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Tempplate rt select optical film

Manufactured by USA Scientific
Sourced in United States

TempPlate RT Select Optical Film is a laboratory product designed for use in real-time PCR (RT-PCR) applications. It provides an optical barrier to enhance signal detection and improve overall performance in RT-PCR experiments.

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2 protocols using tempplate rt select optical film

1

Amyloid Aggregation Kinetics Assay

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150 μL of freshly thawed apoLECT2 (same concentration and buffer conditions as in the microfluidic experiments) was added to wells of fully blackened, polystyrene, round bottom 96-well plates (Corning CoStar). Six 1-mm diameter Zirconia/silica beads (BioSpec) and 15 μM ThT were added to each well and the plates were sealed (TempPlate RT Select Optical Film; USA Scientific) to prevent evaporation. Plates were shaken at 650 RPM and 34 °C using a Mixer HC (USA Scientific). At the indicated times, ThT fluorescence was recorded using a SpectraMax i3x plate reader (Molecular Devices) with excitation at 410 nm and emission at 490 nm.
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2

Thioflavin T Assay for Amyloid Aggregation

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150 μl of freshly thawed apoLECT2 (same concentration and buffer conditions as in the microfluidic experiments) was added to wells of fully blackened, polystyrene, round bottom 96-well plates (Corning CoStar). Six 1-mm diameter Zirconia/silica beads (BioSpec) and 15 μM ThT were added to each well and the plates were sealed (TempPlate RT Select Optical Film; USA Scientific) to prevent evaporation. Experiments with glycosaminoglycans contained 5 μg/ml heparin or 5 μg/ml HS. Plates were shaken at 650 RPM and 35 °C using a Mixer HC (USA Scientific). At the indicated times, ThT fluorescence was recorded using a SpectraMax i3x plate reader (Molecular Devices) with excitation at 410 nm and emission at 490 nm.
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