The largest database of trusted experimental protocols

Anti bcl11b

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-BCL11B is a primary antibody used in laboratory research applications. It specifically binds to the BCL11B protein, which plays a role in cellular processes. The antibody can be used to detect and analyze the expression of BCL11B in various biological samples.

Automatically generated - may contain errors

2 protocols using anti bcl11b

1

Western Blot Analysis of BCL11B, Tax, and HBZ

Check if the same lab product or an alternative is used in the 5 most similar protocols
The western blot analyses were carried out as described previously.24 (link) The primary antibodies used at the indicated dilutions were anti-BCL11B (1/1000),16 (link) anti-BCL11B (1/1000) (Cell Signaling Technology, Beverly, MA, USA), anti-Tax (1/2000) (Taxy7),25 (link) anti-Tax2,26 (link) anti-α-tubulin (DM1A) (1/1000) (Calbiochem, San Diego, CA, USA) and α-HBZ (1/1000).9 (link) Horseradish peroxidase (HRP)-conjugated rat anti-mouse IgG kappa light chain (BD Biosciences, San Jose, CA, USA) and HRP-conjugated goat anti-rabbit IgG (sc-2054; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies (1/10 000). Immunoreactive bands were visualized with the Enhanced Chemiluminescence Detection System (Amersham Pharmacia Biotech, Orsay, France).
+ Open protocol
+ Expand
2

Western Blot Analysis of HTLV-1 Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The western blot analyses were carried out as described previously.(24 (link)) The primary antibodies used at the indicated dilutions were anti-BCL11B (1/1000),(16 (link)) anti-BCL11B (1/1000) (Cell Signaling Technology, Beverly, MA, USA), anti-Tax (1/2000) (Taxy7),(25 (link)) anti-Tax2,(26 (link)) anti α-tubulin (DM1A) (1/1000) (Calbiochem, San Diego, CA, USA) and α-HBZ (1/1000).(9 (link)) Horseradish peroxidase (HRP)-conjugated rat anti-mouse IgG kappa light chain (BD Biosciences, San Jose, CA, USA) and HRP-conjugated goat anti-rabbit IgG (sc-2054; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies (1/10 000). Immunoreactive bands were visualized with the Enhanced Chemiluminescence Detection System (Amersham Pharmacia Biotech, Orsay, France).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!