alpha-PBP and alpha-PEP were provided by the Bavarian State Criminal Police Office (Munich, Germany). NADP-Na2, acetonitrile (LC-MS grade), and methanol (LC-MS grade) were obtained from VWR (Darmstadt, Germany), MgCl2, K2HPO4, K3PO4, superoxide dismutase, isocitrate dehydrogenase, isocitrate, ammonium formate, ammonium acetate, and formic acid from Sigma (Taufkirchen, Germany). Water was purified with a Millipore filtration unit (18.2 Ω × cm water resistance). pHLM (pool of 25 donors, 20 mg microsomal protein/mL) were obtained from Corning (Amsterdam, The Netherlands). After delivery, the pHLM were thawed at 37 °C, aliquoted, snap-frozen in liquid nitrogen, and stored at −80 °C until use.
Nadp na2
Manufactured by Avantor
Sourced in Germany
NADP-Na2 is a chemical compound that serves as a cofactor in various enzymatic reactions. It is the disodium salt of nicotinamide adenine dinucleotide phosphate (NADP). NADP-Na2 is commonly used in biochemical and enzymatic assays.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium acetate, by Merck Group (4 mentions)
Ammonium formate, by Merck Group (4 mentions)
Superoxide dismutase, by Merck Group (4 mentions)
Isocitrate dehydrogenase, by Merck Group (4 mentions)
Formic acid, by Merck Group (4 mentions)
Isocitrate, by Merck Group (3 mentions)
Lc ms grade acetonitrile, by Avantor (2 mentions)
Lc ms grade methanol, by Avantor (2 mentions)
K2hpo4, by Merck Group (2 mentions)
Mgcl2, by Merck Group (2 mentions)
Dipotassium phosphate, by Merck Group (2 mentions)
Magnesium chloride, by Merck Group (2 mentions)
K3po4, by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
Filtration unit, by Merck Group (1 mentions)
Creatinine d3, by Merck Group (1 mentions)
Ethanol, by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Phlms, by Corning (1 mentions)
4 protocols using nadp na2
1
Quantitative Analysis of Novel Psychoactive Substances
2
Analytical Verification of A-CHMINACA
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A-CHMINACA was provided by the EU project ADEBAR/State Bureau of Criminal Investigation Schleswig-Holstein (Kiel, Germany) for research purpose. The chemical purity and identity of the compound were verified by MS and nuclear magnetic resonance analysis. Ammonium formate, ammonium acetate, formic acid, isocitrate dehydrogenase, isocitrate, dipotassium phosphate, tripotassium phosphate, magnesium chloride, and superoxide dismutase were obtained from Sigma (Taufkirchen, Germany). Acetonitrile (LC-MS grade), methanol (LC-MS grade), and NADP-Na2 were from VWR (Darmstadt, Germany). pHLM (20 mg microsomal protein mL−1) was obtained from Corning (Amsterdam, The Netherlands). After delivery, pHLM were thawed at 37 °C, aliquoted, snap-frozen in liquid nitrogen, and stored at −80 °C until use.
3
Preparation and Characterization of Hydrochloric Acid Gas in 2-Propanol
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All chemicals used for syntheses were obtained from Sigma Aldrich (Taufkirchen, Germany). Hydrochloric acid gas in 2-propanol was prepared by gassing isopropanol with hydrogen chloride (generated from the reaction between conc. H2SO4 and sodium chloride) in the laboratory. NADP-Na2, acetonitrile (LC-MS grade), and methanol (LC-MS grade) were obtained from VWR (Darmstadt, Germany), MgCl2, K2HPO4, KH2PO4, superoxide dismutase, isocitrate dehydrogenase, isocitrate, ammonium formate, ammonium acetate, and formic acid from Sigma (Taufkirchen, Germany). Water was purified with a Millipore filtration unit (18.2 Ω × cm water resistance). pHLM (pool of 25 donors, 20 mg microsomal protein/mL) were obtained from Corning (Amsterdam, The Netherlands). After delivery, the pHLM were aliquoted, snap-frozen in liquid nitrogen, and stored at −80 °C until use.
4
Quantitative PCYP Metabolic Analysis
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PCYP hydrochloride was provided by the State Bureau of Criminal Investigation Schleswig-Holstein (E.U. project ADEBAR plus, Kiel, Germany) for research purposes. The chemical purity of >93% and the identity of the compound was verified by MS and nuclear magnetic resonance analysis. Ammonium formate, ammonium acetate, creatinine-d3, dipotassium phosphate, formic acid, D-glucose-1,2,3,4,5,6,6-d7, isocitrate dehydrogenase, isocitrate, magnesium chloride, palmitic acid-d31, superoxide dismutase, and tripotassium phosphate were obtained from Merck (Darmstadt, Germany). Acetonitrile, ethanol, methanol (all LC-MS grade), and NADP-Na2 were from VWR (Darmstadt, Germany). L-Tryptophan-d5 was obtained from Alsachim (Illkirch-Graffenstaden, France). 1-Palmitoyl-d9-2-palmitoyl-sn-glycero-3-PC and prostaglandin-E3-d9 were from Cayman Chemical (Ann Arbor, MI, USA). Water was purified with a millipore filtration unit (18.2 W × cm water resistance). pHLMs (20 mg microsomal protein × mL−1, 360 pmol total CYP/mg, 26 donors) were obtained from Corning (Amsterdam, The Netherlands). After delivery, pHLMs were thawed at 37 °C, aliquoted, snap-frozen in liquid nitrogen, and stored at −80 °C until use.
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