150 μL of freshly thawed apoLECT2 (same concentration and buffer conditions as in the microfluidic experiments) was added to wells of fully blackened, polystyrene, round bottom 96-well plates (Corning CoStar). Six 1-mm diameter Zirconia/silica beads (BioSpec) and 15 μM ThT were added to each well and the plates were sealed (TempPlate RT Select Optical Film; USA Scientific) to prevent evaporation. Plates were shaken at 650 RPM and 34 °C using a Mixer HC (USA Scientific). At the indicated times, ThT fluorescence was recorded using a SpectraMax i3x plate reader (Molecular Devices) with excitation at 410 nm and emission at 490 nm.
Tempplate rt select optical film
Manufactured by USA Scientific
Sourced in United States
TempPlate RT Select Optical Film is a laboratory product designed for use in real-time PCR (RT-PCR) applications. It provides an optical barrier to enhance signal detection and improve overall performance in RT-PCR experiments.
Automatically generated - may contain errors
2 protocols using tempplate rt select optical film
1
Amyloid Aggregation Kinetics Assay
2
Thioflavin T Assay for Amyloid Aggregation
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150 μl of freshly thawed apoLECT2 (same concentration and buffer conditions as in the microfluidic experiments) was added to wells of fully blackened, polystyrene, round bottom 96-well plates (Corning CoStar). Six 1-mm diameter Zirconia/silica beads (BioSpec) and 15 μM ThT were added to each well and the plates were sealed (TempPlate RT Select Optical Film; USA Scientific) to prevent evaporation. Experiments with glycosaminoglycans contained 5 μg/ml heparin or 5 μg/ml HS. Plates were shaken at 650 RPM and 35 °C using a Mixer HC (USA Scientific). At the indicated times, ThT fluorescence was recorded using a SpectraMax i3x plate reader (Molecular Devices) with excitation at 410 nm and emission at 490 nm.
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