Fifty μg quantity of protein was separated on SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). Membranes were then blocked with 5% evaporated skimmed milk (Bio-rad, USA) in Tris-buffered saline (50 mM Tris-HCl, pH 7.5, 150 mM NaCl) containing 0.1% Tween-20 for 1 h, and probed overnight at 4 °C with the following primary antibodies: antibodies against human LC3B, SQSTM1/P62, HSP60 (1:1000; Cell Signaling Technology, CST), antibody against RAB7A, RILP, EEA1, LAMP2, ACTB (1:1000, Proteintech, China), antibody against LAMP1 (1:500; Santa Cruz, USA), followed by incubation with horseradish peroxidase coupled secondary anti-mouse or anti-rabbit antibodies (Proteintech) for 1 h at room temperature. The protein bands were visualized using ECL blotting detection reagents (Bio-rad, USA), and developed and fixed onto x-ray films. ACTB was served as a loading control.
Antibody against lamp1
Manufactured by Santa Cruz Biotechnology
Sourced in China
The Antibody against LAMP1 is a laboratory tool used to detect the presence and localization of the LAMP1 (Lysosome-Associated Membrane Protein 1) protein in cells. LAMP1 is a commonly used marker for lysosomes, which are organelles involved in the degradation and recycling of cellular components. This antibody can be utilized in various research applications, such as immunohistochemistry, immunocytochemistry, and Western blotting, to study the expression and distribution of LAMP1 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Evaporated skimmed milk, by Bio-Rad (1 mentions)
Ecl blotting detection reagents, by Bio-Rad (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Dimethyl sulphoxide, by Merck Group (1 mentions)
Lyso tracker red, by Beyotime (1 mentions)
Arf 6, by Cell Signaling Technology (1 mentions)
Clathrin, by Cell Signaling Technology (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Cdc42, by Cell Signaling Technology (1 mentions)
Antibody against β actin, by Abmart (1 mentions)
2 protocols using antibody against lamp1
1
Western Blot Analysis of Autophagy Markers
2
Cellular Endocytosis Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bovine serum albumin, dimethyl sulphoxide (DMSO), Chloroquine (CQ), and 3-methyladenine (3-MA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-acryloxysuccinimide (NAS), acrylamide (AAm), N, N-methylene bisacrylamide (BIS), ammonium persulphate (APS), N, N, N', N'-tetramethylethylenediamine (TEMED) and Fluorescein isothiocyanate (FITC) were purchased from Aladdin Industrial Co. LTD. (Shanghai, China). N-(3-Aminopropyl) methacrylamide hydrochloride was purchased from Polymer Science, Inc. All other chemicals of the highest quality were commercially available and used as received. Lyso-Tracker Red was purchased from Beyotime Biotechnology (Shanghai, China). Antibodies against LC3, Arf-6, Flotillin, Cdc42, RhoA, P62, EEA1, Clathrin, Caveolin were from Cell Signaling Technology. The antibody against LAMP1 was from Santa Cruz Biotechnologies, and the antibody against β-actin was obtained from Abmart.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!