., Jiang D., Shao B., Bai T., Chen J., Liu Y., Zhang Z., Zhou Y., Wang X, & Zhu Z. (2023). Anti-BVDV Activity of Traditional Chinese Medicine Monomers Targeting NS5B (RNA-Dependent RNA Polymerase) In Vitro and In Vivo. Molecules, 28(8), 3413.

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Phytochemical Analysis of Lianhua Qingwen

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Lianhua Qingwen capsule (Lot No. B2008172) were obtained from Yiling Pharmaceutical Co., Ltd. (Shijiazhuang, China). Reference compounds (purity>98 %) of chlorogenic acid, esculetin, caffeic acid, luteolin, apigenin, liquiritigenin, isoliquiritigenin, liquiritin, isoliquiritin, glycyrrhizic acid and glycyrrhetinic acid were purchased from Chengdu Must Bio-Technology Co., Ltd (Chengdu, China). Acetonitrile and formic acid of HPLC grade were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Purified water was provided from Hangzhou Wahaha Corporation (Hangzhou, China).

Liu T, & Lin S. (2024). Comprehensive characterization of the chemical constituents of Lianhua Qingwen capsule by ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry. Heliyon, 10(6), e27352.

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HPLC Analysis of EEAI Composition

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HPLC analysis was employed to determine the composition of EEAI. The eluted fractions were characterized based on their retention time in comparison to the reference standard ovatodiolide (ARJIL Pharmaceuticals LLC Ltd., Hsinchu, Taiwan), anisomelic acid (ARJIL Pharmaceuticals LLC Ltd., Hsinchu, Taiwan), and apigenin (Chengdu Must Bio-Technology Co., Ltd., Chendu, China). The constituents were characterized by utilizing a photodiode array detector and comparing them with standard UV spectra at a wavelength of 220 nm. A TSK gel Tosoh ODS-80Tm column (250 × 4.6 mm i.d., 5 µm) (Tosoh, Yamaguchi, Japan) in reversed phase was employed for compound separation. During the interval of 0–8 min, a mixture of acetonitrile (J.T Baker, Phillipsburg, NJ, USA) and 0.1% acetic acid (Cascina Favaglie, Milan, Italy) in water (64:36) was used as the mobile phase while keeping the flow rate consistent at 1 mL/min [49 (link)].

Chen Y.R., Jiang W.P., Deng J.S., Chou Y.N., Wu Y.B., Liang H.J., Lin J.G, & Huang G.J. (2023). Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro. International Journal of Molecular Sciences, 24(20), 15062.

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HPLC Analysis of Flavonoid Compounds

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Flavonoid samples used in this study were all purchased from companies. Jaceosidin, hispidulin and homoplantaginin were purchased from Sichuan Weikeqi-biotech company, China. Luteolin and Apigenin were purchased from Chengdu Must Bio-technology company, China. Rutin were purchased from Tianjin Shilan technology company, China. All these compounds were detected by HPLC (SIL-20A, Shimadzu, Japan), chromatographic column: Dikma Diamonsil C₁₈ column (250 mm × 4.6 mm i.d., 5 μM). The methods of HPLC for jaceosidin, hispidulin, homoplantaginin, Luteolin and Apigenin are as follow: 30% acetonitrile, 70% water (0.1% phosphoric acid), 0 min; 60% acetonitrile, 40% water (0.1% phosphoric acid), 30 min. The method for rutin is as follow: 35% acetonitrile, 65% water (0.1% phosphoric acid), 30 min. All samples were dissolved in methanol respectively. The HPLC chromatogram of the flavonoid samples were provided in supplementary material (Fig. S1). The purity of all the compound samples was more than 98%.

Guo Q.R., Zhou W.M., Zhang G.B., Deng Z.F., Chen X.Z., Sun F.Y., Lei X.P., Yan Y.Y, & Zhang J.Y. (2023). Jaceosidin inhibits the progression and metastasis of NSCLC by regulating miR-34c-3p/Integrin α2β1 axis. Heliyon, 9(5), e16158.

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Antioxidant Activity of Olive Leaf Extracts

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The Manzanillo variety of olive trees was cultivated in the commercial olive orchards of Xichang, Sichuan. Olive leaves were collected in April and July, 2017. The leaves were washed and dried at 45 °C, and then ground into powder by a high speed mill (FW177, Taisite Instrument Co., Ltd., Tinjin, China). The powder was stored at −20 °C.
Phenazine methosulfate (PMS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), nicotinamide adenine dinucleotide (NADH), and nitroblue tetrazolium (NBT) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Fetal bovine serum (FBS) and penicillin–streptomycin were from Gibco/BRL (Burlington, Canada). HPLC-grade apigenin-7-O-β-d-glucosidase, apigenin, quercetin, rutin, luteolin, and luteolin-4’-O-glucoside were purchased from the Chengdu Must Biotechnology Co., LTD (Chengdu, China); acetonitrile was purchased from Acros Organics (Geel, Belgium). All other reagents were analytical grade and obtained from Chengdu Kelong Chemical Factory (Chengdu, China).

Wang B., Qu J., Luo S., Feng S., Li T., Yuan M., Huang Y., Liao J., Yang R, & Ding C. (2018). Optimization of Ultrasound-Assisted Extraction of Flavonoids from Olive (Olea europaea) Leaves, and Evaluation of Their Antioxidant and Anticancer Activities. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(10), 2513.

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Quantitative Analysis of Bioactive Compounds

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Reference standards including chlorogenic acid, caftaric acid, caffeic acid, cichoric acid, luteolin, luteolin-7-O-β-d-glucoside, isochlorogenic acid A, isochlorogenic acid C, apigenin and quercetin were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). DPPH (1,1-Diphenyl-2-picrylhydrazyl), copper (II) chloride, ascorbic acid, sodium acetate trihydrate, iron (III) chloride hexahydrate, TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine), copper (III) chloride, neocuproine, Folin-Ciocalteu reagent, gallic acid, sodium nitrite, and catechin were acquired from Sigma-Aldrich Co. (St. Louis, MO, USA). All other reagents used in this study were of analytical grade.

Liu F.J., Yang J., Chen X.Y., Yu T., Ni H., Feng L., Li P, & Li H.J. (2022). Chemometrics integrated with in silico pharmacology to reveal antioxidative and anti-inflammatory markers of dandelion for its quality control. Chinese Medicine, 17, 125.

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