Zebron zb 5ms column

Styrene measurements were performed by HS-SPME coupled to GC–MS. The chromatographic separation was carried out using an Agilent 7890A gas chromatograph (Agilent, USA) with a Zebron™ ZB-5MS column (30 m, 0.23 mm ID, 0.23 μm film; Phenomenex, USA). Helium gas was used as carrier gas at a 1.2 mL min⁻¹ flow rate. The samples were injected in split mode (100:1) and the injector temperature was held at 240 ºC. The column temperature program started at 40 ºC for 2 min and then ramped up to 240 ºC at 10 ºC per min and held there for 2 min. A mass spectrometer detector was used (model Quattro micro GC; Waters, USA) with an electron impact ionization source of 70 eV. The temperatures of the MS source and MS transfer line were set at 240 ºC.
SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS, 50/30 μm, 23 ga) was used for the HS-SPME procedure. The pre-incubation time was 3 min. The incubation was carried out at 40ºC and 500 rpm for 15 min. The desorption temperature was 240 ºC and the desorption time was 2 min.
Styrene and other biotransformation products present in the samples were unequivocally identified by matching the corresponding mass spectra with a standard NIST17 spectral database.

The GC/MS-QP2010 Plus (Shimadzu®) fitted with a Zebron ZB 5MS column (Phenomenex®; 30 m × 0.25 mm × 0.25 μm) was used. An injection temperature of 230 °C was used, and 1 μL of each sample was injected with split 10 mode. The temperature program was initiated with a hold at 40 °C for 1 min, followed by a ramp of 6 °C/min to 210 °C, another 20 °C/min ramp till 330 °C, and a bake-out at 330 °C for 5 min, with helium as a carrier gas with constant linear velocity. The MS was employed with ion source and interface temperatures of 250 °C. A scan range (m/z) of 40–700 with a 0.2 s event time was used. For data processing, the “GCMS solution” software (Shimadzu®) was utilized.

Analyses were performed with the GC TQ8030 system (Shimadzu, Kyoto, Japan) equipped with electron ionization (EI) ion source and Zebron ZB-5MS column (30 m × 0.25 mm, 0.25 μm; Phenomenex, Torrance, CA, USA) with helium as a carrier gas. Sample injection volume was 1 µL and the injector temperature was set to 250 °C. The temperature gradient program was: 60 °C (1 min), 60 320 °C (8 °C/min), 320 °C (5 min), with a total analysis time of 38.5 min. The mass spectrometer was operated in a scan mode with the m/z range from 50 to 600. The ion source voltage and temperature were set to 70 eV and 200 °C, respectively. At the beginning of the sequence run, the mixture of alkanes (from C10 to C40, even carbon number) was analyzed, as it is required for retention indices calculation, retention time alignment and metabolite identification.