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Angiotensin 2 eia kit

Manufactured by Cayman Chemical
Sourced in France

The Angiotensin II EIA kit is a laboratory tool used to quantify the levels of angiotensin II, a peptide hormone that plays a role in regulating blood pressure and fluid balance within the body. The kit utilizes an enzyme-linked immunosorbent assay (EIA) technique to detect and measure the concentration of angiotensin II in a given sample.

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3 protocols using angiotensin 2 eia kit

1

Quantification of Angiotensin II and C-peptide

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The determination of plasma levels of angiotensin II and peptide C, as a measure of insulin concentration, was conducted by ELISA kit for angiotensin II (Angiotensin II EIA kit, Cayman) or ELISA kit for peptide C (Human C-peptide ELISA, Millipore) following the manufacturer's recommendations.
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2

Angiotensin II Quantification in Kidney

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At the end of the study, urine samples were collected into tubes containing an inhibitor cocktail (5 mmol/L of EDTA, 20 μmol/L of pepstatin A, 10 μmol/L of PMSF, 20 μmol/L of enalaprilat, and 1.25 mmol/L of 1,10-phenanthroline). Left kidneys were decapsulated and immersed in cold 100% methanol. After homogenization, centrifugation and evaporation the samples were extracted and evaporated as described previously20 . Angiotensin II concentrations were measured by EIA (Angiotensin II EIA Kit, Cayman, catalog #A05880, Montigny-le-Bretonneux, France) and expressed per gram of wet weight. Urinary excretion rates were calculated from the 12 h volumes collected.
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3

Measuring Plasma Angiotensin II and C-Peptide

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The determination of plasma levels of angiotensin II and peptide C -as a measure of insulin concentration -was conducted by ELISA kit for Ang II (Angiotensin II EIA kit, Cayman®) or ELISA kit for peptide C (Human C-peptide ELISA, Millipore®) following the manufacturer's recommendations.
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