Infrared spectroscopy of GG, P, RES, empty beads, and RES-loaded beads was performed using a Vertex 70 Fourier Transform Infrared (FTIR) spectrometer (Bruker Optics Inc., Billerica, MA, USA) equipped with a Golden Gate single reflection ATR accessory and DLaTGS detector to investigate polymer–drug interactions [32 (link),33 (link)]. Powdered samples were scanned over a wave region of 400–4000 cm−1.
Vertex 70 fourier transform infrared ftir spectrometer
Manufactured by Bruker
Sourced in United States, United Kingdom, Germany
The Vertex 70 Fourier Transform Infrared (FTIR) spectrometer is a laboratory instrument designed for the analysis of molecular structures and compositions. It uses infrared radiation to capture the absorption or transmission characteristics of samples, providing detailed information about their chemical properties. The Vertex 70 is capable of performing a wide range of spectroscopic measurements and is suitable for applications in various scientific and industrial fields.
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Lab products found in correlation
μ quant microplate reader, by Edinburgh Instruments (1 mentions)
Fluorescence lifetime spectrometer 1000, by Edinburgh Instruments (1 mentions)
Zetasizer nano instrument, by Malvern Panalytical (1 mentions)
S 4800 scanning electron microscope, by Hitachi (1 mentions)
Gcms qp2010 ultra, by Shimadzu (1 mentions)
Prisma 3.0 t mr scanner, by Siemens (1 mentions)
Tecnai g2 high resolution tem, by Thermo Fisher Scientific (1 mentions)
Elan 9000 drc icp ms system, by PerkinElmer (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
D8 advance diffractometer, by Bruker (1 mentions)
4 protocols using vertex 70 fourier transform infrared ftir spectrometer
1
Infrared Spectroscopy Analysis of Polymer-Drug Interactions
2
Characterization of Nanodiamond Particles
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A Malvern Zetasizer Nano instrument and the associated Zetasizer software were used to determine the hydrodynamic diameter, polydispersity index and zeta potential of particles in water via dynamic light scattering (DLS)( Malvern, UK). Samples were diluted with water (0.1 mg/mL) and sonicated for 10 min prior to DLS analysis. Images of the particles were acquired with a Hitachi S4800 scanning electron microscope (SEM) at 5 kV. For SEM imaging, the nanodiamond suspension was processed by adding a drop of the nanosuspension onto an SEM (Hitachi Europe Ltd, Berkshire, UK) stub and allowing the solvent (water) to evaporate. Solid samples were directly attached to the stub using conductive double-sided carbon tape. Infrared spectroscopic data were obtained using a Vertex 70 Fourier Transform Infrared (FTIR) Spectrometer (Bruker, Brighton, UK). Optical properties were investigated using a μ-Quant Microplate Reader and a Fluorescence Lifetime Spectrometer (FLS) 1000 (Edinburgh Instruments). To obtain fluorescence spectra, samples were prepared at a concentration of 0.1 mg/ml and analysed using a front-face sample holder, 590 nm emission filter and excitation/emission bandwidths of 0.8–3 nm. Excitation wavelengths of 500 or 560 nm were used and emission was measured in the range of 550–800 nm, with a 1 nm step and 0.2 s dwell time.
3
Metabolic Syndrome and Kidney Stones
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This case-control study (duration: 1.5 years) was carried out on a group of 71 men (average age 53.1±14.1) with kidney stone disease; enrolled individuals were hospitalized at Vilnius University Hospital and gave their written consent to participate in the study (case group). The control group had no history of kidney stone disease, and was matched with cases for age and sex (n=100). All patients were thoroughly examined to diagnose MS according to clinical and laboratory criteria [18 (link)]. The kidney stones of patients were removed and the chemical composition of the stones was examined using infrared spectroscopy.
The study protocol was approved by the Vilnius Regional Bioethics Committee (Approval No. 158200-5-053-056LP1).
The chemical composition of the stones was examined by a BRUKER VERTEX 70 Fourier transform infrared (FTIR) spectrometer by using a KBr tablet [19 ].
Methyl esters of adipose tissue FA were prepared using the Folch method [20 (link),21 ] and were identified by gas chromatography–mass spectrometry (GCMS-QP2010 Ultra, Shimadzu) (Table 1 ). Individuals were divided into groups according to diagnosis of (MS) and type of kidney stone (Figure 1 ). The composition of adipose tissue FA was compared within different groups of patients with different types of kidney stones and between the patients and control individuals.
The study protocol was approved by the Vilnius Regional Bioethics Committee (Approval No. 158200-5-053-056LP1).
The chemical composition of the stones was examined by a BRUKER VERTEX 70 Fourier transform infrared (FTIR) spectrometer by using a KBr tablet [19 ].
Methyl esters of adipose tissue FA were prepared using the Folch method [20 (link),21 ] and were identified by gas chromatography–mass spectrometry (GCMS-QP2010 Ultra, Shimadzu) (
4
Comprehensive Characterization of Nanomaterials
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TEM micrographs were recorded by TECNAI G2 high-resolution TEM (FEI Co., USA). Dynamic light scattering and zeta potential of the as-prepared samples were carried out on a Zetasizer Nano ZS (Malvern Instruments Ltd, UK). The analysis of elements was conducted with an ELAN 9000/DRC ICP-MS system (PerkinElmer, USA). The relaxation times of the samples were carried out on a Siemens Prisma 3.0 T MR scanner (Erlangen, Germany). Energy-dispersive X-ray spectra (EDS) were inspected on an energy dispersive spectroscopy (FEI Co., USA). X-ray diffraction analysis were carried out on a D8 ADVANCE diffractometer (Bruker Co., Germany) using Cu Kα (0.15406 nm) radiation. The infrared spectra were conducted with a Vertex 70 Fourier transform infrared (FTIR) spectrometer (Bruker, Germany). XPS measurements were conducted with a VG ESCALAB MKII X-ray photoelectron spectrometer (VG Scientific Ltd, UK) spectroscopy (XPS). Siemens Prisma 3.0 T MR scanner (Erlangen, Germany) was employed to acquire T1-weighted MR images.
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