Anti phospho akt ser473

Collagen (type I) and thrombin were purchased from Sigma (St. Louis, MO, USA). The anti-phospho-c-Jun N-terminal kinase (JNK) (Thr¹⁸³/Tyr¹⁸⁵), anti-JNK, anti-phospho-p38 mitogen-activated protein kinase (MAPK), anti-p38 MAPK and anti-phospho-Syk (Tyr^525/526) monoclonal antibodies (mAbs) were purchased from Cell Signaling (Beverly, MA, USA). The anti-phospho-Akt (Ser⁴⁷³) and anti-Akt mAbs were purchased from Biovision (Mountain View, CA, USA). Anti-phospho-Fyn (Y⁵³⁰) mAb, anti-phospho-Lyn (Y⁵⁰⁷) mAb was obtained from Abcam (Cambridge, UK). The horseradish peroxidase (HRP) conjugated donkey anti-rabbit immunoglobulin G (IgG), the Hybond-P polyvinylidene difluoride (PVDF) membrane, the sheep anti-mouse IgG and the enhanced chemiluminescence western blotting detection reagent was purchased from Amersham (Buckinghamshire, UK). The TQ1, TQ-2 and TQ-3 were dissolved in DMSO and stored at 4 °C.

Collagen (type I), luciferin-luciferase, arachidonic acid (AA), U46619, PDBu, heparin, prostaglandin E1 (PGE1), 2,3,5-triphenyl tetrazolium chloride (TTC), and thrombin were purchased from Sigma (St. Louis, MO, USA). Fura 2-AM, an intracellular calcium indicator, was purchased from Molecular Probes (Eugene, OR, USA). The anti-phospho-p38 MAPK Ser182 monoclonal antibody (mAb) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-p38 MAPK, anti-phospho-JNK (Thr183/Tyr185), and anti-p44/42 ERK (1/2) mAbs and anti-PLCγ2, anti-phospho (Tyr759) PLCγ2, the anti-phospho-(Ser) PKC substrate, anti-JNK and anti-phospho-p44/p42 ERK (Thr202/Tyr204) polyclonal antibodies (pAbs) were purchased from Cell Signaling (Beverly, MA, USA). The anti-phospho-Akt (Ser473) and anti-Akt mAbs were purchased from Biovision (Mountain View, CA, USA). Anti-pleckstrin (p47) pAb was purchased from GeneTex (Irvine, CA, USA). The anti-α-tubulin mAb was purchased from NeoMarkers (Fremont, CA, USA). A hybond-P PVDF membrane, an enhanced chemiluminescence Western blotting detection reagent, the horseradish peroxidase (HRP)-conjugated donkey anti-rabbit immunoglobulin G (IgG), and the sheep anti-mouse IgG were purchased from Amersham (Buckinghamshire, UK). The Dade Behring PFA-100 CEPI and CADP test cartridges were obtained from Siemens Healthcare (Erlangen, Germany).

Wortmannin, dynasore, SU6655, NH₄Cl, SP600125, IPA-3, MβCD and chlorpromazine were obtained from Sigma (Sigma, MO, United States), Akti-1/2 and bafilomycin A1 were obtained from Abcam (Abcam, Cambridge, United Kingdom), nystatin and EIPA was obtained from Solarbio (Solarbio, Beijing, China).
The rabbit anti-dynamin-2, anti-clathrin heavy chain, anti-Rab5, anti-Rab7, anti-PI3K p85 (phos pho Y458) + PI3 Kinase p55 (phospho Y199), anti-Akt, anti-Src, anti-Src (phospho Y416), anti-phospho-Akt (Ser473) and anti-GAPDH monoclonal antibodies were obtained from Abcam (Abcam, Cambridge, United Kingdom). The rabbit anti-JNK and anti-JNK (Thr183/Tyr185) monoclonal antibodies were obtained from Cell Signaling Technology (Cell Signaling Technology, Danvers, United States). The rabbit anti-PI3 Kinase p85 alpha polyclonal antibody, the mouse anti-Flag monoclonal antibody, goat anti-rabbit and goat anti-mouse HRP-labeled secondary antibody, Alexa fuor-488-conjugated anti-mouse, Cy™3-conjugated anti-rabbit IgG (H + L) and mounting medium with DAPI was purchased from Abcam (Abcam, Cambridge, United Kingdom). The rabbit anti-caveolin-1 monoclonal antibody was obtained from Beyotime (Beyotime Biotechnology, Shanghai, China). The mouse anti-BRSV G protein monoclonal antibody was provided by China Animal Health and Epidemiology Center.