Engineered cell sheets were fixed in 10% neutral buffered formalin, embedded in paraffin, and the tissue sections were then stained with hematoxylin and eosin (H&E) or used for immunofluorescent staining using deltaN‐p63 (Biocare Medical, Concord, CA), proliferating cell nuclear antigen (PCNA; DAKO, Carpinteria, CA), Ki67 (ImmunoTech, Commerce, CA), CK3 (ImmuQuest, North Yorkshire, England), CK4, CK13 (Santa Cruz Biotechnology Inc., Santa Cruz, CA), E‐Cadherin, Beta‐Catenin (BD Biosciences, San Jose, CA), and ZO‐1 (ThermoFisher Scientific, Carlsbad, CA). Alexa Fluor® 488 donkey anti‐mouse conjugated second antibodies or Alexa Fluor® 488 donkey anti‐rabbit conjugated second antibodies (Invitrogen, Eugene, OR) were used. Propidium iodine (Invitrogen, Eugene, OR) was used to stain nuclear DNA. A Nikon 400 fluorescent microscope was used to analyze the slides (Nikon Inc., Melville, NY).
Alexa fluor 488 donkey anti rabbit conjugated second antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor® 488 donkey anti‐rabbit conjugated second antibodies are fluorescently labeled secondary antibodies used for protein detection and quantification in various immunoassays. They bind to primary rabbit antibodies and emit green fluorescence upon excitation, allowing visualization and localization of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodine, by Thermo Fisher Scientific (2 mentions)
Deltanp63, by Biocare Medical (1 mentions)
400 fluorescent microscope, by Nikon (1 mentions)
E cadherin, by BD (1 mentions)
β catenin, by BD (1 mentions)
Col 2, by Abcam (1 mentions)
Bglap, by Thermo Fisher Scientific (1 mentions)
Srebp1, by Thermo Fisher Scientific (1 mentions)
Hla a, by Abcam (1 mentions)
Hla dr, by Abcam (1 mentions)
Pparg, by Cell Signaling Technology (1 mentions)
Cd105, by Thermo Fisher Scientific (1 mentions)
2 protocols using alexa fluor 488 donkey anti rabbit conjugated second antibodies
1
Immunofluorescence Staining of Engineered Cell Sheets
2
Immunofluorescent Characterization of Cell Sheets
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Engineered cell sheets were fixed in 10% neutral buffered formalin and embedded in paraffin. Tissue sections were then stained with hematoxylin & eosin (H&E) or used for immunofluorescent staining with CD19, CD73, Acetyl-CoA1 (NovusBIo, CO, USA), CD29, COLII (Collagen II), HLA-A, HLA-DR (Abcam, MA, USA), CD45R, CD105, BGLAP (Osteocalcin), SREBP1 (ThermoFisher Scientific, CA, USA), PPARg (Cell Signaling, MA, USA), ACAN (Aggrecan) and SPARC (ThermoFisher Scientific). Alexa Fluor R 488 donkey antimouse conjugated second antibodies, Alexa Fluor 488 donkey antirabbit conjugated second antibodies and Alexa Fluor 488 donkey antirat conjugated second antibodies (Invitrogen, OR, USA) were used. Propidium iodine (Invitrogen) was used to stain nuclear DNA. A Nikon 400 fluorescence microscope was used to analyze the slides (Nikon Inc., NY, USA).
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