Mouse hearts were isolated, and 4%-PFA fixed-left ventricles were sliced at 7 μm and stained using a Hematoxylin-Eosin Stain Kit and Picro-Sirius Red Stain Kit (COSMO BIO, Japan). Fibrosis was imaged using a BZ-X-710 microscope (KEYENCE, Japan), and fibrotic areas in each cross-section of the left ventricle at the papillary muscle level were quantified using the automated calculation software of the BZ-X-Analyzer software (KEYENCE, Japan). Autophagosome was stained with LC3 (Santa Cruz, sc-28266) and automatically counted the number of LC3-positive dots by using a BZ-X-Analyzer software and Photoshop software (Adobe).
Hematoxylin eosin stain kit
Manufactured by Cosmo Bio
Sourced in Japan
The Hematoxylin-Eosin Stain Kit is a laboratory reagent used for staining and visualizing cell and tissue structures in microscopy. The kit contains hematoxylin and eosin dyes, which stain nucleic acids and proteins respectively, allowing for the differentiation of cellular components.
Automatically generated - may contain errors
2 protocols using hematoxylin eosin stain kit
1
Cardiac Fibrosis and Autophagy Analysis
2
Cardiac Fibrosis and Autophagy Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse hearts were isolated, and 4%-PFA xed left ventricles were sliced at 7 µm and stained using a Hematoxylin-Eosin Stain Kit and Picro-Sirius Red Stain Kit (COSMO BIO, Japan). Fibrosis was imaged using a BZ-9000 microscope, and brotic areas were quanti ed using the automated calculation software of the BZ-9000 (KEYENCE, Japan). Transduction of green/red uorescent protein-LC3 (GFP-mRFP-LC3) (COSMO BIO) was performed by lipofection into left ventricular sections. The number of green uorescent dots, representing autophagosomes, were counted with the BZ-9000 microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!